Towards safer non-volatile tissue fixatives: Evaluation of choline-based ionic liquids for fixing ocular tissues
Volatile organic chemicals (VOCs) are routinely used for processing biological tissue samples in clinical laboratories. Recognizing their serious health and environmental impacts, a few non-volatile green solvents (choline based ionic liquids, ILs) were evaluated as tissue fixatives here. Microscopi...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-02-01
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| Series: | Ecotoxicology and Environmental Safety |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651320316134 |
| _version_ | 1818731258920828928 |
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| author | P.R. Deepa V. Nalini M. Surianarayanan S. Krishnakumar |
| author_facet | P.R. Deepa V. Nalini M. Surianarayanan S. Krishnakumar |
| author_sort | P.R. Deepa |
| collection | DOAJ |
| description | Volatile organic chemicals (VOCs) are routinely used for processing biological tissue samples in clinical laboratories. Recognizing their serious health and environmental impacts, a few non-volatile green solvents (choline based ionic liquids, ILs) were evaluated as tissue fixatives here. Microscopic evaluation of histo-morphology, fixation and staining quality, and macromolecular integrity (DNA and proteins) were assessed in human eye tissues (sclera, choroid, retinal layers and retinal pigmented epithelium, eyelid and orbit) after IL-fixation. Formalin-fixed tissues were used as standard reference. Microscopic examination revealed favorable histomorphology, tissue fixation and staining characteristics in most tissues immersed in ILs. Time taken to fix, and stability over a period of time (24 h, 48 h, 1 week, 1 month) was also recorded. Electrophoretic analysis revealed stability of cellular proteins and nucleic acids in IL-fixed scleral tissues. Heterogeneity in tissue fixation property relative to the type of ocular tissue, duration of fixation and storage, warrant further design and optimization of ILs to fix biological tissues. The simple cholinium salts based ILs tested here show favorable potential for tissue fixation application, and as an alternative approach to the use of VOCs, towards sustainable biomedical practice. |
| first_indexed | 2024-12-17T23:14:50Z |
| format | Article |
| id | doaj.art-6cf28f86beb54e839490c50b9f008eb6 |
| institution | Directory Open Access Journal |
| issn | 0147-6513 |
| language | English |
| last_indexed | 2024-12-17T23:14:50Z |
| publishDate | 2021-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj.art-6cf28f86beb54e839490c50b9f008eb62022-12-21T21:29:02ZengElsevierEcotoxicology and Environmental Safety0147-65132021-02-01209111777Towards safer non-volatile tissue fixatives: Evaluation of choline-based ionic liquids for fixing ocular tissuesP.R. Deepa0V. Nalini1M. Surianarayanan2S. Krishnakumar3Department of Biological Sciences, Birla Institute of Technology & Science (BITS), Pilani 333 031, Rajasthan, India; Corresponding authors.Department of Biological Sciences, Birla Institute of Technology & Science (BITS), Pilani 333 031, Rajasthan, India; L&T Department of Ocular Pathology, Kamalnayan Bajaj Institute for Research in Vision and Ophthalmology, Vision Research Foundation, Sankara Nethralaya, 18, College Road, 600 006 Chennai, IndiaCell for Industrial Safety and Risk Analysis (CISRA), Central Leather Research Institute (CSIR-CLRI), Adyar, Chennai, IndiaL&T Department of Ocular Pathology, Kamalnayan Bajaj Institute for Research in Vision and Ophthalmology, Vision Research Foundation, Sankara Nethralaya, 18, College Road, 600 006 Chennai, India; Corresponding authors.Volatile organic chemicals (VOCs) are routinely used for processing biological tissue samples in clinical laboratories. Recognizing their serious health and environmental impacts, a few non-volatile green solvents (choline based ionic liquids, ILs) were evaluated as tissue fixatives here. Microscopic evaluation of histo-morphology, fixation and staining quality, and macromolecular integrity (DNA and proteins) were assessed in human eye tissues (sclera, choroid, retinal layers and retinal pigmented epithelium, eyelid and orbit) after IL-fixation. Formalin-fixed tissues were used as standard reference. Microscopic examination revealed favorable histomorphology, tissue fixation and staining characteristics in most tissues immersed in ILs. Time taken to fix, and stability over a period of time (24 h, 48 h, 1 week, 1 month) was also recorded. Electrophoretic analysis revealed stability of cellular proteins and nucleic acids in IL-fixed scleral tissues. Heterogeneity in tissue fixation property relative to the type of ocular tissue, duration of fixation and storage, warrant further design and optimization of ILs to fix biological tissues. The simple cholinium salts based ILs tested here show favorable potential for tissue fixation application, and as an alternative approach to the use of VOCs, towards sustainable biomedical practice.http://www.sciencedirect.com/science/article/pii/S0147651320316134FixativeHistomorphologyGreen solventsIonic liquidsOcular tissueSolvents effects |
| spellingShingle | P.R. Deepa V. Nalini M. Surianarayanan S. Krishnakumar Towards safer non-volatile tissue fixatives: Evaluation of choline-based ionic liquids for fixing ocular tissues Ecotoxicology and Environmental Safety Fixative Histomorphology Green solvents Ionic liquids Ocular tissue Solvents effects |
| title | Towards safer non-volatile tissue fixatives: Evaluation of choline-based ionic liquids for fixing ocular tissues |
| title_full | Towards safer non-volatile tissue fixatives: Evaluation of choline-based ionic liquids for fixing ocular tissues |
| title_fullStr | Towards safer non-volatile tissue fixatives: Evaluation of choline-based ionic liquids for fixing ocular tissues |
| title_full_unstemmed | Towards safer non-volatile tissue fixatives: Evaluation of choline-based ionic liquids for fixing ocular tissues |
| title_short | Towards safer non-volatile tissue fixatives: Evaluation of choline-based ionic liquids for fixing ocular tissues |
| title_sort | towards safer non volatile tissue fixatives evaluation of choline based ionic liquids for fixing ocular tissues |
| topic | Fixative Histomorphology Green solvents Ionic liquids Ocular tissue Solvents effects |
| url | http://www.sciencedirect.com/science/article/pii/S0147651320316134 |
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