Antibiotic-Resistant <i>Desulfovibrio</i> Produces H<sub>2</sub>S from Supplements for Animal Farming
Sulphate-reducing bacteria, primarily <i>Desulfovibrio</i>, are responsible for the active generation of H<sub>2</sub>S in swine production waste. The model species for sulphate reduction studies, <i>Desulfovibrio vulgaris</i> strain L2, was previously isolated fr...
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MDPI AG
2023-03-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/11/4/838 |
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| author | Olga V. Karnachuk Alexey V. Beletsky Andrey L. Rakitin Olga P. Ikkert Marat R. Avakyan Viacheslav S. Zyusman Andrei Napilov Andrey V. Mardanov Nikolai V. Ravin |
| author_facet | Olga V. Karnachuk Alexey V. Beletsky Andrey L. Rakitin Olga P. Ikkert Marat R. Avakyan Viacheslav S. Zyusman Andrei Napilov Andrey V. Mardanov Nikolai V. Ravin |
| author_sort | Olga V. Karnachuk |
| collection | DOAJ |
| description | Sulphate-reducing bacteria, primarily <i>Desulfovibrio</i>, are responsible for the active generation of H<sub>2</sub>S in swine production waste. The model species for sulphate reduction studies, <i>Desulfovibrio vulgaris</i> strain L2, was previously isolated from swine manure characterized by high rates of dissimilatory sulphate reduction. The source of electron acceptors in low-sulphate swine waste for the high rate of H<sub>2</sub>S formation remains uncertain. Here, we demonstrate the ability of the L2 strain to use common animal farming supplements including L-lysine-sulphate, gypsum and gypsum plasterboards as electron acceptors for H<sub>2</sub>S production. Genome sequencing of strain L2 revealed the presence of two megaplasmids and predicted resistance to various antimicrobials and mercury, which was confirmed in physiological experiments. Most of antibiotic resistance genes (ARG) are carried by two class 1 integrons located on the chromosome and on the plasmid pDsulf-L2-2. These ARGs, predicted to confer resistance to beta-lactams, aminoglycosides, lincosamides, sulphonamides, chloramphenicol and tetracycline, were probably laterally acquired from various <i>Gammaproteobacteria</i> and <i>Firmicutes</i>. Resistance to mercury is likely enabled by two <i>mer</i> operons also located on the chromosome and on pDsulf-L2-2 and acquired via horizontal gene transfer. The second megaplasmid, pDsulf-L2-1, encoded nitrogenase, catalase and type III secretion system suggesting close contact of the strain with intestinal cells in the swine gut. The location of ARGs on mobile elements allows us to consider <i>D. vulgaris</i> strain L2 as a possible vector transferring antimicrobials resistance determinants between the gut microbiote and microbial communities in environmental biotopes. |
| first_indexed | 2024-03-11T04:43:33Z |
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| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-11T04:43:33Z |
| publishDate | 2023-03-01 |
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| record_format | Article |
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| spelling | doaj.art-6ce9301adb134d159c111f70c3191a002023-11-17T20:31:16ZengMDPI AGMicroorganisms2076-26072023-03-0111483810.3390/microorganisms11040838Antibiotic-Resistant <i>Desulfovibrio</i> Produces H<sub>2</sub>S from Supplements for Animal FarmingOlga V. Karnachuk0Alexey V. Beletsky1Andrey L. Rakitin2Olga P. Ikkert3Marat R. Avakyan4Viacheslav S. Zyusman5Andrei Napilov6Andrey V. Mardanov7Nikolai V. Ravin8Laboratory of Biochemistry and Molecular Biology, Tomsk State University, 634050 Tomsk, RussiaInstitute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, Leninsky Prosp, Bld. 33-2, 119071 Moscow, RussiaInstitute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, Leninsky Prosp, Bld. 33-2, 119071 Moscow, RussiaLaboratory of Biochemistry