A Modified Method for Measuring Nτ-Methylhistidine in Chiken Feed and Excreta
Analysis of Nτ-methylhistidine is intricate and time consuming. In the present study, a modified technique using high-performance liquid chromatography, which utilizes a reversed-phase separation with ion pairing and post column fluorescence derivatization for the analysis of Nτ-meth...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Japan Poultry Science Association
2001-10-01
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| Series: | The Journal of Poultry Science |
| Subjects: | |
| Online Access: | https://www.jstage.jst.go.jp/article/jpsa/38/4/38_4_354/_pdf/-char/en |
| _version_ | 1797817064975499264 |
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| author | Yahya Z. Eid Akira Ohtsuka Kunioki Hayashi |
| author_facet | Yahya Z. Eid Akira Ohtsuka Kunioki Hayashi |
| author_sort | Yahya Z. Eid |
| collection | DOAJ |
| description | Analysis of Nτ-methylhistidine is intricate and time consuming. In the present study, a modified technique using high-performance liquid chromatography, which utilizes a reversed-phase separation with ion pairing and post column fluorescence derivatization for the analysis of Nτ-methylhistidine in chicken feed and excreta is described. Two ml of the hydrolyzed sample was applied directly into the ion-exchange resin column without evaporation of HCl to roughly separate Nτ-methylhistidine from acidic and neutral amino acids. The Nτ-methylhistidine fraction was eluted with 1M pyridine and then evaporated, and the residue was dissolved in the mobile phase and subjected to high-performance liquid chromatography. The present method shortened the time needed to complete the assay by about 30min per sample and improved the separation of Nτ-methylhistidine. |
| first_indexed | 2024-03-13T08:47:03Z |
| format | Article |
| id | doaj.art-6cda17bc6b454c2a9bd8a41ad6898862 |
| institution | Directory Open Access Journal |
| issn | 1346-7395 1349-0486 |
| language | English |
| last_indexed | 2024-03-13T08:47:03Z |
| publishDate | 2001-10-01 |
| publisher | Japan Poultry Science Association |
| record_format | Article |
| series | The Journal of Poultry Science |
| spelling | doaj.art-6cda17bc6b454c2a9bd8a41ad68988622023-05-30T00:25:17ZengJapan Poultry Science AssociationThe Journal of Poultry Science1346-73951349-04862001-10-0138435435710.2141/jpsa.38.354jpsaA Modified Method for Measuring Nτ-Methylhistidine in Chiken Feed and ExcretaYahya Z. Eid0Akira Ohtsuka1Kunioki Hayashi2Department of Biochemical Science and Technology, Faculty of Agriculture, Kagoshima UniversityDepartment of Biochemical Science and Technology, Faculty of Agriculture, Kagoshima UniversityDepartment of Biochemical Science and Technology, Faculty of Agriculture, Kagoshima UniversityAnalysis of Nτ-methylhistidine is intricate and time consuming. In the present study, a modified technique using high-performance liquid chromatography, which utilizes a reversed-phase separation with ion pairing and post column fluorescence derivatization for the analysis of Nτ-methylhistidine in chicken feed and excreta is described. Two ml of the hydrolyzed sample was applied directly into the ion-exchange resin column without evaporation of HCl to roughly separate Nτ-methylhistidine from acidic and neutral amino acids. The Nτ-methylhistidine fraction was eluted with 1M pyridine and then evaporated, and the residue was dissolved in the mobile phase and subjected to high-performance liquid chromatography. The present method shortened the time needed to complete the assay by about 30min per sample and improved the separation of Nτ-methylhistidine.https://www.jstage.jst.go.jp/article/jpsa/38/4/38_4_354/_pdf/-char/ennτ-methylhistidinehigh-performance liquid chromatography |
| spellingShingle | Yahya Z. Eid Akira Ohtsuka Kunioki Hayashi A Modified Method for Measuring Nτ-Methylhistidine in Chiken Feed and Excreta The Journal of Poultry Science nτ-methylhistidine high-performance liquid chromatography |
| title | A Modified Method for Measuring Nτ-Methylhistidine in Chiken Feed and Excreta |
| title_full | A Modified Method for Measuring Nτ-Methylhistidine in Chiken Feed and Excreta |
| title_fullStr | A Modified Method for Measuring Nτ-Methylhistidine in Chiken Feed and Excreta |
| title_full_unstemmed | A Modified Method for Measuring Nτ-Methylhistidine in Chiken Feed and Excreta |
| title_short | A Modified Method for Measuring Nτ-Methylhistidine in Chiken Feed and Excreta |
| title_sort | modified method for measuring n tau methylhistidine in chiken feed and excreta |
| topic | nτ-methylhistidine high-performance liquid chromatography |
| url | https://www.jstage.jst.go.jp/article/jpsa/38/4/38_4_354/_pdf/-char/en |
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