A Universal Fluorescent Immunochromatography Assay Based on Quantum Dot Nanoparticles for the Rapid Detection of Specific Antibodies against SARS-CoV-2 Nucleocapsid Protein
Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is the pathogenic agent leading to COVID-19. Due to high speed of transmission and mutation rates, universal diagnosis and appropriate prevention are still urgently needed. The nucleocapsid protein of SARS-CoV-2 is considered more conserve...
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| Format: | Article |
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MDPI AG
2022-06-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/11/6225 |
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| author | Zehui Li Aiping Wang Jingming Zhou Yumei Chen Hongliang Liu Yankai Liu Ying Zhang Peiyang Ding Xifang Zhu Chao Liang Yanhua Qi Enping Liu Gaiping Zhang |
| author_facet | Zehui Li Aiping Wang Jingming Zhou Yumei Chen Hongliang Liu Yankai Liu Ying Zhang Peiyang Ding Xifang Zhu Chao Liang Yanhua Qi Enping Liu Gaiping Zhang |
| author_sort | Zehui Li |
| collection | DOAJ |
| description | Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is the pathogenic agent leading to COVID-19. Due to high speed of transmission and mutation rates, universal diagnosis and appropriate prevention are still urgently needed. The nucleocapsid protein of SARS-CoV-2 is considered more conserved than spike proteins and is abundant during the virus’ life cycle, making it suitable for diagnostic applications. Here, we designed and developed a fluorescent immunochromatography assay (FICA) for the rapid detection of SARS-CoV-2-specific antibodies using ZnCdSe/ZnS QDs-conjugated nucleocapsid (N) proteins as probes. The nucleocapsid protein was expressed in <i>E.coli</i> and purified via Ni-NTA affinity chromatography with considerable concentration (0.762 mg/mL) and a purity of more than 90%, which could bind to specific antibodies and the complex could be captured by <i>Staphylococcal</i> protein A (SPA) with fluorescence displayed. After the optimization of coupling and detecting conditions, the limit of detection was determined to be 1:1.024 × 10<sup>5</sup> with an IgG concentration of 48.84 ng/mL with good specificity shown to antibodies against other zoonotic coronaviruses and respiratory infection-related viruses (<i>n</i> = 5). The universal fluorescent immunochromatography assay simplified operation processes in one step, which could be used for the point of care detection of SARS-CoV-2-specific antibodies. Moreover, it was also considered as an efficient tool for the serological screening of potential susceptible animals and for monitoring the expansion of virus host ranges. |
| first_indexed | 2024-03-10T01:14:10Z |
| format | Article |
| id | doaj.art-6d14a7543be74a3ba5d23296d48d7d10 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T01:14:10Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-6d14a7543be74a3ba5d23296d48d7d102023-11-23T14:11:51ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-06-012311622510.3390/ijms23116225A Universal Fluorescent Immunochromatography Assay Based on Quantum Dot Nanoparticles for the Rapid Detection of Specific Antibodies against SARS-CoV-2 Nucleocapsid ProteinZehui Li0Aiping Wang1Jingming Zhou2Yumei Chen3Hongliang Liu4Yankai Liu5Ying Zhang6Peiyang Ding7Xifang Zhu8Chao Liang9Yanhua Qi10Enping Liu11Gaiping Zhang12School of Life Science, Zhengzhou University, Zhengzhou 450001, ChinaSchool of Life Science, Zhengzhou University, Zhengzhou 450001, ChinaSchool of Life Science, Zhengzhou University, Zhengzhou 450001, ChinaSchool of Life Science, Zhengzhou University, Zhengzhou 450001, ChinaSchool of Life Science, Zhengzhou University, Zhengzhou 450001, ChinaSchool of Life Science, Zhengzhou University, Zhengzhou 450001, ChinaSchool of Life Science, Zhengzhou University, Zhengzhou 450001, ChinaSchool of Life Science, Zhengzhou University, Zhengzhou 