Appearance of choline metabolites in plasma and milk when choline is infused into the abomasum with or without methionine
Four lactating, ruminally cannulated Jersey cows, (mean ± standard deviation) 264 ± 54.2 d in milk and 484 ± 24.1 kg of body weight, were arranged in a 4 × 4 Latin square design to measure the effects of abomasal infusion of choline chloride with or without dl-Met on milk and plasma choline metaboli...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-11-01
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| Series: | JDS Communications |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666910223000790 |
| _version_ | 1827762370720563200 |
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| author | C.J.R. Jenkins P.J. Kononoff |
| author_facet | C.J.R. Jenkins P.J. Kononoff |
| author_sort | C.J.R. Jenkins |
| collection | DOAJ |
| description | Four lactating, ruminally cannulated Jersey cows, (mean ± standard deviation) 264 ± 54.2 d in milk and 484 ± 24.1 kg of body weight, were arranged in a 4 × 4 Latin square design to measure the effects of abomasal infusion of choline chloride with or without dl-Met on milk and plasma choline metabolites and plasma AA in cows fed a Met-deficient diet. Cows were randomly assigned to 1 of 4 experimental treatments: (1) control; no supplemental Met or choline (CON), (2) 13 g/d of choline ion delivered via abomasal infusion (CHO), (3) 13 g/d of Met delivered via abomasal infusion (MET), and (4) 13 g/d of choline and 13 g/d of Met delivered via abomasal infusion (CHO + MET). Cows received the same basal diet throughout the experiment, which was formulated to be deficient in Met (−5.0 g of Met using the NASEM, 2021, model). Periods were 7 d in length with d 1 to 2 serving as a wash-out period and cows being infused on d 3 to 7. Milk samples were collected twice daily on d 5 to 7 and were analyzed for fat, true protein, lactose, and choline metabolites including betaine, phosphocholine, and free choline using hydrophilic interaction liquid chromatography-tandem mass spectrometry. Blood samples were collected via venipuncture of the coccygeal vein at 1100, 1300, and 1500 h on d 7 of each period and were analyzed for free AA as well as choline metabolites. Plasma Met increased in response to Met infusion and an interaction with choline and Met infusion was observed in the plasma concentration of branched-chain AA. Cows receiving choline exhibited the greatest Cho yield in milk. Milk phosphocholine yield tended to be highest when both choline and Met were infused. |
| first_indexed | 2024-03-11T10:29:45Z |
| format | Article |
| id | doaj.art-6d03b6478c624ffaae82117914ade913 |
| institution | Directory Open Access Journal |
| issn | 2666-9102 |
| language | English |
| last_indexed | 2024-03-11T10:29:45Z |
| publishDate | 2023-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | JDS Communications |
| spelling | doaj.art-6d03b6478c624ffaae82117914ade9132023-11-15T04:12:13ZengElsevierJDS Communications2666-91022023-11-0146443448Appearance of choline metabolites in plasma and milk when choline is infused into the abomasum with or without methionineC.J.R. Jenkins0P.J. Kononoff1Department of Animal Science, University of Nebraska–Lincoln, Lincoln, NE 68583; Standard Dairy Consultants, Omaha, NE 68144Department of Animal Science, University of Nebraska–Lincoln, Lincoln, NE 68583; Corresponding authorFour lactating, ruminally cannulated Jersey cows, (mean ± standard deviation) 264 ± 54.2 d in milk and 484 ± 24.1 kg of body weight, were arranged in a 4 × 4 Latin square design to measure the effects of abomasal infusion of choline chloride with or without dl-Met on milk and plasma choline metabolites and plasma AA in cows fed a Met-deficient diet. Cows were randomly assigned to 1 of 4 experimental treatments: (1) control; no supplemental Met or choline (CON), (2) 13 g/d of choline ion delivered via abomasal infusion (CHO), (3) 13 g/d of Met delivered via abomasal infusion (MET), and (4) 13 g/d of choline and 13 g/d of Met delivered via abomasal infusion (CHO + MET). Cows received the same basal diet throughout the experiment, which was formulated to be deficient in Met (−5.0 g of Met using the NASEM, 2021, model). Periods were 7 d in length with d 1 to 2 serving as a wash-out period and cows being infused on d 3 to 7. Milk samples were collected twice daily on d 5 to 7 and were analyzed for fat, true protein, lactose, and choline metabolites including betaine, phosphocholine, and free choline using hydrophilic interaction liquid chromatography-tandem mass spectrometry. Blood samples were collected via venipuncture of the coccygeal vein at 1100, 1300, and 1500 h on d 7 of each period and were analyzed for free AA as well as choline metabolites. Plasma Met increased in response to Met infusion and an interaction with choline and Met infusion was observed in the plasma concentration of branched-chain AA. Cows receiving choline exhibited the greatest Cho yield in milk. Milk phosphocholine yield tended to be highest when both choline and Met were infused.http://www.sciencedirect.com/science/article/pii/S2666910223000790 |
| spellingShingle | C.J.R. Jenkins P.J. Kononoff Appearance of choline metabolites in plasma and milk when choline is infused into the abomasum with or without methionine JDS Communications |
| title | Appearance of choline metabolites in plasma and milk when choline is infused into the abomasum with or without methionine |
| title_full | Appearance of choline metabolites in plasma and milk when choline is infused into the abomasum with or without methionine |
| title_fullStr | Appearance of choline metabolites in plasma and milk when choline is infused into the abomasum with or without methionine |
| title_full_unstemmed | Appearance of choline metabolites in plasma and milk when choline is infused into the abomasum with or without methionine |
| title_short | Appearance of choline metabolites in plasma and milk when choline is infused into the abomasum with or without methionine |
| title_sort | appearance of choline metabolites in plasma and milk when choline is infused into the abomasum with or without methionine |
| url | http://www.sciencedirect.com/science/article/pii/S2666910223000790 |
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