Effects of Dietary Protein Intake on Cutaneous and Systemic Inflammation in Mice with Acute Experimental Psoriasis
Background: Psoriasis is a systemic inflammatory disorder, primarily characterized by skin plaques. It is linked to co-morbidities including cardiovascular disease and metabolic syndrome. Several studies demonstrate that dietary habits can influence psoriasis development and severity. However, the e...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-05-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/13/6/1897 |
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| author | Tanja Knopp Tabea Bieler Rebecca Jung Julia Ringen Michael Molitor Annika Jurda Thomas Münzel Ari Waisman Philip Wenzel Susanne Helena Karbach Johannes Wild |
| author_facet | Tanja Knopp Tabea Bieler Rebecca Jung Julia Ringen Michael Molitor Annika Jurda Thomas Münzel Ari Waisman Philip Wenzel Susanne Helena Karbach Johannes Wild |
| author_sort | Tanja Knopp |
| collection | DOAJ |
| description | Background: Psoriasis is a systemic inflammatory disorder, primarily characterized by skin plaques. It is linked to co-morbidities including cardiovascular disease and metabolic syndrome. Several studies demonstrate that dietary habits can influence psoriasis development and severity. However, the effect of different dietary protein levels on psoriasis development and severity is poorly understood. In this study, we examine the influence of dietary protein on psoriasis-like skin disease in mice. Methods: We fed male C57BL/6J mice with regular, low protein and high protein chow for 4 weeks. Afterwards, we induced psoriasis-like skin disease by topical imiquimod (IMQ)-treatment on ear and back skin. The local cutaneous and systemic inflammatory response was investigated using flow cytometry analysis, histology and quantitative rt-PCR. Results: After 5 days of IMQ-treatment, both diets reduced bodyweight in mice, whereas only the high protein diet slightly aggravated IMQ-induced skin inflammation. IMQ-treatment induced infiltration of myeloid cells, neutrophils, and monocytes/macrophages into skin and spleen independently of diet. After IMQ-treatment, circulating neutrophils and reactive oxygen species were increased in mice on low and high protein diets. Conclusion: Different dietary protein levels had no striking effect on IMQ-induced psoriasis but aggravated the systemic pro-inflammatory phenotype. |
| first_indexed | 2024-03-10T10:49:46Z |
| format | Article |
| id | doaj.art-6ce699d048f94194bfc0c0390dbe78f0 |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-10T10:49:46Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-6ce699d048f94194bfc0c0390dbe78f02023-11-21T22:17:11ZengMDPI AGNutrients2072-66432021-05-01136189710.3390/nu13061897Effects of Dietary Protein Intake on Cutaneous and Systemic Inflammation in Mice with Acute Experimental PsoriasisTanja Knopp0Tabea Bieler1Rebecca Jung2Julia Ringen3Michael Molitor4Annika Jurda5Thomas Münzel6Ari Waisman7Philip Wenzel8Susanne Helena Karbach9Johannes Wild10Center for Thrombosis and Hemostasis (CTH), University Medical Center Mainz, 55131 Mainz, GermanyCenter for Thrombosis and Hemostasis (CTH), University Medical Center Mainz, 55131 Mainz, GermanyCenter for Thrombosis and Hemostasis (CTH), University Medical Center Mainz, 55131 Mainz, GermanyCenter for Thrombosis and Hemostasis (CTH), University Medical Center Mainz, 55131 Mainz, GermanyCenter for Thrombosis and Hemostasis (CTH), University Medical Center Mainz, 55131 Mainz, GermanyCenter for Thrombosis and Hemostasis (CTH), University Medical Center Mainz, 55131 Mainz, GermanyCenter for Cardiology—Cardiology I, University Medical Center Mainz, 55131 Mainz, GermanyInstitute of Molecular Medicine, University Medical Center Mainz, 55131 Mainz, GermanyCenter for Thrombosis and Hemostasis (CTH), University Medical Center Mainz, 55131 Mainz, GermanyCenter for Thrombosis and Hemostasis (CTH), University Medical Center Mainz, 55131 Mainz, GermanyCenter for Thrombosis and Hemostasis (CTH), University Medical Center Mainz, 55131 Mainz, GermanyBackground: Psoriasis is a systemic inflammatory disorder, primarily characterized by skin plaques. It is linked to co-morbidities including cardiovascular disease and metabolic syndrome. Several studies demonstrate that dietary habits can influence psoriasis development and severity. However, the effect of different dietary protein levels on psoriasis development and severity is poorly understood. In this study, we examine the influence of dietary protein on psoriasis-like skin disease in mice. Methods: We fed male C57BL/6J mice with regular, low protein and high protein chow for 4 weeks. Afterwards, we induced psoriasis-like skin disease by topical imiquimod (IMQ)-treatment on ear and back skin. The local cutaneous and systemic inflammatory response was investigated using flow cytometry analysis, histology and quantitative rt-PCR. Results: After 5 days of IMQ-treatment, both diets reduced bodyweight in mice, whereas only the high protein diet slightly aggravated IMQ-induced skin inflammation. IMQ-treatment induced infiltration of myeloid cells, neutrophils, and monocytes/macrophages into skin and spleen independently of diet. After IMQ-treatment, circulating neutrophils and reactive oxygen species were increased in mice on low and high protein diets. Conclusion: Different dietary protein levels had no striking effect on IMQ-induced psoriasis but aggravated the systemic pro-inflammatory phenotype.https://www.mdpi.com/2072-6643/13/6/1897protein dietpsoriasisinflammationpsoriasis-like skin diseaseimiquimod |
| spellingShingle | Tanja Knopp Tabea Bieler Rebecca Jung Julia Ringen Michael Molitor Annika Jurda Thomas Münzel Ari Waisman Philip Wenzel Susanne Helena Karbach Johannes Wild Effects of Dietary Protein Intake on Cutaneous and Systemic Inflammation in Mice with Acute Experimental Psoriasis Nutrients protein diet psoriasis inflammation psoriasis-like skin disease imiquimod |
| title | Effects of Dietary Protein Intake on Cutaneous and Systemic Inflammation in Mice with Acute Experimental Psoriasis |
| title_full | Effects of Dietary Protein Intake on Cutaneous and Systemic Inflammation in Mice with Acute Experimental Psoriasis |
| title_fullStr | Effects of Dietary Protein Intake on Cutaneous and Systemic Inflammation in Mice with Acute Experimental Psoriasis |
| title_full_unstemmed | Effects of Dietary Protein Intake on Cutaneous and Systemic Inflammation in Mice with Acute Experimental Psoriasis |
| title_short | Effects of Dietary Protein Intake on Cutaneous and Systemic Inflammation in Mice with Acute Experimental Psoriasis |
| title_sort | effects of dietary protein intake on cutaneous and systemic inflammation in mice with acute experimental psoriasis |
| topic | protein diet psoriasis inflammation psoriasis-like skin disease imiquimod |
| url | https://www.mdpi.com/2072-6643/13/6/1897 |
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