and Molecular Biology, Tomsk State University, 634050 Tomsk, RussiaLaboratory of Biochemistry and Molecular Biology, Tomsk State University, 634050 Tomsk, RussiaLaboratory of Biochemistry and Molecular Biology, Tomsk State University, 634050 Tomsk, RussiaLaboratory of Biochemistry and Molecular Biology, Tomsk State University, 634050 Tomsk, RussiaInstitute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, Leninsky Prosp, Bld. 33-2, 119071 Moscow, RussiaInstitute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, Leninsky Prosp, Bld. 33-2, 119071 Moscow, RussiaSulphate-reducing bacteria, primarily <i>Desulfovibrio</i>, are responsible for the active generation of H<sub>2</sub>S in swine production waste. The model species for sulphate reduction studies, <i>Desulfovibrio vulgaris</i> strain L2, was previously isolated from swine manure characterized by high rates of dissimilatory sulphate reduction. The source of electron acceptors in low-sulphate swine waste for the high rate of H<sub>2</sub>S formation remains uncertain. Here, we demonstrate the ability of the L2 strain to use common animal farming supplements including L-lysine-sulphate, gypsum and gypsum plasterboards as electron acceptors for H<sub>2</sub>S production. Genome sequencing of strain L2 revealed the presence of two megaplasmids and predicted resistance to various antimicrobials and mercury, which was confirmed in physiological experiments. Most of antibiotic resistance genes (ARG) are carried by two class 1 integrons located on the chromosome and on the plasmid pDsulf-L2-2. These ARGs, predicted to confer resistance to beta-lactams, aminoglycosides, lincosamides, sulphonamides, chloramphenicol and tetracycline, were probably laterally acquired from various <i>Gammaproteobacteria</i> and <i>Firmicutes</i>. Resistance to mercury is likely enabled by two <i>mer</i> operons also located on the chromosome and on pDsulf-L2-2 and acquired via horizontal gene transfer. The second megaplasmid, pDsulf-L2-1, encoded nitrogenase, catalase and type III secretion system suggesting close contact of the strain with intestinal cells in the swine gut. The location of ARGs on mobile elements allows us to consider <i>D. vulgaris</i> strain L2 as a possible vector transferring antimicrobials resistance determinants between the gut microbiote and microbial communities in environmental biotopes.https://www.mdpi.com/2076-2607/11/4/838antibiotic resistanceresistance plasmidssulfate-reductionswine manuregut microbiota<i>Desulfovibrio</i> |
| spellingShingle | Olga V. Karnachuk Alexey V. Beletsky Andrey L. Rakitin Olga P. Ikkert Marat R. Avakyan Viacheslav S. Zyusman Andrei Napilov Andrey V. Mardanov Nikolai V. Ravin Antibiotic-Resistant <i>Desulfovibrio</i> Produces H<sub>2</sub>S from Supplements for Animal Farming Microorganisms antibiotic resistance resistance plasmids sulfate-reduction swine manure gut microbiota <i>Desulfovibrio</i> |
| title | Antibiotic-Resistant <i>Desulfovibrio</i> Produces H<sub>2</sub>S from Supplements for Animal Farming |
| title_full | Antibiotic-Resistant <i>Desulfovibrio</i> Produces H<sub>2</sub>S from Supplements for Animal Farming |
| title_fullStr | Antibiotic-Resistant <i>Desulfovibrio</i> Produces H<sub>2</sub>S from Supplements for Animal Farming |
| title_full_unstemmed | Antibiotic-Resistant <i>Desulfovibrio</i> Produces H<sub>2</sub>S from Supplements for Animal Farming |
| title_short | Antibiotic-Resistant <i>Desulfovibrio</i> Produces H<sub>2</sub>S from Supplements for Animal Farming |
| title_sort | antibiotic resistant i desulfovibrio i produces h sub 2 sub s from supplements for animal farming |
| topic | antibiotic resistance resistance plasmids sulfate-reduction swine manure gut microbiota <i>Desulfovibrio</i> |
| url | https://www.mdpi.com/2076-2607/11/4/838 |
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