[<sup>68</sup>Ga]Ga-PSMA and [<sup>68</sup>Ga]Ga-RM2 PET/MRI vs. Histopathological Images in Prostate Cancer: A New Workflow for Spatial Co-Registration
This study proposed a new workflow for co-registering prostate PET images from a dual-tracer PET/MRI study with histopathological images of resected prostate specimens. The method aims to establish an accurate correspondence between PET/MRI findings and histology, facilitating a deeper understanding...
| Main Authors: | , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-08-01
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| Series: | Bioengineering |
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| Online Access: | https://www.mdpi.com/2306-5354/10/8/953 |
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| author | Samuele Ghezzo Ilaria Neri Paola Mapelli Annarita Savi Ana Maria Samanes Gajate Giorgio Brembilla Carolina Bezzi Beatrice Maghini Tommaso Villa Alberto Briganti Francesco Montorsi Francesco De Cobelli Massimo Freschi Arturo Chiti Maria Picchio Paola Scifo |
| author_facet | Samuele Ghezzo Ilaria Neri Paola Mapelli Annarita Savi Ana Maria Samanes Gajate Giorgio Brembilla Carolina Bezzi Beatrice Maghini Tommaso Villa Alberto Briganti Francesco Montorsi Francesco De Cobelli Massimo Freschi Arturo Chiti Maria Picchio Paola Scifo |
| author_sort | Samuele Ghezzo |
| collection | DOAJ |
| description | This study proposed a new workflow for co-registering prostate PET images from a dual-tracer PET/MRI study with histopathological images of resected prostate specimens. The method aims to establish an accurate correspondence between PET/MRI findings and histology, facilitating a deeper understanding of PET tracer distribution and enabling advanced analyses like radiomics. To achieve this, images derived by three patients who underwent both [<sup>68</sup>Ga]Ga-PSMA and [<sup>68</sup>Ga]Ga-RM2 PET/MRI before radical prostatectomy were selected. After surgery, in the resected fresh specimens, fiducial markers visible on both histology and MR images were inserted. An ex vivo MRI of the prostate served as an intermediate step for co-registration between histological specimens and in vivo MRI examinations. The co-registration workflow involved five steps, ensuring alignment between histopathological images and PET/MRI data. The target registration error (TRE) was calculated to assess the precision of the co-registration. Furthermore, the DICE score was computed between the dominant intraprostatic tumor lesions delineated by the pathologist and the nuclear medicine physician. The TRE for the co-registration of histopathology and in vivo images was 1.59 mm, while the DICE score related to the site of increased intraprostatic uptake on [<sup>68</sup>Ga]Ga-PSMA and [<sup>68</sup>Ga]Ga-RM2 PET images was 0.54 and 0.75, respectively. This work shows an accurate co-registration method for histopathological and in vivo PET/MRI prostate examinations that allows the quantitative assessment of dual-tracer PET/MRI diagnostic accuracy at a millimetric scale. This approach may unveil radiotracer uptake mechanisms and identify new PET/MRI biomarkers, thus establishing the basis for precision medicine and future analyses, such as radiomics. |
| first_indexed | 2024-03-11T00:06:31Z |
| format | Article |
| id | doaj.art-6d08ba3933544426a56629fcb8d47a92 |
| institution | Directory Open Access Journal |
| issn | 2306-5354 |
| language | English |
| last_indexed | 2024-03-11T00:06:31Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Bioengineering |
| spelling | doaj.art-6d08ba3933544426a56629fcb8d47a922023-11-19T00:18:18ZengMDPI AGBioengineering2306-53542023-08-0110895310.3390/bioengineering10080953[<sup>68</sup>Ga]Ga-PSMA and [<sup>68</sup>Ga]Ga-RM2 PET/MRI vs. Histopathological Images in Prostate Cancer: A New Workflow for Spatial Co-RegistrationSamuele Ghezzo0Ilaria Neri1Paola Mapelli2Annarita Savi3Ana Maria Samanes Gajate4Giorgio Brembilla5Carolina Bezzi6Beatrice Maghini7Tommaso Villa8Alberto Briganti9Francesco Montorsi10Francesco De Cobelli11Massimo Freschi12Arturo Chiti13Maria Picchio14Paola Scifo15Faculty of Medicine and Surgery, Vita-Salute San Raffaele University, Via Olgettina 58, 20132 Milan, ItalyFaculty of Medicine and Surgery, Vita-Salute San Raffaele University, Via Olgettina 58, 20132 Milan, ItalyFaculty of Medicine and Surgery, Vita-Salute San Raffaele University, Via Olgettina 58, 20132 Milan, ItalyDepartment of Nuclear Medicine, IRCCS San Raffaele Scientific Institute, Via Olgettina 60, 20132 Milan, ItalyDepartment of Nuclear Medicine, IRCCS San Raffaele Scientific Institute, Via Olgettina 60, 20132 Milan, ItalyFaculty of Medicine and Surgery, Vita-Salute San Raffaele