Long-Read Sequencing and <i>De Novo</i> Genome Assembly Pipeline of Two <i>Plasmodium falciparum</i> Clones (<i>Pf</i>3D7, <i>Pf</i>W2) Using Only the PromethION Sequencer from Oxford Nanopore Technologies without Whole-Genome Amplification

Long-Read Sequencing and <i>De Novo</i> Genome Assembly Pipeline of Two <i>Plasmodium falciparum</i> Clones (<i>Pf</i>3D7, <i>Pf</i>W2) Using Only the PromethION Sequencer from Oxford Nanopore Technologies without Whole-Genome Amplification

Antimalarial drug resistance has become a real public health problem despite WHO measures. New sequencing technologies make it possible to investigate genomic variations associated with resistant phenotypes at the genome-wide scale. Based on the use of hemisynthetic nanopores, the PromethION technol...

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Bibliographic Details
Main Authors: Océane Delandre, Ombeline Lamer, Jean-Marie Loreau, Nasserdine Papa Mze, Isabelle Fonta, Joel Mosnier, Nicolas Gomez, Emilie Javelle, Bruno Pradines
Format: Article
Language:English
Published: MDPI AG 2024-01-01
Series:Biology
Subjects:
genome assembly
<i>Plasmodium falciparum</i>
long-read sequencing
nanopore
PromethION
<i>Pf</i>3D7
Online Access:https://www.mdpi.com/2079-7737/13/2/89

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