Enhanced osteogenesis of quasi-three-dimensional hierarchical topography
Abstract Natural extracellular matrices (ECMs) are three-dimensional (3D) and multi-scale hierarchical structure. However, coatings used as ECM-mimicking structures for osteogenesis are typically two-dimensional or single-scaled. Here, we design a distinct quasi-three-dimensional hierarchical topogr...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2019-10-01
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| Series: | Journal of Nanobiotechnology |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12951-019-0536-5 |
| _version_ | 1828120698614185984 |
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| author | Mengfei Yu Yu Liu Xiaowen Yu Jianhua Li Wenquan Zhao Ji’an Hu Kui Cheng Wenjian Weng Bin Zhang Huiming Wang Lingqing Dong |
| author_facet | Mengfei Yu Yu Liu Xiaowen Yu Jianhua Li Wenquan Zhao Ji’an Hu Kui Cheng Wenjian Weng Bin Zhang Huiming Wang Lingqing Dong |
| author_sort | Mengfei Yu |
| collection | DOAJ |
| description | Abstract Natural extracellular matrices (ECMs) are three-dimensional (3D) and multi-scale hierarchical structure. However, coatings used as ECM-mimicking structures for osteogenesis are typically two-dimensional or single-scaled. Here, we design a distinct quasi-three-dimensional hierarchical topography integrated of density-controlled titania nanodots and nanorods. We find cellular pseudopods preferred to anchor deeply across the distinct 3D topography, dependently of the relative density of nanorods, which promote the osteogenic differentiation of osteoblast but not the viability of fibroblast. The in vivo experimental results further indicate that the new bone formation, the relative bone-implant contact as well as the push-put strength, are significantly enhanced on the 3D hierarchical topography. We also show that the exposures of HFN7.1 and mAb1937 critical functional motifs of fibronectin for cellular anchorage are up-regulated on the 3D hierarchical topography, which might synergistically promote the osteogenesis. Our findings suggest the multi-dimensions and multi-scales as vital characteristic of cell-ECM interactions and as an important design parameter for bone implant coatings. |
| first_indexed | 2024-04-11T14:10:16Z |
| format | Article |
| id | doaj.art-6cf40e0a0d08436aa7c9f68e7ebfe801 |
| institution | Directory Open Access Journal |
| issn | 1477-3155 |
| language | English |
| last_indexed | 2024-04-11T14:10:16Z |
| publishDate | 2019-10-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Nanobiotechnology |
| spelling | doaj.art-6cf40e0a0d08436aa7c9f68e7ebfe8012022-12-22T04:19:45ZengBMCJournal of Nanobiotechnology1477-31552019-10-0117111310.1186/s12951-019-0536-5Enhanced osteogenesis of quasi-three-dimensional hierarchical topographyMengfei Yu0Yu Liu1Xiaowen Yu2Jianhua Li3Wenquan Zhao4Ji’an Hu5Kui Cheng6Wenjian Weng7Bin Zhang8Huiming Wang9Lingqing Dong10The Affiliated Stomatologic Hospital, School of Medicine, Zhejiang UniversityThe Affiliated Stomatologic Hospital, School of Medicine, Zhejiang UniversityThe Affiliated Stomatologic Hospital, School of Medicine, Zhejiang UniversityHangzhou Dental HospitalThe Affiliated Stomatologic Hospital, School of Medicine, Zhejiang UniversityThe Affiliated Stomatologic Hospital, School of Medicine, Zhejiang UniversitySchool of Materials Science and Engineering, State Key Laboratory of Silicon Materials, Zhejiang UniversitySchool of Materials Science and Engineering, State Key Laboratory of Silicon Materials, Zhejiang UniversityThe State Key Laboratory of Fluid Power Transmission and Control, Zhejiang UniversityThe Affiliated Stomatologic Hospital, School of Medicine, Zhejiang UniversityThe Affiliated Stomatologic Hospital, School of Medicine, Zhejiang UniversityAbstract Natural extracellular matrices (ECMs) are three-dimensional (3D) and multi-scale hierarchical structure. However, coatings used as ECM-mimicking structures for osteogenesis are typically two-dimensional or single-scaled. Here, we design a distinct quasi-three-dimensional hierarchical topography integrated of density-controlled titania nanodots and nanorods. We find cellular pseudopods preferred to anchor deeply across the distinct 3D topography, dependently of the relative density of nanorods, which promote the osteogenic differentiation of osteoblast but not the viability of fibroblast. The in vivo experimental results further indicate that the new bone formation, the relative bone-implant contact as well as the push-put strength, are significantly enhanced on the 3D hierarchical topography. We also show that the exposures of HFN7.1 and mAb1937 critical functional motifs of fibronectin for cellular anchorage are up-regulated on the 3D hierarchical topography, which might synergistically promote the osteogenesis. Our findings suggest the multi-dimensions and multi-scales as vital characteristic of cell-ECM interactions and as an important design parameter for bone implant coatings.http://link.springer.com/article/10.1186/s12951-019-0536-5OsteogenesisQuasi-three-dimensionalHierarchicalNanorodsTitania |
| spellingShingle | Mengfei Yu Yu Liu Xiaowen Yu Jianhua Li Wenquan Zhao Ji’an Hu Kui Cheng Wenjian Weng Bin Zhang Huiming Wang Lingqing Dong Enhanced osteogenesis of quasi-three-dimensional hierarchical topography Journal of Nanobiotechnology Osteogenesis Quasi-three-dimensional Hierarchical Nanorods Titania |
| title | Enhanced osteogenesis of quasi-three-dimensional hierarchical topography |
| title_full | Enhanced osteogenesis of quasi-three-dimensional hierarchical topography |
| title_fullStr | Enhanced osteogenesis of quasi-three-dimensional hierarchical topography |
| title_full_unstemmed | Enhanced osteogenesis of quasi-three-dimensional hierarchical topography |
| title_short | Enhanced osteogenesis of quasi-three-dimensional hierarchical topography |
| title_sort | enhanced osteogenesis of quasi three dimensional hierarchical topography |
| topic | Osteogenesis Quasi-three-dimensional Hierarchical Nanorods Titania |
| url | http://link.springer.com/article/10.1186/s12951-019-0536-5 |
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