Collagen Membranes Functionalized with 150 Cycles of Atomic Layer Deposited Titania Improve Osteopromotive Property in Critical-Size Defects Created on Rat Calvaria
The membranes used in bone reconstructions have been the object of investigation in the field of tissue engineering, seeking to improve their mechanical strength and add other properties, mainly the osteopromotive. This study aimed to evaluate the functionalization of collagen membranes, with atomic...
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MDPI AG
2023-02-01
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| Series: | Journal of Functional Biomaterials |
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| Online Access: | https://www.mdpi.com/2079-4983/14/3/120 |
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| author | Leonardo P. Faverani Sarah Hashemi Astaneh Monique Gonçalves da Costa Leonardo A. Delanora Tiburtino J. Lima-Neto Stéfany Barbosa Maretaningtias Dwi Ariani Christos Takoudis Cortino Sukotjo |
| author_facet | Leonardo P. Faverani Sarah Hashemi Astaneh Monique Gonçalves da Costa Leonardo A. Delanora Tiburtino J. Lima-Neto Stéfany Barbosa Maretaningtias Dwi Ariani Christos Takoudis Cortino Sukotjo |
| author_sort | Leonardo P. Faverani |
| collection | DOAJ |
| description | The membranes used in bone reconstructions have been the object of investigation in the field of tissue engineering, seeking to improve their mechanical strength and add other properties, mainly the osteopromotive. This study aimed to evaluate the functionalization of collagen membranes, with atomic layer deposition of TiO<sub>2</sub> on the bone repair of critical defects in rat calvaria and subcutaneous biocompatibility. A total of 39 male rats were randomized into four groups: blood clot (BC), collagen membrane (COL), COL 150—150 cycles of titania, and COL 600—600 cycles of titania. The defects were created in each calvaria (5 mm in diameter) and covered according to each group; the animals were euthanized at 7, 14, and 28 days. The collected samples were assessed by histometric (newly bone formed, soft tissue area, membrane area, and residual linear defect) and histologic (inflammatory cells and blood cells count) analysis. All data were subjected to statistical analysis (<i>p</i> < 0.05). The COL150 group showed statistically significant differences compared to the other groups, mainly in the analysis of residual linear defects (1.5 ± 0.5 × 10<sup>6</sup> pixels/µm<sup>2</sup> for COL 150, and around 1 ± 0.5 × 10<sup>6</sup> pixels/µm<sup>2</sup> for the other groups) and newly formed bone (1500 ± 1200 pixels/µm for COL 150, and around 4000 pixels/µm for the others) (<i>p</i> < 0.05), demonstrating a better biological behavior in the chronology of defects repair. It is concluded that the collagen membrane functionalized by TiO<sub>2</sub> over 150 cycles showed better bioactive potential in treating critical size defects in the rats’ calvaria. |
| first_indexed | 2024-03-11T06:21:10Z |
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| institution | Directory Open Access Journal |
| issn | 2079-4983 |
| language | English |
| last_indexed | 2024-03-11T06:21:10Z |
| publishDate | 2023-02-01 |
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| series | Journal of Functional Biomaterials |
| spelling | doaj.art-6cfff7a5c9db431a9e7f2e7d8a5f647f2023-11-17T11:53:52ZengMDPI AGJournal of Functional Biomaterials2079-49832023-02-0114312010.3390/jfb14030120Collagen Membranes Functionalized with 150 Cycles of Atomic Layer Deposited Titania Improve Osteopromotive Property in Critical-Size Defects Created on Rat CalvariaLeonardo P. Faverani0Sarah Hashemi Astaneh1Monique Gonçalves da Costa2Leonardo A. Delanora3Tiburtino J. Lima-Neto4Stéfany Barbosa5Maretaningtias Dwi Ariani6Christos Takoudis7Cortino Sukotjo8Department of Diagnosis and Surgery, School of Dentistry, Sao Paulo State University (UNESP), Aracatuba 16015-050, BrazilDepartment of Chemical Engineering, University of Illinois Chicago, Chicago, IL 60612, USADepartment of Diagnosis and Surgery, School of Dentistry, Sao Paulo State University (UNESP), Aracatuba 16015-050, BrazilDepartment