The Relationship between Mastitis and Antimicrobial Peptide S100A7 Expression in Dairy Goats
S100A7 is an inflammation-related protein and plays an essential role in host defenses, yet there is little research about the relationship between mastitis and S100A7 expression in dairy goats. Here, according to the clinical diagnosis of udders, SCC, and bacteriological culture (BC) of milk, 84 da...
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| Format: | Article |
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MDPI AG
2023-11-01
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| Series: | Veterinary Sciences |
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| Online Access: | https://www.mdpi.com/2306-7381/10/11/653 |
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| author | Yutong Yan Kunyuan Zhu Haokun Liu Mingzhen Fan Xiaoe Zhao Menghao Pan Baohua Ma Qiang Wei |
| author_facet | Yutong Yan Kunyuan Zhu Haokun Liu Mingzhen Fan Xiaoe Zhao Menghao Pan Baohua Ma Qiang Wei |
| author_sort | Yutong Yan |
| collection | DOAJ |
| description | S100A7 is an inflammation-related protein and plays an essential role in host defenses, yet there is little research about the relationship between mastitis and S100A7 expression in dairy goats. Here, according to the clinical diagnosis of udders, SCC, and bacteriological culture (BC) of milk, 84 dairy goats were grouped into healthy goats (<i>n</i> = 25), subclinical mastitis goats (<i>n</i> = 36), and clinical mastitis goats (<i>n</i> = 23). The S100A7 concentration in subclinical mastitis goats was significantly upregulated than in healthy dairy goats (<i>p</i> = 0.0056) and had a limited change with clinical mastitis dairy goats (<i>p</i> = 0.8222). The relationship between log<sub>10</sub> SCC and S100A7 concentration in milk was positive and R = 0.05249; the regression equation was Y = 0.1446 × X + 12.54. According to the three groups, the log<sub>10</sub> SCC and S100A7 were analyzed using the receiver operating characteristics (ROC) curve; in subclinical mastitis goats, the area under the ROC curve (AUC) of log<sub>10</sub> SCC was 0.9222 and <i>p</i> < 0.0001, and the AUC of S100A7 concentration was 0.7317 and <i>p</i> = 0.0022, respectively; in clinical mastitis goats, the AUC of log<sub>10</sub> SCC was 0.9678 and <i>p</i> < 0.0001, and the AUC of S100A7 concentration was 0.5487 and <i>p</i> = 0.5634, respectively. In healthy goats, S100A7 was expressed weakly in the alveolus of the mammary gland of healthy goats while expressed densely in the collapsed alveolus of mastitis goats. Moreover, <i>S100A7</i> expression increased significantly in mastitis goats than in healthy dairy goats. In this research, results showed the effects of mastitis on the S100A7 expression in the mammary gland and S100A7 concentration in milk and the limited relationship between SCC and mastitis, which provided a new insight into S100A7’s role in the host defenses of dairy goats. |
| first_indexed | 2024-03-09T16:23:38Z |
| format | Article |
| id | doaj.art-6cea2152d85049bd977d1e6d2854bf76 |
| institution | Directory Open Access Journal |
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| spelling | doaj.art-6cea2152d85049bd977d1e6d2854bf762023-11-24T15:10:28ZengMDPI AGVeterinary Sciences2306-73812023-11-01101165310.3390/vetsci10110653The Relationship between Mastitis and Antimicrobial Peptide S100A7 Expression in Dairy GoatsYutong Yan0Kunyuan Zhu1Haokun Liu2Mingzhen Fan3Xiaoe Zhao4Menghao Pan5Baohua Ma6Qiang Wei7Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, Northwest A&F University, Xianyang 712100, ChinaKey Laboratory of Animal Biotechnology of the Ministry of Agriculture, Northwest A&F University, Xianyang 712100, ChinaKey Laboratory of Animal