CHEMICAL MUTAGENESIS OF THE LYSINE-PRODUCING STRAIN Brevibacterium sp. IMV B-7447
The aim of the work was to obtain a producer strain with increased lysine accumulation using the chemical mutagenesis method. Methods. To achieve the goal, we used the method of treating the lysine-producing strain with the chemical mutagen N-methyl-N-nitro-N-nitrosoguanidine, cultivating the res...
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| Format: | Article |
| Language: | English |
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National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry
2022-06-01
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| Series: | Biotechnologia Acta |
| Subjects: | |
| Online Access: | http://biotechnology.kiev.ua/images/3_2022/andriiash_3_2022.pdf |
| _version_ | 1797705390027177984 |
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| author | Andriiash G. S. Beiko N. E. Tigunova O. O Shulga S. M. |
| author_facet | Andriiash G. S. Beiko N. E. Tigunova O. O Shulga S. M. |
| author_sort | Andriiash G. S. |
| collection | DOAJ |
| description | The aim of the work was to obtain a producer strain with increased lysine accumulation using the chemical mutagenesis method.
Methods. To achieve the goal, we used the method of treating the lysine-producing strain with the chemical mutagen N-methyl-N-nitro-N-nitrosoguanidine, cultivating the resulting clone and determining the accumulation of lysine in flasks and a bioreactor.
Results. The optimal concentrations and duration of mutagen action for the production of mutant microorganisms were found. Сlones with the maximum lysine accumulation were selected. Mutagenesis was carried out consecutively three times. As a result, lysine-producing strain Brevibacterium sp. IMV B-7796 auxotrophic regarding leucine and threonine with maximum accumulation of the target amino acid was obtained.
Conclusions. The lysine producer Brevibacterium sp. IMV B-7796 was obtained, which produced 65.0 g/dm3 of lysine in a bioreactor under conditions of periodic cultivation with feeding. The Brevibacterium sp. IMV B-7796 strain was proposed as a basis for the creation of a genetically modified strain with increased accumulation of lysine for further use in industrial lysine technology. |
| first_indexed | 2024-03-12T05:35:29Z |
| format | Article |
| id | doaj.art-6cf1dce0a1fd4ba7a8022a1eb3db6b3b |
| institution | Directory Open Access Journal |
| issn | 2410-7751 2410-776X |
| language | English |
| last_indexed | 2024-03-12T05:35:29Z |
| publishDate | 2022-06-01 |
| publisher | National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry |
| record_format | Article |
| series | Biotechnologia Acta |
| spelling | doaj.art-6cf1dce0a1fd4ba7a8022a1eb3db6b3b2023-09-03T06:27:30ZengNational Academy of Sciences of Ukraine, Palladin Institute of BiochemistryBiotechnologia Acta2410-77512410-776X2022-06-01153354110.15407/biotech15.03.035CHEMICAL MUTAGENESIS OF THE LYSINE-PRODUCING STRAIN Brevibacterium sp. IMV B-7447Andriiash G. S.0Beiko N. E.1 Tigunova O. O2Shulga S. M.3State Enterprise “Institute of Food Biotechnology and Genomics of the National Academy of Sciences of Ukraine”, KyivState Enterprise “Institute of Food Biotechnology and Genomics of the National Academy of Sciences of Ukraine”, KyivState Enterprise “Institute of Food Biotechnology and Genomics of the National Academy of Sciences of Ukraine”, KyivState Enterprise “Institute of Food Biotechnology and Genomics of the National Academy of Sciences of Ukraine”, KyivThe aim of the work was to obtain a producer strain with increased lysine accumulation using the chemical mutagenesis method. Methods. To achieve the goal, we used the method of treating the lysine-producing strain with the chemical mutagen N-methyl-N-nitro-N-nitrosoguanidine, cultivating the resulting clone and determining the accumulation of lysine in flasks and a bioreactor. Results. The optimal concentrations and duration of mutagen action for the production of mutant microorganisms were found. Сlones with the maximum lysine accumulation were selected. Mutagenesis was carried out consecutively three times. As a result, lysine-producing strain Brevibacterium sp. IMV B-7796 auxotrophic regarding leucine and threonine with maximum accumulation of the target amino acid was obtained. Conclusions. The lysine producer Brevibacterium sp. IMV B-7796 was obtained, which produced 65.0 g/dm3 of lysine in a bioreactor under conditions of periodic cultivation with feeding. The Brevibacterium sp. IMV B-7796 strain was proposed as a basis for the creation of a genetically modified strain with increased accumulation of lysine for further use in industrial lysine technology.http://biotechnology.kiev.ua/images/3_2022/andriiash_3_2022.pdfbrevibacterium sp.lysinechemical mutagenesisproducer strain. |
| spellingShingle | Andriiash G. S. Beiko N. E. Tigunova O. O Shulga S. M. CHEMICAL MUTAGENESIS OF THE LYSINE-PRODUCING STRAIN Brevibacterium sp. IMV B-7447 Biotechnologia Acta brevibacterium sp. lysine chemical mutagenesis producer strain. |
| title | CHEMICAL MUTAGENESIS OF THE LYSINE-PRODUCING STRAIN Brevibacterium sp. IMV B-7447 |
| title_full | CHEMICAL MUTAGENESIS OF THE LYSINE-PRODUCING STRAIN Brevibacterium sp. IMV B-7447 |
| title_fullStr | CHEMICAL MUTAGENESIS OF THE LYSINE-PRODUCING STRAIN Brevibacterium sp. IMV B-7447 |
| title_full_unstemmed | CHEMICAL MUTAGENESIS OF THE LYSINE-PRODUCING STRAIN Brevibacterium sp. IMV B-7447 |
| title_short | CHEMICAL MUTAGENESIS OF THE LYSINE-PRODUCING STRAIN Brevibacterium sp. IMV B-7447 |
| title_sort | chemical mutagenesis of the lysine producing strain brevibacterium sp imv b 7447 |
| topic | brevibacterium sp. lysine chemical mutagenesis producer strain. |
| url | http://biotechnology.kiev.ua/images/3_2022/andriiash_3_2022.pdf |
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