450001, ChinaSchool of Life Science, Zhengzhou University, Zhengzhou 450001, ChinaSchool of Life Science, Zhengzhou University, Zhengzhou 450001, ChinaSchool of Life Science, Zhengzhou University, Zhengzhou 450001, ChinaSchool of Life Science, Zhengzhou University, Zhengzhou 450001, ChinaSchool of Life Science, Zhengzhou University, Zhengzhou 450001, ChinaSevere Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is the pathogenic agent leading to COVID-19. Due to high speed of transmission and mutation rates, universal diagnosis and appropriate prevention are still urgently needed. The nucleocapsid protein of SARS-CoV-2 is considered more conserved than spike proteins and is abundant during the virus’ life cycle, making it suitable for diagnostic applications. Here, we designed and developed a fluorescent immunochromatography assay (FICA) for the rapid detection of SARS-CoV-2-specific antibodies using ZnCdSe/ZnS QDs-conjugated nucleocapsid (N) proteins as probes. The nucleocapsid protein was expressed in <i>E.coli</i> and purified via Ni-NTA affinity chromatography with considerable concentration (0.762 mg/mL) and a purity of more than 90%, which could bind to specific antibodies and the complex could be captured by <i>Staphylococcal</i> protein A (SPA) with fluorescence displayed. After the optimization of coupling and detecting conditions, the limit of detection was determined to be 1:1.024 × 10<sup>5</sup> with an IgG concentration of 48.84 ng/mL with good specificity shown to antibodies against other zoonotic coronaviruses and respiratory infection-related viruses (<i>n</i> = 5). The universal fluorescent immunochromatography assay simplified operation processes in one step, which could be used for the point of care detection of SARS-CoV-2-specific antibodies. Moreover, it was also considered as an efficient tool for the serological screening of potential susceptible animals and for monitoring the expansion of virus host ranges.https://www.mdpi.com/1422-0067/23/11/6225SARS-CoV-2fluorescent immunochromatography assaynucleocapsid proteinquantum dotspoint-of-care detection |
| spellingShingle | Zehui Li Aiping Wang Jingming Zhou Yumei Chen Hongliang Liu Yankai Liu Ying Zhang Peiyang Ding Xifang Zhu Chao Liang Yanhua Qi Enping Liu Gaiping Zhang A Universal Fluorescent Immunochromatography Assay Based on Quantum Dot Nanoparticles for the Rapid Detection of Specific Antibodies against SARS-CoV-2 Nucleocapsid Protein International Journal of Molecular Sciences SARS-CoV-2 fluorescent immunochromatography assay nucleocapsid protein quantum dots point-of-care detection |
| title | A Universal Fluorescent Immunochromatography Assay Based on Quantum Dot Nanoparticles for the Rapid Detection of Specific Antibodies against SARS-CoV-2 Nucleocapsid Protein |
| title_full | A Universal Fluorescent Immunochromatography Assay Based on Quantum Dot Nanoparticles for the Rapid Detection of Specific Antibodies against SARS-CoV-2 Nucleocapsid Protein |
| title_fullStr | A Universal Fluorescent Immunochromatography Assay Based on Quantum Dot Nanoparticles for the Rapid Detection of Specific Antibodies against SARS-CoV-2 Nucleocapsid Protein |
| title_full_unstemmed | A Universal Fluorescent Immunochromatography Assay Based on Quantum Dot Nanoparticles for the Rapid Detection of Specific Antibodies against SARS-CoV-2 Nucleocapsid Protein |
| title_short | A Universal Fluorescent Immunochromatography Assay Based on Quantum Dot Nanoparticles for the Rapid Detection of Specific Antibodies against SARS-CoV-2 Nucleocapsid Protein |
| title_sort | universal fluorescent immunochromatography assay based on quantum dot nanoparticles for the rapid detection of specific antibodies against sars cov 2 nucleocapsid protein |
| topic | SARS-CoV-2 fluorescent immunochromatography assay nucleocapsid protein quantum dots point-of-care detection |
| url | https://www.mdpi.com/1422-0067/23/11/6225 |
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