Functional Characterization of a Sugar Beet <i>BvbHLH93</i> Transcription Factor in Salt Stress Tolerance
The basic/helix–loop–helix (bHLH) transcription factor (TF) plays an important role for plant growth, development, and stress responses. Previously, proteomics of NaCl treated sugar beet leaves revealed that a bHLH TF, <i>BvbHLH93</i>, was significantly increased under salt stress. The B...
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MDPI AG
2021-04-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/7/3669 |
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| author | Yuguang Wang Shuang Wang Ye Tian Qiuhong Wang Sixue Chen Hongli Li Chunquan Ma Haiying Li |
| author_facet | Yuguang Wang Shuang Wang Ye Tian Qiuhong Wang Sixue Chen Hongli Li Chunquan Ma Haiying Li |
| author_sort | Yuguang Wang |
| collection | DOAJ |
| description | The basic/helix–loop–helix (bHLH) transcription factor (TF) plays an important role for plant growth, development, and stress responses. Previously, proteomics of NaCl treated sugar beet leaves revealed that a bHLH TF, <i>BvbHLH93</i>, was significantly increased under salt stress. The BvbHLH93 protein localized in the nucleus and exhibited activation activity. The expression of <i>BvbHLH93</i> was significantly up-regulated in roots and leaves by salt stress, and the highest expression level in roots and leaves was 24 and 48 h after salt stress, respectively. Furthermore, constitutive expression of <i>BvbHLH93</i> conferred enhanced salt tolerance in <i>Arabidopsis,</i> as indicated by longer roots and higher content of chlorophyll than wild type. Additionally, the ectopic expression lines accumulated less Na<sup>+</sup> and MDA, but more K<sup>+</sup> than the WT. Overexpression of the <i>BvBHLH93</i> enhanced the activities of antioxidant enzymes by positively regulating the expression of antioxidant genes <i>SOD</i> and <i>POD</i>. Compared to WT, the overexpression plants also had low expression levels of <i>RbohD</i> and <i>RbohF</i>, which are involved in reactive oxygen species (ROS) production. These results suggest that <i>BvbHLH93</i> plays a key role in enhancing salt stress tolerance by enhancing antioxidant enzymes and decreasing ROS generation. |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T12:42:36Z |
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| spelling | doaj.art-6d0bcfe01283493d9c045e7a3e84cb402023-11-21T13:47:14ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-04-01227366910.3390/ijms22073669Functional Characterization of a Sugar Beet <i>BvbHLH93</i> Transcription Factor in Salt Stress ToleranceYuguang Wang0Shuang Wang1Ye Tian2Qiuhong Wang3Sixue Chen4Hongli Li5Chunquan Ma6Haiying Li7Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education, Heilongjiang University, Harbin 150080, ChinaEngineering Research Center of Agricultural Microbiology Technology, Ministry of Education, Heilongjiang University, Harbin 150080, ChinaEngineering Research Center of Agricultural Microbiology Technology, Ministry of Education, Heilongjiang University, Harbin 150080, ChinaNational Sugar Crop Improvement Centre, College of Advanced Agriculture and Ecological Environment, Heilongjiang University, Harbin 150080, ChinaPlant Molecular and Cellular Biology Program, Department of Biology, Genetics Institute, University of Florida, Gainesville, FL 32610, USAEngineering Research Center of Agricultural Microbiology Technology, Ministry of Education, Heilongjiang University, Harbin 150080, ChinaEngineering Research Center of Agricultural Microbiology Technology, Ministry of Education, Heilongjiang University, Harbin 150080, ChinaEngineering Research Center of Agricultural Microbiology Technology, Ministry of Education, Heilongjiang University, Harbin 150080, ChinaThe basic/helix–loop–helix (bHLH) transcription factor (TF) plays an important role for plant growth, development, and stress responses. Previously, proteomics of NaCl treated sugar beet leaves revealed that a bHLH TF, <i>BvbHLH93</i>, was significantly increased under salt stress. The BvbHLH93 protein localized in the nucleus and exhibited activation activity. The expression of <i>BvbHLH93</i> was significantly up-regulated in roots and leaves by salt stress, and the highest expression level in roots and leaves was 24 and 48 h after salt stress, respectively. Furthermore, constitutive expression of <i>BvbHLH93</i> conferred enhanced salt tolerance in <i>Arabidopsis,</i> as indicated by longer roots and higher content of chlorophyll than wild type. Additionally, the ectopic expression lines accumulated less Na<sup>+</sup> and MDA, but more K<sup>+</sup> than the WT. Overexpression of the <i>BvBHLH93</i> enhanced the activities of antioxidant enzymes by positively regulating the expression of antioxidant genes <i>SOD</i> and <i>POD</i>. Compared to WT, the overexpression plants also had low expression levels of <i>RbohD</i> and <i>RbohF</i>, which are involved in reactive oxygen species (ROS) production. These results suggest that <i>BvbHLH93</i> plays a key role in enhancing salt stress tolerance by enhancing antioxidant enzymes and decreasing ROS generation.https://www.mdpi.com/1422-0067/22/7/3669sugar beetsalt stressbHLHtranscription factorantioxidant enzymes |
| spellingShingle | Yuguang Wang Shuang Wang Ye Tian Qiuhong Wang Sixue Chen Hongli Li Chunquan Ma Haiying Li Functional Characterization of a Sugar Beet <i>BvbHLH93</i> Transcription Factor in Salt Stress Tolerance International Journal of Molecular Sciences sugar beet salt stress bHLH transcription factor antioxidant enzymes |
| title | Functional Characterization of a Sugar Beet <i>BvbHLH93</i> Transcription Factor in Salt Stress Tolerance |
| title_full | Functional Characterization of a Sugar Beet <i>BvbHLH93</i> Transcription Factor in Salt Stress Tolerance |
| title_fullStr | Functional Characterization of a Sugar Beet <i>BvbHLH93</i> Transcription Factor in Salt Stress Tolerance |
| title_full_unstemmed | Functional Characterization of a Sugar Beet <i>BvbHLH93</i> Transcription Factor in Salt Stress Tolerance |
| title_short | Functional Characterization of a Sugar Beet <i>BvbHLH93</i> Transcription Factor in Salt Stress Tolerance |
| title_sort | functional characterization of a sugar beet i bvbhlh93 i transcription factor in salt stress tolerance |
| topic | sugar beet salt stress bHLH transcription factor antioxidant enzymes |
| url | https://www.mdpi.com/1422-0067/22/7/3669 |
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