Biotechnology of the Ministry of Agriculture, Northwest A&F University, Xianyang 712100, ChinaKey Laboratory of Animal Biotechnology of the Ministry of Agriculture, Northwest A&F University, Xianyang 712100, ChinaKey Laboratory of Animal Biotechnology of the Ministry of Agriculture, Northwest A&F University, Xianyang 712100, ChinaKey Laboratory of Animal Biotechnology of the Ministry of Agriculture, Northwest A&F University, Xianyang 712100, ChinaKey Laboratory of Animal Biotechnology of the Ministry of Agriculture, Northwest A&F University, Xianyang 712100, ChinaKey Laboratory of Animal Biotechnology of the Ministry of Agriculture, Northwest A&F University, Xianyang 712100, ChinaS100A7 is an inflammation-related protein and plays an essential role in host defenses, yet there is little research about the relationship between mastitis and S100A7 expression in dairy goats. Here, according to the clinical diagnosis of udders, SCC, and bacteriological culture (BC) of milk, 84 dairy goats were grouped into healthy goats (<i>n</i> = 25), subclinical mastitis goats (<i>n</i> = 36), and clinical mastitis goats (<i>n</i> = 23). The S100A7 concentration in subclinical mastitis goats was significantly upregulated than in healthy dairy goats (<i>p</i> = 0.0056) and had a limited change with clinical mastitis dairy goats (<i>p</i> = 0.8222). The relationship between log<sub>10</sub> SCC and S100A7 concentration in milk was positive and R = 0.05249; the regression equation was Y = 0.1446 × X + 12.54. According to the three groups, the log<sub>10</sub> SCC and S100A7 were analyzed using the receiver operating characteristics (ROC) curve; in subclinical mastitis goats, the area under the ROC curve (AUC) of log<sub>10</sub> SCC was 0.9222 and <i>p</i> < 0.0001, and the AUC of S100A7 concentration was 0.7317 and <i>p</i> = 0.0022, respectively; in clinical mastitis goats, the AUC of log<sub>10</sub> SCC was 0.9678 and <i>p</i> < 0.0001, and the AUC of S100A7 concentration was 0.5487 and <i>p</i> = 0.5634, respectively. In healthy goats, S100A7 was expressed weakly in the alveolus of the mammary gland of healthy goats while expressed densely in the collapsed alveolus of mastitis goats. Moreover, <i>S100A7</i> expression increased significantly in mastitis goats than in healthy dairy goats. In this research, results showed the effects of mastitis on the S100A7 expression in the mammary gland and S100A7 concentration in milk and the limited relationship between SCC and mastitis, which provided a new insight into S100A7’s role in the host defenses of dairy goats.https://www.mdpi.com/2306-7381/10/11/653S100A7somatic cell countdairy goatmastitis |
| spellingShingle | Yutong Yan Kunyuan Zhu Haokun Liu Mingzhen Fan Xiaoe Zhao Menghao Pan Baohua Ma Qiang Wei The Relationship between Mastitis and Antimicrobial Peptide S100A7 Expression in Dairy Goats Veterinary Sciences S100A7 somatic cell count dairy goat mastitis |
| title | The Relationship between Mastitis and Antimicrobial Peptide S100A7 Expression in Dairy Goats |
| title_full | The Relationship between Mastitis and Antimicrobial Peptide S100A7 Expression in Dairy Goats |
| title_fullStr | The Relationship between Mastitis and Antimicrobial Peptide S100A7 Expression in Dairy Goats |
| title_full_unstemmed | The Relationship between Mastitis and Antimicrobial Peptide S100A7 Expression in Dairy Goats |
| title_short | The Relationship between Mastitis and Antimicrobial Peptide S100A7 Expression in Dairy Goats |
| title_sort | relationship between mastitis and antimicrobial peptide s100a7 expression in dairy goats |
| topic | S100A7 somatic cell count dairy goat mastitis |
| url | https://www.mdpi.com/2306-7381/10/11/653 |
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