SNP in Potentially Defunct Tetrahydrocannabinolic Acid Synthase Is a Marker for Cannabigerolic Acid Dominance in <i>Cannabis sativa</i> L.
The regulation of cannabinoid synthesis in <i>Cannabis sativa</i> is of increasing research interest as restrictions around the globe loosen to allow the plant’s legal cultivation. Of the major cannabinoids, the regulation of cannabigerolic acid (CBGA) production is the least understood....
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-02-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/12/2/228 |
| _version_ | 1797414934833790976 |
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| author | Andrea R. Garfinkel Matthew Otten Seth Crawford |
| author_facet | Andrea R. Garfinkel Matthew Otten Seth Crawford |
| author_sort | Andrea R. Garfinkel |
| collection | DOAJ |
| description | The regulation of cannabinoid synthesis in <i>Cannabis sativa</i> is of increasing research interest as restrictions around the globe loosen to allow the plant’s legal cultivation. Of the major cannabinoids, the regulation of cannabigerolic acid (CBGA) production is the least understood. The purpose of this study was to elucidate the inheritance of CBGA dominance in <i>C. sativa</i> and describe a marker related to this chemotype. We produced two crossing populations, one between a CBGA dominant cultivar and a tetrahydrocannabinolic acid (THCA) dominant cultivar, and one between a CBGA dominant cultivar and a cannabidiolic acid (CBDA) cultivar. Chemical and genotyping analyses confirmed that CBGA dominance is inherited as a single recessive gene, potentially governed by a non-functioning allelic variant of the THCA synthase. The “null” THCAS synthase contains a single nucleotide polymorphism (SNP) that may render the synthase unable to convert CBGA to THCA leading to the accumulation of CBGA. This SNP can be reliably used as a molecular marker for CBGA dominance in the selection and breeding of <i>C. sativa</i>. |
| first_indexed | 2024-03-09T05:42:12Z |
| format | Article |
| id | doaj.art-6cdec2b870b34452b4292d1bbc19e96f |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-09T05:42:12Z |
| publishDate | 2021-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Genes |
| spelling | doaj.art-6cdec2b870b34452b4292d1bbc19e96f2023-12-03T12:24:20ZengMDPI AGGenes2073-44252021-02-0112222810.3390/genes12020228SNP in Potentially Defunct Tetrahydrocannabinolic Acid Synthase Is a Marker for Cannabigerolic Acid Dominance in <i>Cannabis sativa</i> L.Andrea R. Garfinkel0Matthew Otten1Seth Crawford2Oregon CBD, Independence, OR 97351, USAOregon CBD, Independence, OR 97351, USAOregon CBD, Independence, OR 97351, USAThe regulation of cannabinoid synthesis in <i>Cannabis sativa</i> is of increasing research interest as restrictions around the globe loosen to allow the plant’s legal cultivation. Of the major cannabinoids, the regulation of cannabigerolic acid (CBGA) production is the least understood. The purpose of this study was to elucidate the inheritance of CBGA dominance in <i>C. sativa</i> and describe a marker related to this chemotype. We produced two crossing populations, one between a CBGA dominant cultivar and a tetrahydrocannabinolic acid (THCA) dominant cultivar, and one between a CBGA dominant cultivar and a cannabidiolic acid (CBDA) cultivar. Chemical and genotyping analyses confirmed that CBGA dominance is inherited as a single recessive gene, potentially governed by a non-functioning allelic variant of the THCA synthase. The “null” THCAS synthase contains a single nucleotide polymorphism (SNP) that may render the synthase unable to convert CBGA to THCA leading to the accumulation of CBGA. This SNP can be reliably used as a molecular marker for CBGA dominance in the selection and breeding of <i>C. sativa</i>.https://www.mdpi.com/2073-4425/12/2/228cannabinoidscannabinoid synthesisCBGhemp |
| spellingShingle | Andrea R. Garfinkel Matthew Otten Seth Crawford SNP in Potentially Defunct Tetrahydrocannabinolic Acid Synthase Is a Marker for Cannabigerolic Acid Dominance in <i>Cannabis sativa</i> L. Genes cannabinoids cannabinoid synthesis CBG hemp |
| title | SNP in Potentially Defunct Tetrahydrocannabinolic Acid Synthase Is a Marker for Cannabigerolic Acid Dominance in <i>Cannabis sativa</i> L. |
| title_full | SNP in Potentially Defunct Tetrahydrocannabinolic Acid Synthase Is a Marker for Cannabigerolic Acid Dominance in <i>Cannabis sativa</i> L. |
| title_fullStr | SNP in Potentially Defunct Tetrahydrocannabinolic Acid Synthase Is a Marker for Cannabigerolic Acid Dominance in <i>Cannabis sativa</i> L. |
| title_full_unstemmed | SNP in Potentially Defunct Tetrahydrocannabinolic Acid Synthase Is a Marker for Cannabigerolic Acid Dominance in <i>Cannabis sativa</i> L. |
| title_short | SNP in Potentially Defunct Tetrahydrocannabinolic Acid Synthase Is a Marker for Cannabigerolic Acid Dominance in <i>Cannabis sativa</i> L. |
| title_sort | snp in potentially defunct tetrahydrocannabinolic acid synthase is a marker for cannabigerolic acid dominance in i cannabis sativa i l |
| topic | cannabinoids cannabinoid synthesis CBG hemp |
| url | https://www.mdpi.com/2073-4425/12/2/228 |
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