Preparation of carrierized cellulase Cellulase@MIL-88B(Fe) and its enzymatic properties
In this study, cellulase was modified by immobilization technique using MIL-88B(Fe) as the carrier, and the immobilization temperature, time, cellulase concentration and pH of 30°C, 6 h, 1.2 mg/mL and 5.0, respectively, were investigated as the optimal preparation conditions. Characterization analys...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2022-07-01
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| Series: | Green Chemistry Letters and Reviews |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/17518253.2022.2124890 |
| _version_ | 1798015853536477184 |
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| author | Erhong Zhang Meng Wang Xiaoliang Pan Xinfeng Wang Wenju Zhang |
| author_facet | Erhong Zhang Meng Wang Xiaoliang Pan Xinfeng Wang Wenju Zhang |
| author_sort | Erhong Zhang |
| collection | DOAJ |
| description | In this study, cellulase was modified by immobilization technique using MIL-88B(Fe) as the carrier, and the immobilization temperature, time, cellulase concentration and pH of 30°C, 6 h, 1.2 mg/mL and 5.0, respectively, were investigated as the optimal preparation conditions. Characterization analysis using FT-IR, XRD and TGA revealed that the successful immobilization of cellulase on MIL-88B(Fe) did not disrupt the integrity of the carrier lattice structure. Compared with the free enzyme, Cellulase@MIL-88B(Fe) maintained 78% and 85.21% of its activity after 10 cycles and 49 d, respectively, and its t1/2 and Ed values were higher than those of the free enzyme, and it was able to maintain a higher activity over a wider pH and temperature range, indicating its higher thermal stability and heat-loss activity. In addition, the Km of Cellulase@MIL-88B(Fe) (1.46 mg/mL) was slightly higher than that of free cellulase (1.41 mg/mL), and Cellulase@MIL-88B(Fe) was able to be applied in the actual enzymatic digestion of corn cobs. |
| first_indexed | 2024-04-11T15:41:24Z |
| format | Article |
| id | doaj.art-6cdd74554e2a42f683c9da3820f6d691 |
| institution | Directory Open Access Journal |
| issn | 1751-8253 1751-7192 |
| language | English |
| last_indexed | 2024-04-11T15:41:24Z |
| publishDate | 2022-07-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Green Chemistry Letters and Reviews |
| spelling | doaj.art-6cdd74554e2a42f683c9da3820f6d6912022-12-22T04:15:47ZengTaylor & Francis GroupGreen Chemistry Letters and Reviews1751-82531751-71922022-07-0115362763710.1080/17518253.2022.2124890Preparation of carrierized cellulase Cellulase@MIL-88B(Fe) and its enzymatic propertiesErhong Zhang0Meng Wang1Xiaoliang Pan2Xinfeng Wang3Wenju Zhang4Shihezi University, Xinjiang, People’s Republic of ChinaShihezi University, Xinjiang, People’s Republic of ChinaShihezi University, Xinjiang, People’s Republic of ChinaShihezi University, Xinjiang, People’s Republic of ChinaShihezi University, Xinjiang, People’s Republic of ChinaIn this study, cellulase was modified by immobilization technique using MIL-88B(Fe) as the carrier, and the immobilization temperature, time, cellulase concentration and pH of 30°C, 6 h, 1.2 mg/mL and 5.0, respectively, were investigated as the optimal preparation conditions. Characterization analysis using FT-IR, XRD and TGA revealed that the successful immobilization of cellulase on MIL-88B(Fe) did not disrupt the integrity of the carrier lattice structure. Compared with the free enzyme, Cellulase@MIL-88B(Fe) maintained 78% and 85.21% of its activity after 10 cycles and 49 d, respectively, and its t1/2 and Ed values were higher than those of the free enzyme, and it was able to maintain a higher activity over a wider pH and temperature range, indicating its higher thermal stability and heat-loss activity. In addition, the Km of Cellulase@MIL-88B(Fe) (1.46 mg/mL) was slightly higher than that of free cellulase (1.41 mg/mL), and Cellulase@MIL-88B(Fe) was able to be applied in the actual enzymatic digestion of corn cobs.https://www.tandfonline.com/doi/10.1080/17518253.2022.2124890Cellulaseimmobilizationrigiditystabilityenzymatic hydrolysis |
| spellingShingle | Erhong Zhang Meng Wang Xiaoliang Pan Xinfeng Wang Wenju Zhang Preparation of carrierized cellulase Cellulase@MIL-88B(Fe) and its enzymatic properties Green Chemistry Letters and Reviews Cellulase immobilization rigidity stability enzymatic hydrolysis |
| title | Preparation of carrierized cellulase Cellulase@MIL-88B(Fe) and its enzymatic properties |
| title_full | Preparation of carrierized cellulase Cellulase@MIL-88B(Fe) and its enzymatic properties |
| title_fullStr | Preparation of carrierized cellulase Cellulase@MIL-88B(Fe) and its enzymatic properties |
| title_full_unstemmed | Preparation of carrierized cellulase Cellulase@MIL-88B(Fe) and its enzymatic properties |
| title_short | Preparation of carrierized cellulase Cellulase@MIL-88B(Fe) and its enzymatic properties |
| title_sort | preparation of carrierized cellulase cellulase mil 88b fe and its enzymatic properties |
| topic | Cellulase immobilization rigidity stability enzymatic hydrolysis |
| url | https://www.tandfonline.com/doi/10.1080/17518253.2022.2124890 |
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