CrMPK3, a mitogen activated protein kinase from <it>Catharanthus roseus</it> and its possible role in stress induced biosynthesis of monoterpenoid indole alkaloids
<p>Abstract</p> <p>Background</p> <p>Mitogen activated protein kinase (MAPK) cascade is an important signaling cascade that operates in stress signal transduction in plants. The biologically active monoterpenoid indole alkaloids (MIA) produced in <it>Catharanthus...
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| Format: | Article |
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BMC
2012-08-01
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| Series: | BMC Plant Biology |
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| Online Access: | http://www.biomedcentral.com/1471-2229/12/134 |
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| author | Raina Susheel Wankhede Dhammaprakash Jaggi Monika Singh Pallavi Jalmi Siddhi Raghuram Badmi Sheikh Arsheed Sinha Alok |
| author_facet | Raina Susheel Wankhede Dhammaprakash Jaggi Monika Singh Pallavi Jalmi Siddhi Raghuram Badmi Sheikh Arsheed Sinha Alok |
| author_sort | Raina Susheel |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Mitogen activated protein kinase (MAPK) cascade is an important signaling cascade that operates in stress signal transduction in plants. The biologically active monoterpenoid indole alkaloids (MIA) produced in <it>Catharanthus roseus</it> are known to be induced under several abiotic stress conditions such as wounding, UV-B etc. However involvement of any signaling component in the accumulation of MIAs remains poorly investigated so far. Here we report isolation of a novel abiotic stress inducible <it>Catharanthus roseus</it> MAPK, <it>CrMPK3</it> that may have role in accumulation of MIAs in response to abiotic stress.</p> <p>Results</p> <p>CrMPK3 expressed in bacterial system is an active kinase as it showed auto-phosphorylation and phosphorylation of Myelin Basic Protein. CrMPK3 though localized in cytoplasm, moves to nucleus upon wounding. Wounding, UV treatment and MeJA application on <it>C. roseus</it> leaves resulted in the transcript accumulation of <it>CrMPK3</it> as well as activation of MAPK in <it>C. roseus</it> leaves. Immuno-precipitation followed by immunoblot analysis revealed that wounding, UV treatment and methyl jasmonate (MeJA) activate CrMPK3<it>.</it> Transient over-expression of <it>CrMPK3</it> in <it>C. roseus</it> leaf tissue showed enhanced expression of key MIA biosynthesis pathway genes and also accumulation of specific MIAs.</p> <p>Conclusion</p> <p>Results from our study suggest a possible involvement of <it>CrMPK3</it> in abiotic stress signal transduction towards regulation of transcripts of key MIA biosynthetic pathway genes, regulators and accumulation of major MIAs.</p> |
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| format | Article |
| id | doaj.art-6d16235446aa4b19b01d5e70119e87cd |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-04-12T16:43:20Z |
| publishDate | 2012-08-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-6d16235446aa4b19b01d5e70119e87cd2022-12-22T03:24:41ZengBMCBMC Plant Biology1471-22292012-08-0112113410.1186/1471-2229-12-134CrMPK3, a mitogen activated protein kinase from <it>Catharanthus roseus</it> and its possible role in stress induced biosynthesis of monoterpenoid indole alkaloidsRaina SusheelWankhede DhammaprakashJaggi MonikaSingh PallaviJalmi SiddhiRaghuram BadmiSheikh ArsheedSinha Alok<p>Abstract</p> <p>Background</p> <p>Mitogen activated protein kinase (MAPK) cascade is an important signaling cascade that operates in stress signal transduction in plants. The biologically active monoterpenoid indole alkaloids (MIA) produced in <it>Catharanthus roseus</it> are known to be induced under several abiotic stress conditions such as wounding, UV-B etc. However involvement of any signaling component in the accumulation of MIAs remains poorly investigated so far. Here we report isolation of a novel abiotic stress inducible <it>Catharanthus roseus</it> MAPK, <it>CrMPK3</it> that may have role in accumulation of MIAs in response to abiotic stress.</p> <p>Results</p> <p>CrMPK3 expressed in bacterial system is an active kinase as it showed auto-phosphorylation and phosphorylation of Myelin Basic Protein. CrMPK3 though localized in cytoplasm, moves to nucleus upon wounding. Wounding, UV treatment and MeJA application on <it>C. roseus</it> leaves resulted in the transcript accumulation of <it>CrMPK3</it> as well as activation of MAPK in <it>C. roseus</it> leaves. Immuno-precipitation followed by immunoblot analysis revealed that wounding, UV treatment and methyl jasmonate (MeJA) activate CrMPK3<it>.</it> Transient over-expression of <it>CrMPK3</it> in <it>C. roseus</it> leaf tissue showed enhanced expression of key MIA biosynthesis pathway genes and also accumulation of specific MIAs.</p> <p>Conclusion</p> <p>Results from our study suggest a possible involvement of <it>CrMPK3</it> in abiotic stress signal transduction towards regulation of transcripts of key MIA biosynthetic pathway genes, regulators and accumulation of major MIAs.</p>http://www.biomedcentral.com/1471-2229/12/134<it>Catharanthus roseus</it>Methyl jasmonateMitogen activated protein kinaseMonoterpenoid indole alkaloidSecondary metabolism |
| spellingShingle | Raina Susheel Wankhede Dhammaprakash Jaggi Monika Singh Pallavi Jalmi Siddhi Raghuram Badmi Sheikh Arsheed Sinha Alok CrMPK3, a mitogen activated protein kinase from <it>Catharanthus roseus</it> and its possible role in stress induced biosynthesis of monoterpenoid indole alkaloids BMC Plant Biology <it>Catharanthus roseus</it> Methyl jasmonate Mitogen activated protein kinase Monoterpenoid indole alkaloid Secondary metabolism |
| title | CrMPK3, a mitogen activated protein kinase from <it>Catharanthus roseus</it> and its possible role in stress induced biosynthesis of monoterpenoid indole alkaloids |
| title_full | CrMPK3, a mitogen activated protein kinase from <it>Catharanthus roseus</it> and its possible role in stress induced biosynthesis of monoterpenoid indole alkaloids |
| title_fullStr | CrMPK3, a mitogen activated protein kinase from <it>Catharanthus roseus</it> and its possible role in stress induced biosynthesis of monoterpenoid indole alkaloids |
| title_full_unstemmed | CrMPK3, a mitogen activated protein kinase from <it>Catharanthus roseus</it> and its possible role in stress induced biosynthesis of monoterpenoid indole alkaloids |
| title_short | CrMPK3, a mitogen activated protein kinase from <it>Catharanthus roseus</it> and its possible role in stress induced biosynthesis of monoterpenoid indole alkaloids |
| title_sort | crmpk3 a mitogen activated protein kinase from it catharanthus roseus it and its possible role in stress induced biosynthesis of monoterpenoid indole alkaloids |
| topic | <it>Catharanthus roseus</it> Methyl jasmonate Mitogen activated protein kinase Monoterpenoid indole alkaloid Secondary metabolism |
| url | http://www.biomedcentral.com/1471-2229/12/134 |
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