Anti-Influenza A Virus Activity of Rhamnan Sulfate from Green Algae <i>Monostroma nitidum</i> in Mice with Normal and Compromised Immunity
Influenza viruses cause a significant public health burden each year despite the availability of anti-influenza drugs and vaccines. Therefore, new anti-influenza virus agents are needed. Rhamnan sulfate (RS) is a sulfated polysaccharide derived from the green alga <i>Monostroma nitidum</i&g...
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MDPI AG
2020-05-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/18/5/254 |
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| author | Masahiro Terasawa Kyoko Hayashi Jung-Bum Lee Kaoru Nishiura Koichi Matsuda Toshimitsu Hayashi Toshio Kawahara |
| author_facet | Masahiro Terasawa Kyoko Hayashi Jung-Bum Lee Kaoru Nishiura Koichi Matsuda Toshimitsu Hayashi Toshio Kawahara |
| author_sort | Masahiro Terasawa |
| collection | DOAJ |
| description | Influenza viruses cause a significant public health burden each year despite the availability of anti-influenza drugs and vaccines. Therefore, new anti-influenza virus agents are needed. Rhamnan sulfate (RS) is a sulfated polysaccharide derived from the green alga <i>Monostroma nitidum</i>. Here, we aimed to demonstrate the antiviral activity of RS, especially against influenza A virus (IFV) infection, in vitro and in vivo. RS showed inhibitory effects on viral proliferation of enveloped viruses in vitro. Evaluation of the anti-IFV activity of RS in vitro showed that it inhibited both virus adsorption and entry steps. The oral administration of RS in IFV-infected immunocompetent and immunocompromised mice suppressed viral proliferation in both mouse types. The oral administration of RS also had stimulatory effects on neutralizing antibody production. Fluorescent analysis showed that RS colocalized with M cells in Peyer’s patches, suggesting that RS bound to the M cells and may be incorporated into the Peyer’s patches, which are essential to intestinal immunity. In summary, RS inhibits influenza virus infection and promotes antibody production, suggesting that RS is a potential candidate for the treatment of influenza virus infections. |
| first_indexed | 2024-03-10T19:51:33Z |
| format | Article |
| id | doaj.art-6cf702fc05dc4ae4aed7e658b207dc93 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-03-10T19:51:33Z |
| publishDate | 2020-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-6cf702fc05dc4ae4aed7e658b207dc932023-11-20T00:20:39ZengMDPI AGMarine Drugs1660-33972020-05-0118525410.3390/md18050254Anti-Influenza A Virus Activity of Rhamnan Sulfate from Green Algae <i>Monostroma nitidum</i> in Mice with Normal and Compromised ImmunityMasahiro Terasawa0Kyoko Hayashi1Jung-Bum Lee2Kaoru Nishiura3Koichi Matsuda4Toshimitsu Hayashi5Toshio Kawahara6Konan Chemical Manufacturing Co., LTD., 1515 Kitagomizuka, Kusu-cho, Yokkaichi, Mie 510-0103, JapanGraduate School of Engineering, Chubu University, 1200 Matsumoto-cho, Kasugai, Aichi 487-8501, JapanFaculty of Pharmaceutical Sciences, University of Toyama, 2630 Sugitani, Toyama, Toyama 930-0194, JapanKonan Chemical Manufacturing Co., LTD., 1515 Kitagomizuka, Kusu-cho, Yokkaichi, Mie 510-0103, JapanKonan Chemical Manufacturing Co., LTD., 1515 Kitagomizuka, Kusu-cho, Yokkaichi, Mie 510-0103, JapanCollege of Life and Health Sciences, Chubu University, 1200 Matsumoto-cho, Kasugai, Aichi 487-8501, JapanCollege of Life and Health Sciences, Chubu University, 1200 Matsumoto-cho, Kasugai, Aichi 487-8501, JapanInfluenza viruses cause a significant public health burden each year despite the availability of anti-influenza drugs and vaccines. Therefore, new anti-influenza virus agents are needed. Rhamnan sulfate (RS) is a sulfated polysaccharide derived from the green alga <i>Monostroma nitidum</i>. Here, we aimed to demonstrate the antiviral activity of RS, especially against influenza A virus (IFV) infection, in vitro and in vivo. RS showed inhibitory effects on viral proliferation of enveloped viruses in vitro. Evaluation of the anti-IFV activity of RS in vitro showed that it inhibited both virus adsorption and entry steps. The oral administration of RS in IFV-infected immunocompetent and immunocompromised mice suppressed viral proliferation in both mouse types. The oral administration of RS also had stimulatory effects on neutralizing antibody production. Fluorescent analysis showed that RS colocalized with M cells in Peyer’s patches, suggesting that RS bound to the M cells and may be incorporated into the Peyer’s patches, which are essential to intestinal immunity. In summary, RS inhibits influenza virus infection and promotes antibody production, suggesting that RS is a potential candidate for the treatment of influenza virus infections.https://www.mdpi.com/1660-3397/18/5/254antiviralinfluenza virusrhamnan sulfate |
| spellingShingle | Masahiro Terasawa Kyoko Hayashi Jung-Bum Lee Kaoru Nishiura Koichi Matsuda Toshimitsu Hayashi Toshio Kawahara Anti-Influenza A Virus Activity of Rhamnan Sulfate from Green Algae <i>Monostroma nitidum</i> in Mice with Normal and Compromised Immunity Marine Drugs antiviral influenza virus rhamnan sulfate |
| title | Anti-Influenza A Virus Activity of Rhamnan Sulfate from Green Algae <i>Monostroma nitidum</i> in Mice with Normal and Compromised Immunity |
| title_full | Anti-Influenza A Virus Activity of Rhamnan Sulfate from Green Algae <i>Monostroma nitidum</i> in Mice with Normal and Compromised Immunity |
| title_fullStr | Anti-Influenza A Virus Activity of Rhamnan Sulfate from Green Algae <i>Monostroma nitidum</i> in Mice with Normal and Compromised Immunity |
| title_full_unstemmed | Anti-Influenza A Virus Activity of Rhamnan Sulfate from Green Algae <i>Monostroma nitidum</i> in Mice with Normal and Compromised Immunity |
| title_short | Anti-Influenza A Virus Activity of Rhamnan Sulfate from Green Algae <i>Monostroma nitidum</i> in Mice with Normal and Compromised Immunity |
| title_sort | anti influenza a virus activity of rhamnan sulfate from green algae i monostroma nitidum i in mice with normal and compromised immunity |
| topic | antiviral influenza virus rhamnan sulfate |
| url | https://www.mdpi.com/1660-3397/18/5/254 |
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