Group I Intron Internal Guide Sequence Binding Strength as a Component of Ribozyme Network Formation
Origins-of-life research requires searching for a plausible transition from simple chemicals to larger macromolecules that can both hold information and catalyze their own production. We have previously shown that some group I intron ribozymes possess the ability to help synthesize other ribozyme ge...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2016-09-01
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| Series: | Molecules |
| Subjects: | |
| Online Access: | http://www.mdpi.com/1420-3049/21/10/1293 |
| _version_ | 1818480113101045760 |
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| author | Laura Elizabeth Satterwhite Jessica A. M. Yeates Niles Lehman |
| author_facet | Laura Elizabeth Satterwhite Jessica A. M. Yeates Niles Lehman |
| author_sort | Laura Elizabeth Satterwhite |
| collection | DOAJ |
| description | Origins-of-life research requires searching for a plausible transition from simple chemicals to larger macromolecules that can both hold information and catalyze their own production. We have previously shown that some group I intron ribozymes possess the ability to help synthesize other ribozyme genotypes by recombination reactions in small networks in an autocatalytic fashion. By simplifying these recombination reactions, using fluorescent anisotropy, we quantified the thermodynamic binding strength between two nucleotides of two group I intron RNA fragments for all 16 possible genotype combinations. We provide evidence that the binding strength (KD) between the 3-nucleotide internal guide sequence (IGS) of one ribozyme and its complement in another is correlated to the catalytic ability of the ribozyme. This work demonstrates that one can begin to deconstruct the thermodynamic basis of information in prebiotic RNA systems. |
| first_indexed | 2024-12-10T11:18:55Z |
| format | Article |
| id | doaj.art-6cf3c9d6104f43d59ff96d51611327ba |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-10T11:18:55Z |
| publishDate | 2016-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-6cf3c9d6104f43d59ff96d51611327ba2022-12-22T01:51:04ZengMDPI AGMolecules1420-30492016-09-012110129310.3390/molecules21101293molecules21101293Group I Intron Internal Guide Sequence Binding Strength as a Component of Ribozyme Network FormationLaura Elizabeth Satterwhite0Jessica A. M. Yeates1Niles Lehman2Department of Chemistry, Portland State University, Portland, OR 97202, USADepartment of Chemistry, Portland State University, Portland, OR 97202, USADepartment of Chemistry, Portland State University, Portland, OR 97202, USAOrigins-of-life research requires searching for a plausible transition from simple chemicals to larger macromolecules that can both hold information and catalyze their own production. We have previously shown that some group I intron ribozymes possess the ability to help synthesize other ribozyme genotypes by recombination reactions in small networks in an autocatalytic fashion. By simplifying these recombination reactions, using fluorescent anisotropy, we quantified the thermodynamic binding strength between two nucleotides of two group I intron RNA fragments for all 16 possible genotype combinations. We provide evidence that the binding strength (KD) between the 3-nucleotide internal guide sequence (IGS) of one ribozyme and its complement in another is correlated to the catalytic ability of the ribozyme. This work demonstrates that one can begin to deconstruct the thermodynamic basis of information in prebiotic RNA systems.http://www.mdpi.com/1420-3049/21/10/1293RNAribozymesorigins of lifeinternal guide sequencerecombinationfluorescence anisotropy |
| spellingShingle | Laura Elizabeth Satterwhite Jessica A. M. Yeates Niles Lehman Group I Intron Internal Guide Sequence Binding Strength as a Component of Ribozyme Network Formation Molecules RNA ribozymes origins of life internal guide sequence recombination fluorescence anisotropy |
| title | Group I Intron Internal Guide Sequence Binding Strength as a Component of Ribozyme Network Formation |
| title_full | Group I Intron Internal Guide Sequence Binding Strength as a Component of Ribozyme Network Formation |
| title_fullStr | Group I Intron Internal Guide Sequence Binding Strength as a Component of Ribozyme Network Formation |
| title_full_unstemmed | Group I Intron Internal Guide Sequence Binding Strength as a Component of Ribozyme Network Formation |
| title_short | Group I Intron Internal Guide Sequence Binding Strength as a Component of Ribozyme Network Formation |
| title_sort | group i intron internal guide sequence binding strength as a component of ribozyme network formation |
| topic | RNA ribozymes origins of life internal guide sequence recombination fluorescence anisotropy |
| url | http://www.mdpi.com/1420-3049/21/10/1293 |
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