SaMSTNb23 and SaMSTNb33: Emerging Markers for Growth Traits in Huchen (Hucho hucho, Linnaeus, 1758)
Huchen or Danube salmon (Hucho hucho, Linnaeus, 1758) is an economically and ecologically valuable salmonid species, among the most endangered fish species inhabiting the Danube basin. Consequently, appropriate conservation measures are required to prevent the species extinction. Although H. hucho i...
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| Format: | Article |
| Language: | English |
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Agroprint Timisoara
2023-09-01
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| Series: | Scientific Papers Animal Science and Biotechnologies |
| Subjects: | |
| Online Access: | https://spasb.ro/index.php/public_html/article/view/973 |
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| author | Gina–Oana Popa Ramona Nechifor Alexandru Burcea Maria Samu Andreea Dudu Marieta Costache Marilena Maereanu Sergiu Emil Georgescu |
| author_facet | Gina–Oana Popa Ramona Nechifor Alexandru Burcea Maria Samu Andreea Dudu Marieta Costache Marilena Maereanu Sergiu Emil Georgescu |
| author_sort | Gina–Oana Popa |
| collection | DOAJ |
| description | Huchen or Danube salmon (Hucho hucho, Linnaeus, 1758) is an economically and ecologically valuable salmonid species, among the most endangered fish species inhabiting the Danube basin. Consequently, appropriate conservation measures are required to prevent the species extinction. Although H. hucho is a poorly studied species, there are several studies on other salmonid species regarding fast growth markers. Following these studies, the main purpose of the current study was to determine growth-related SNPs in myostatin gene in H. hucho. Therefore, we analysed 20 aquaculture individuals of same age separated in two groups according length and weight, by sequencing the amplification product of SaMSTNb23 and SaMSTNb33 primers pairs. We also statistically analysed the differences in the development of individuals over time regarding total length, weight and head length. Significantly variations were observed (p < 0.001) for the measured traits in both groups. Regarding the genetic analysis, we observed nine SNPs in the mstn gene structure. Considering these results, it appears that the analysed markers are not appropriate for genetic analysis of growth traits in H. hucho. To extend gene expression pattern analysis, other markers are recommended for further evaluation. |
| first_indexed | 2024-04-24T11:29:53Z |
| format | Article |
| id | doaj.art-6cfbb503a3dc4170818ecb8d8b2fa42c |
| institution | Directory Open Access Journal |
| issn | 1841-9364 2344-4576 |
| language | English |
| last_indexed | 2024-04-24T11:29:53Z |
| publishDate | 2023-09-01 |
| publisher | Agroprint Timisoara |
| record_format | Article |
| series | Scientific Papers Animal Science and Biotechnologies |
| spelling | doaj.art-6cfbb503a3dc4170818ecb8d8b2fa42c2024-04-10T10:58:09ZengAgroprint TimisoaraScientific Papers Animal Science and Biotechnologies1841-93642344-45762023-09-015126161973SaMSTNb23 and SaMSTNb33: Emerging Markers for Growth Traits in Huchen (Hucho hucho, Linnaeus, 1758)Gina–Oana Popa0Ramona Nechifor1Alexandru Burcea2Maria Samu3Andreea Dudu4Marieta Costache5Marilena Maereanu6Sergiu Emil Georgescu7University of Bucharest, Faculty of Biology, Department of Biochemistry and Molecular Biology, 91-95 Splaiul Independenței, 050095, Bucharest, RomaniaUniversity of Bucharest, Faculty of Biology, Department of Biochemistry and Molecular Biology, 91-95 Splaiul Independenței, 050095, Bucharest, RomaniaUniversity of Bucharest, Faculty of Biology, Department of Biochemistry and Molecular Biology, 91-95 Splaiul Independenței, 050095, Bucharest, RomaniaUniversity of Bucharest, Faculty of Biology, Department of Biochemistry and Molecular Biology, 91-95 Splaiul Independenței, 050095, Bucharest, RomaniaUniversity of Bucharest, Faculty of Biology, Department of Biochemistry and Molecular Biology, 91-95 Splaiul Independenței, 050095, Bucharest, RomaniaUniversity of Bucharest, Faculty of Biology, Department of Biochemistry and Molecular Biology, 91-95 Splaiul Independenței, 050095, Bucharest, RomaniaS.C. Danube Research-Consulting S.R.L., 89bis Câmpia Libertății Street, 825200, Isaccea, Romania ‡The first two authors contributed equally to this work.University of Bucharest, Faculty of Biology, Department of Biochemistry and Molecular Biology, 91-95 Splaiul Independenței, 050095, Bucharest, RomaniaHuchen or Danube salmon (Hucho hucho, Linnaeus, 1758) is an economically and ecologically valuable salmonid species, among the most endangered fish species inhabiting the Danube basin. Consequently, appropriate conservation measures are required to prevent the species extinction. Although H. hucho is a poorly studied species, there are several studies on other salmonid species regarding fast growth markers. Following these studies, the main purpose of the current study was to determine growth-related SNPs in myostatin gene in H. hucho. Therefore, we analysed 20 aquaculture individuals of same age separated in two groups according length and weight, by sequencing the amplification product of SaMSTNb23 and SaMSTNb33 primers pairs. We also statistically analysed the differences in the development of individuals over time regarding total length, weight and head length. Significantly variations were observed (p < 0.001) for the measured traits in both groups. Regarding the genetic analysis, we observed nine SNPs in the mstn gene structure. Considering these results, it appears that the analysed markers are not appropriate for genetic analysis of growth traits in H. hucho. To extend gene expression pattern analysis, other markers are recommended for further evaluation.https://spasb.ro/index.php/public_html/article/view/973aquaculturegrowth traitshuchenmyostatinsingle nucleotide polymorphisms |
| spellingShingle | Gina–Oana Popa Ramona Nechifor Alexandru Burcea Maria Samu Andreea Dudu Marieta Costache Marilena Maereanu Sergiu Emil Georgescu SaMSTNb23 and SaMSTNb33: Emerging Markers for Growth Traits in Huchen (Hucho hucho, Linnaeus, 1758) Scientific Papers Animal Science and Biotechnologies aquaculture growth traits huchen myostatin single nucleotide polymorphisms |
| title | SaMSTNb23 and SaMSTNb33: Emerging Markers for Growth Traits in Huchen (Hucho hucho, Linnaeus, 1758) |
| title_full | SaMSTNb23 and SaMSTNb33: Emerging Markers for Growth Traits in Huchen (Hucho hucho, Linnaeus, 1758) |
| title_fullStr | SaMSTNb23 and SaMSTNb33: Emerging Markers for Growth Traits in Huchen (Hucho hucho, Linnaeus, 1758) |
| title_full_unstemmed | SaMSTNb23 and SaMSTNb33: Emerging Markers for Growth Traits in Huchen (Hucho hucho, Linnaeus, 1758) |
| title_short | SaMSTNb23 and SaMSTNb33: Emerging Markers for Growth Traits in Huchen (Hucho hucho, Linnaeus, 1758) |
| title_sort | samstnb23 and samstnb33 emerging markers for growth traits in huchen hucho hucho linnaeus 1758 |
| topic | aquaculture growth traits huchen myostatin single nucleotide polymorphisms |
| url | https://spasb.ro/index.php/public_html/article/view/973 |
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