Investigating of the microbial communities in a red circle disease of sea cucumber based on metagenomic sequencing
Diseases in aquaculture restrict the survival and development of Apostichopus japonicus, one of the most important Chinese marine culture species. A novel disease, named ''red circle'' disease, is frequently observed in sea cucumber seedling ponds and results in the death of sea...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-02-01
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| Series: | Aquaculture Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513422004410 |
| _version_ | 1797951518386683904 |
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| author | Zhen Zhang Yan Liu Ziyan Wang Zhonghua Wang Jun Zhou Jiaojiao Han Chenyang Lu Ye Li Tinghong Ming Xiurong Su |
| author_facet | Zhen Zhang Yan Liu Ziyan Wang Zhonghua Wang Jun Zhou Jiaojiao Han Chenyang Lu Ye Li Tinghong Ming Xiurong Su |
| author_sort | Zhen Zhang |
| collection | DOAJ |
| description | Diseases in aquaculture restrict the survival and development of Apostichopus japonicus, one of the most important Chinese marine culture species. A novel disease, named ''red circle'' disease, is frequently observed in sea cucumber seedling ponds and results in the death of sea cucumbers. No information about this disease in A. japonicus is available. In this study, we investigated the whole microbial community structure and predicted microbial functional profiles in ''red circle'' disease affecting sea cucumbers through metagenomics. Metataxonomic analysis of ''red circle'' samples showed different domains, namely, bacteria (71.39%), virus (5.11%), archaea (0.15%), eukaryotes (0.06%), and some unclassified microbiota (23.29%). Proteobacteria was the most abundant phylum (42.88%), followed by Bacteroidetes (3.30%), Viruses_noname (3.12%), Verrucomicrobia (2.99%), Planctomycetes (2.67%), and Viruses_unclassified (1.99%). The species belong to genus Vibrio, which accounted for 4.51% of the microbiota. Vibrio alginolyticus was the most abundant species of genus Vibrio in the ''red circle'' samples, followed by Vibrio diabolicus, Vibrio parahaemolyticus, Vibrio cholerae, and Vibrio tubiashii. Functional analysis of ''red circle'' samples metagenome revealed the genes for the metabolism and environmental information processing of a wide range of bioactive compounds, including amino acids and carbohydrates. Results of our study provides insight into the ''red circle'' disease and microbial community in A. japonicus seedling ponds and widens our understanding of this new disease. |
| first_indexed | 2024-04-10T22:32:52Z |
| format | Article |
| id | doaj.art-6cff3bae420d4a0b8b838f5fbff6c366 |
| institution | Directory Open Access Journal |
| issn | 2352-5134 |
| language | English |
| last_indexed | 2024-04-10T22:32:52Z |
| publishDate | 2023-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj.art-6cff3bae420d4a0b8b838f5fbff6c3662023-01-17T04:07:11ZengElsevierAquaculture Reports2352-51342023-02-0128101445Investigating of the microbial communities in a red circle disease of sea cucumber based on metagenomic sequencingZhen Zhang0Yan Liu1Ziyan Wang2Zhonghua Wang3Jun Zhou4Jiaojiao Han5Chenyang Lu6Ye Li7Tinghong Ming8Xiurong Su9State Key Laboratory for Quality and Safety of Argo-products, Ningbo University, Ningbo 315201, China; School of Marine Science, Ningbo University, Ningbo 315832, China; Key Laboratory of Aquacultal Biotechnology (Ningbo University) Ministry of Education, Ningbo University, Ningbo 315832, ChinaState Key Laboratory for Quality and Safety of Argo-products, Ningbo University, Ningbo 315201, China; School of Marine Science, Ningbo University, Ningbo 315832, China; Key Laboratory of Aquacultal Biotechnology (Ningbo University) Ministry of Education, Ningbo University, Ningbo 315832, ChinaState Key Laboratory for Quality and Safety of Argo-products, Ningbo University, Ningbo 315201, China; School of Marine Science, Ningbo University, Ningbo 315832, China; Key Laboratory of Aquacultal Biotechnology (Ningbo University) Ministry of Education, Ningbo University, Ningbo 315832, ChinaShandong Beiyou Biotechnology Co., Ltd, Weifang 261208, ChinaState Key Laboratory for Quality and Safety of Argo-products, Ningbo University, Ningbo 315201, China; School of Marine Science, Ningbo University, Ningbo 315832, China; Key Laboratory of Aquacultal Biotechnology (Ningbo University) Ministry of Education, Ningbo University, Ningbo 315832, ChinaState Key Laboratory for Quality and Safety of Argo-products, Ningbo University, Ningbo 315201, China; School of Marine