University, Via Olgettina 58, 20132 Milan, ItalyFaculty of Medicine and Surgery, Vita-Salute San Raffaele University, Via Olgettina 58, 20132 Milan, ItalyDepartment of Pathology, IRCCS San Raffaele Scientific Institute, Via Olgettina 60, 20132 Milan, ItalyFaculty of Medicine and Surgery, Vita-Salute San Raffaele University, Via Olgettina 58, 20132 Milan, ItalyFaculty of Medicine and Surgery, Vita-Salute San Raffaele University, Via Olgettina 58, 20132 Milan, ItalyFaculty of Medicine and Surgery, Vita-Salute San Raffaele University, Via Olgettina 58, 20132 Milan, ItalyFaculty of Medicine and Surgery, Vita-Salute San Raffaele University, Via Olgettina 58, 20132 Milan, ItalyDepartment of Pathology, IRCCS San Raffaele Scientific Institute, Via Olgettina 60, 20132 Milan, ItalyFaculty of Medicine and Surgery, Vita-Salute San Raffaele University, Via Olgettina 58, 20132 Milan, ItalyFaculty of Medicine and Surgery, Vita-Salute San Raffaele University, Via Olgettina 58, 20132 Milan, ItalyDepartment of Nuclear Medicine, IRCCS San Raffaele Scientific Institute, Via Olgettina 60, 20132 Milan, ItalyThis study proposed a new workflow for co-registering prostate PET images from a dual-tracer PET/MRI study with histopathological images of resected prostate specimens. The method aims to establish an accurate correspondence between PET/MRI findings and histology, facilitating a deeper understanding of PET tracer distribution and enabling advanced analyses like radiomics. To achieve this, images derived by three patients who underwent both [<sup>68</sup>Ga]Ga-PSMA and [<sup>68</sup>Ga]Ga-RM2 PET/MRI before radical prostatectomy were selected. After surgery, in the resected fresh specimens, fiducial markers visible on both histology and MR images were inserted. An ex vivo MRI of the prostate served as an intermediate step for co-registration between histological specimens and in vivo MRI examinations. The co-registration workflow involved five steps, ensuring alignment between histopathological images and PET/MRI data. The target registration error (TRE) was calculated to assess the precision of the co-registration. Furthermore, the DICE score was computed between the dominant intraprostatic tumor lesions delineated by the pathologist and the nuclear medicine physician. The TRE for the co-registration of histopathology and in vivo images was 1.59 mm, while the DICE score related to the site of increased intraprostatic uptake on [<sup>68</sup>Ga]Ga-PSMA and [<sup>68</sup>Ga]Ga-RM2 PET images was 0.54 and 0.75, respectively. This work shows an accurate co-registration method for histopathological and in vivo PET/MRI prostate examinations that allows the quantitative assessment of dual-tracer PET/MRI diagnostic accuracy at a millimetric scale. This approach may unveil radiotracer uptake mechanisms and identify new PET/MRI biomarkers, thus establishing the basis for precision medicine and future analyses, such as radiomics.https://www.mdpi.com/2306-5354/10/8/953PET/MRIhistopathologyco-registrationprostate |
| spellingShingle | Samuele Ghezzo Ilaria Neri Paola Mapelli Annarita Savi Ana Maria Samanes Gajate Giorgio Brembilla Carolina Bezzi Beatrice Maghini Tommaso Villa Alberto Briganti Francesco Montorsi Francesco De Cobelli Massimo Freschi Arturo Chiti Maria Picchio Paola Scifo [<sup>68</sup>Ga]Ga-PSMA and [<sup>68</sup>Ga]Ga-RM2 PET/MRI vs. Histopathological Images in Prostate Cancer: A New Workflow for Spatial Co-Registration Bioengineering PET/MRI histopathology co-registration prostate |
| title | [<sup>68</sup>Ga]Ga-PSMA and [<sup>68</sup>Ga]Ga-RM2 PET/MRI vs. Histopathological Images in Prostate Cancer: A New Workflow for Spatial Co-Registration |
| title_full | [<sup>68</sup>Ga]Ga-PSMA and [<sup>68</sup>Ga]Ga-RM2 PET/MRI vs. Histopathological Images in Prostate Cancer: A New Workflow for Spatial Co-Registration |
| title_fullStr | [<sup>68</sup>Ga]Ga-PSMA and [<sup>68</sup>Ga]Ga-RM2 PET/MRI vs. Histopathological Images in Prostate Cancer: A New Workflow for Spatial Co-Registration |
| title_full_unstemmed | [<sup>68</sup>Ga]Ga-PSMA and [<sup>68</sup>Ga]Ga-RM2 PET/MRI vs. Histopathological Images in Prostate Cancer: A New Workflow for Spatial Co-Registration |
| title_short | [<sup>68</sup>Ga]Ga-PSMA and [<sup>68</sup>Ga]Ga-RM2 PET/MRI vs. Histopathological Images in Prostate Cancer: A New Workflow for Spatial Co-Registration |
| title_sort | sup 68 sup ga ga psma and sup 68 sup ga ga rm2 pet mri vs histopathological images in prostate cancer a new workflow for spatial co registration |
| topic | PET/MRI histopathology co-registration prostate |
| url | https://www.mdpi.com/2306-5354/10/8/953 |
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