New Insights Into Functions and Possible Applications of Clostridium difficile CRISPR-Cas System
Over the last decades the enteric bacterium Clostridium difficile (novel name Clostridioides difficile) – has emerged as an important human nosocomial pathogen. It is a leading cause of hospital-acquired diarrhea and represents a major challenge for healthcare providers. Many aspects of C. difficile...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-07-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2018.01740/full |
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| author | Anna Maikova Anna Maikova Anna Maikova Anna Maikova Konstantin Severinov Konstantin Severinov Konstantin Severinov Olga Soutourina Olga Soutourina |
| author_facet | Anna Maikova Anna Maikova Anna Maikova Anna Maikova Konstantin Severinov Konstantin Severinov Konstantin Severinov Olga Soutourina Olga Soutourina |
| author_sort | Anna Maikova |
| collection | DOAJ |
| description | Over the last decades the enteric bacterium Clostridium difficile (novel name Clostridioides difficile) – has emerged as an important human nosocomial pathogen. It is a leading cause of hospital-acquired diarrhea and represents a major challenge for healthcare providers. Many aspects of C. difficile pathogenesis and its evolution remain poorly understood. Efficient defense systems against phages and other genetic elements could have contributed to the success of this enteropathogen in the phage-rich gut communities. Recent studies demonstrated the presence of an active CRISPR (clustered regularly interspaced short palindromic repeats)-Cas (CRISPR-associated) subtype I-B system in C. difficile. In this mini-review, we will discuss the recent advances in characterization of original features of the C. difficile CRISPR-Cas system in laboratory and clinical strains, as well as interesting perspectives for our understanding of this defense system function and regulation in this important enteropathogen. This knowledge will pave the way for the development of promising biotechnological and therapeutic tools in the future. Possible applications for the C. difficile strain monitoring and genotyping, as well as for CRISPR-based genome editing and antimicrobials are also discussed. |
| first_indexed | 2024-12-23T21:06:53Z |
| format | Article |
| id | doaj.art-6cd406f408a048c188f90d160bfdeaa6 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-23T21:06:53Z |
| publishDate | 2018-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-6cd406f408a048c188f90d160bfdeaa62022-12-21T17:31:12ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2018-07-01910.3389/fmicb.2018.01740402201New Insights Into Functions and Possible Applications of Clostridium difficile CRISPR-Cas SystemAnna Maikova0Anna Maikova1Anna Maikova2Anna Maikova3Konstantin Severinov4Konstantin Severinov5Konstantin Severinov6Olga Soutourina7Olga Soutourina8Center for Life Sciences, Skolkovo Institute of Science and Technology, Moscow, RussiaUniversité Paris Diderot, Sorbonne Paris Cité, Paris, FranceMicrobiology, Institute for Integrative Biology of the Cell, Commissariat à l’Energie Atomique et aux Energies Alternatives, Centre National de la Recherche Scientifique, Université Paris-Sud, Université Paris-Saclay, Gif-sur-Yvette, FrancePeter the Great St. Petersburg Polytechnic University, Saint Petersburg, RussiaCenter for Life Sciences, Skolkovo Institute of Science and Technology, Moscow, RussiaPeter the Great St. Petersburg Polytechnic University, Saint Petersburg, RussiaWaksman Institute for Microbiology, Rutgers, The State University of New Jersey, Piscataway, NJ, United StatesMicrobiology, Institute for Integrative Biology of the Cell, Commissariat à l’Energie Atomique et aux Energies Alternatives, Centre National de la Recherche Scientifique, Université Paris-Sud, Université Paris-Saclay, Gif-sur-Yvette, FranceInstitut Pasteur, Paris, FranceOver the last decades the enteric bacterium Clostridium difficile (novel name Clostridioides difficile) – has emerged as an important human nosocomial pathogen. It is a leading cause of hospital-acquired diarrhea and represents a major challenge for healthcare providers. Many aspects of C. difficile pathogenesis and its evolution remain poorly understood. Efficient defense systems against phages and other genetic elements could have contributed to the success of this enteropathogen in the phage-rich gut communities. Recent studies demonstrated the presence of an active CRISPR (clustered regularly interspaced short palindromic repeats)-Cas (CRISPR-associated) subtype I-B system in C. difficile. In this mini-review, we will discuss the recent advances in characterization of original features of the C. difficile CRISPR-Cas system in laboratory and clinical strains, as well as interesting perspectives for our understanding of this defense system function and regulation in this important enteropathogen. This knowledge will pave the way for the development of promising biotechnological and therapeutic tools in the future. Possible applications for the C. difficile strain monitoring and genotyping, as well as for CRISPR-based genome editing and antimicrobials are also discussed.https://www.frontiersin.org/article/10.3389/fmicb.2018.01740/fullCRISPRC. difficileI-B subtype CRISPR-Cas systemprophageCRISPR regulationstress |
| spellingShingle | Anna Maikova Anna Maikova Anna Maikova Anna Maikova Konstantin Severinov Konstantin Severinov Konstantin Severinov Olga Soutourina Olga Soutourina New Insights Into Functions and Possible Applications of Clostridium difficile CRISPR-Cas System Frontiers in Microbiology CRISPR C. difficile I-B subtype CRISPR-Cas system prophage CRISPR regulation stress |
| title | New Insights Into Functions and Possible Applications of Clostridium difficile CRISPR-Cas System |
| title_full | New Insights Into Functions and Possible Applications of Clostridium difficile CRISPR-Cas System |
| title_fullStr | New Insights Into Functions and Possible Applications of Clostridium difficile CRISPR-Cas System |
| title_full_unstemmed | New Insights Into Functions and Possible Applications of Clostridium difficile CRISPR-Cas System |
| title_short | New Insights Into Functions and Possible Applications of Clostridium difficile CRISPR-Cas System |
| title_sort | new insights into functions and possible applications of clostridium difficile crispr cas system |
| topic | CRISPR C. difficile I-B subtype CRISPR-Cas system prophage CRISPR regulation stress |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2018.01740/full |
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