Science, Ningbo University, Ningbo 315832, China; Key Laboratory of Aquacultal Biotechnology (Ningbo University) Ministry of Education, Ningbo University, Ningbo 315832, ChinaState Key Laboratory for Quality and Safety of Argo-products, Ningbo University, Ningbo 315201, China; School of Marine Science, Ningbo University, Ningbo 315832, China; Key Laboratory of Aquacultal Biotechnology (Ningbo University) Ministry of Education, Ningbo University, Ningbo 315832, ChinaState Key Laboratory for Quality and Safety of Argo-products, Ningbo University, Ningbo 315201, China; School of Marine Science, Ningbo University, Ningbo 315832, China; Key Laboratory of Aquacultal Biotechnology (Ningbo University) Ministry of Education, Ningbo University, Ningbo 315832, ChinaState Key Laboratory for Quality and Safety of Argo-products, Ningbo University, Ningbo 315201, China; School of Marine Science, Ningbo University, Ningbo 315832, China; Key Laboratory of Aquacultal Biotechnology (Ningbo University) Ministry of Education, Ningbo University, Ningbo 315832, ChinaState Key Laboratory for Quality and Safety of Argo-products, Ningbo University, Ningbo 315201, China; School of Marine Science, Ningbo University, Ningbo 315832, China; Key Laboratory of Aquacultal Biotechnology (Ningbo University) Ministry of Education, Ningbo University, Ningbo 315832, China; Corresponding author at: State Key Laboratory for Quality and Safety of Argo-products, Ningbo University, Ningbo 315201, China.Diseases in aquaculture restrict the survival and development of Apostichopus japonicus, one of the most important Chinese marine culture species. A novel disease, named ''red circle'' disease, is frequently observed in sea cucumber seedling ponds and results in the death of sea cucumbers. No information about this disease in A. japonicus is available. In this study, we investigated the whole microbial community structure and predicted microbial functional profiles in ''red circle'' disease affecting sea cucumbers through metagenomics. Metataxonomic analysis of ''red circle'' samples showed different domains, namely, bacteria (71.39%), virus (5.11%), archaea (0.15%), eukaryotes (0.06%), and some unclassified microbiota (23.29%). Proteobacteria was the most abundant phylum (42.88%), followed by Bacteroidetes (3.30%), Viruses_noname (3.12%), Verrucomicrobia (2.99%), Planctomycetes (2.67%), and Viruses_unclassified (1.99%). The species belong to genus Vibrio, which accounted for 4.51% of the microbiota. Vibrio alginolyticus was the most abundant species of genus Vibrio in the ''red circle'' samples, followed by Vibrio diabolicus, Vibrio parahaemolyticus, Vibrio cholerae, and Vibrio tubiashii. Functional analysis of ''red circle'' samples metagenome revealed the genes for the metabolism and environmental information processing of a wide range of bioactive compounds, including amino acids and carbohydrates. Results of our study provides insight into the ''red circle'' disease and microbial community in A. japonicus seedling ponds and widens our understanding of this new disease.http://www.sciencedirect.com/science/article/pii/S2352513422004410Apostichopus japonicusMetagenomics technologyMicrobial communities''red circle'' disease |
| spellingShingle | Zhen Zhang Yan Liu Ziyan Wang Zhonghua Wang Jun Zhou Jiaojiao Han Chenyang Lu Ye Li Tinghong Ming Xiurong Su Investigating of the microbial communities in a red circle disease of sea cucumber based on metagenomic sequencing Aquaculture Reports Apostichopus japonicus Metagenomics technology Microbial communities ''red circle'' disease |
| title | Investigating of the microbial communities in a red circle disease of sea cucumber based on metagenomic sequencing |
| title_full | Investigating of the microbial communities in a red circle disease of sea cucumber based on metagenomic sequencing |
| title_fullStr | Investigating of the microbial communities in a red circle disease of sea cucumber based on metagenomic sequencing |
| title_full_unstemmed | Investigating of the microbial communities in a red circle disease of sea cucumber based on metagenomic sequencing |
| title_short | Investigating of the microbial communities in a red circle disease of sea cucumber based on metagenomic sequencing |
| title_sort | investigating of the microbial communities in a red circle disease of sea cucumber based on metagenomic sequencing |
| topic | Apostichopus japonicus Metagenomics technology Microbial communities ''red circle'' disease |
| url | http://www.sciencedirect.com/science/article/pii/S2352513422004410 |
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