of Diagnosis and Surgery, School of Dentistry, Sao Paulo State University (UNESP), Aracatuba 16015-050, BrazilDepartment of Diagnosis and Surgery, School of Dentistry, Sao Paulo State University (UNESP), Aracatuba 16015-050, BrazilDepartment of Diagnosis and Surgery, School of Dentistry, Sao Paulo State University (UNESP), Aracatuba 16015-050, BrazilFaculty of Dental Medicine, Universitas Airlangga, Surabaya 60132, IndonesiaDepartment of Chemical Engineering, University of Illinois Chicago, Chicago, IL 60612, USAFaculty of Dental Medicine, Universitas Airlangga, Surabaya 60132, IndonesiaThe membranes used in bone reconstructions have been the object of investigation in the field of tissue engineering, seeking to improve their mechanical strength and add other properties, mainly the osteopromotive. This study aimed to evaluate the functionalization of collagen membranes, with atomic layer deposition of TiO<sub>2</sub> on the bone repair of critical defects in rat calvaria and subcutaneous biocompatibility. A total of 39 male rats were randomized into four groups: blood clot (BC), collagen membrane (COL), COL 150—150 cycles of titania, and COL 600—600 cycles of titania. The defects were created in each calvaria (5 mm in diameter) and covered according to each group; the animals were euthanized at 7, 14, and 28 days. The collected samples were assessed by histometric (newly bone formed, soft tissue area, membrane area, and residual linear defect) and histologic (inflammatory cells and blood cells count) analysis. All data were subjected to statistical analysis (<i>p</i> < 0.05). The COL150 group showed statistically significant differences compared to the other groups, mainly in the analysis of residual linear defects (1.5 ± 0.5 × 10<sup>6</sup> pixels/µm<sup>2</sup> for COL 150, and around 1 ± 0.5 × 10<sup>6</sup> pixels/µm<sup>2</sup> for the other groups) and newly formed bone (1500 ± 1200 pixels/µm for COL 150, and around 4000 pixels/µm for the others) (<i>p</i> < 0.05), demonstrating a better biological behavior in the chronology of defects repair. It is concluded that the collagen membrane functionalized by TiO<sub>2</sub> over 150 cycles showed better bioactive potential in treating critical size defects in the rats’ calvaria.https://www.mdpi.com/2079-4983/14/3/120atomic layer depositionmembrane functionalizationtitanium dioxideosseointegration |
| spellingShingle | Leonardo P. Faverani Sarah Hashemi Astaneh Monique Gonçalves da Costa Leonardo A. Delanora Tiburtino J. Lima-Neto Stéfany Barbosa Maretaningtias Dwi Ariani Christos Takoudis Cortino Sukotjo Collagen Membranes Functionalized with 150 Cycles of Atomic Layer Deposited Titania Improve Osteopromotive Property in Critical-Size Defects Created on Rat Calvaria Journal of Functional Biomaterials atomic layer deposition membrane functionalization titanium dioxide osseointegration |
| title | Collagen Membranes Functionalized with 150 Cycles of Atomic Layer Deposited Titania Improve Osteopromotive Property in Critical-Size Defects Created on Rat Calvaria |
| title_full | Collagen Membranes Functionalized with 150 Cycles of Atomic Layer Deposited Titania Improve Osteopromotive Property in Critical-Size Defects Created on Rat Calvaria |
| title_fullStr | Collagen Membranes Functionalized with 150 Cycles of Atomic Layer Deposited Titania Improve Osteopromotive Property in Critical-Size Defects Created on Rat Calvaria |
| title_full_unstemmed | Collagen Membranes Functionalized with 150 Cycles of Atomic Layer Deposited Titania Improve Osteopromotive Property in Critical-Size Defects Created on Rat Calvaria |
| title_short | Collagen Membranes Functionalized with 150 Cycles of Atomic Layer Deposited Titania Improve Osteopromotive Property in Critical-Size Defects Created on Rat Calvaria |
| title_sort | collagen membranes functionalized with 150 cycles of atomic layer deposited titania improve osteopromotive property in critical size defects created on rat calvaria |
| topic | atomic layer deposition membrane functionalization titanium dioxide osseointegration |
| url | https://www.mdpi.com/2079-4983/14/3/120 |
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