The Preparation of Liposomes derived from Mixed Micelles of Lecithin added by Sodium Cholate, followed by Dialysing using Hemoflow High Flux F60S
Liposomes are used for drug carriers meaning that drugs are incorporated in the membrane or the vesicle of the liposomes. In this study, liposomes were prepared from mixed micelles, consisting of phosphatidylcholine, without or with cholesterol and sodium cholate was added in several ratios namely 0...
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| Format: | Article |
| Language: | English |
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Universitas Indonesia
2010-10-01
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| Series: | Makara Journal of Health Research |
| Subjects: | |
| Online Access: | http://journal.ui.ac.id/index.php/health/article/view/305 |
| _version_ | 1827844782851883008 |
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| author | Erni Hernawati Purwaningsih Rolf Schubert |
| author_facet | Erni Hernawati Purwaningsih Rolf Schubert |
| author_sort | Erni Hernawati Purwaningsih |
| collection | DOAJ |
| description | Liposomes are used for drug carriers meaning that drugs are incorporated in the membrane or the vesicle of the liposomes. In this study, liposomes were prepared from mixed micelles, consisting of phosphatidylcholine, without or with cholesterol and sodium cholate was added in several ratios namely 0.44; 0.55; 0.63; 0.70; 0.90 and 1.10. After the preparation, the sodium cholate has been removed by a dialysis membrane, using the Hemoflow High Flux, which is generally used for haemodialysis. The Hemoflow High Flux is a tool in an effort to obtain a simple, quick, effective method for removing sodium cholate in the process of preparing liposomes. The effectiveness of this tool was proved by the particle size of the liposome which was measured by the Malvern Particle Sizer. The particle size of the liposome consisting of phosphatidylcholine (PC) without cholesterol and with cholesterol was 63-68 nm at all ratios and approximately 125 nm at the ratio of 0.55; 0.63; 0.70, respectively. The particle size of the liposome tended to be smaller after dialyzing, although the concentration of lipids tended to increase. However, a large amount of buffer solution has to be used with this method. |
| first_indexed | 2024-03-12T08:46:51Z |
| format | Article |
| id | doaj.art-6d185a69dfd7443597a957931a829d40 |
| institution | Directory Open Access Journal |
| issn | 2356-3664 2356-3656 |
| language | English |
| last_indexed | 2024-03-12T08:46:51Z |
| publishDate | 2010-10-01 |
| publisher | Universitas Indonesia |
| record_format | Article |
| series | Makara Journal of Health Research |
| spelling | doaj.art-6d185a69dfd7443597a957931a829d402023-09-02T16:25:42ZengUniversitas IndonesiaMakara Journal of Health Research2356-36642356-36562010-10-0182495210.7454/msk.v8i2.305297The Preparation of Liposomes derived from Mixed Micelles of Lecithin added by Sodium Cholate, followed by Dialysing using Hemoflow High Flux F60SErni Hernawati Purwaningsih0Rolf Schubert1Department of Medical Pharmacy, Faculty of Medicine, University of Indonesia, Jakarta 10430Institute of Pharmaceutical Technology, University of Freiburg, D-79104 FreiburgLiposomes are used for drug carriers meaning that drugs are incorporated in the membrane or the vesicle of the liposomes. In this study, liposomes were prepared from mixed micelles, consisting of phosphatidylcholine, without or with cholesterol and sodium cholate was added in several ratios namely 0.44; 0.55; 0.63; 0.70; 0.90 and 1.10. After the preparation, the sodium cholate has been removed by a dialysis membrane, using the Hemoflow High Flux, which is generally used for haemodialysis. The Hemoflow High Flux is a tool in an effort to obtain a simple, quick, effective method for removing sodium cholate in the process of preparing liposomes. The effectiveness of this tool was proved by the particle size of the liposome which was measured by the Malvern Particle Sizer. The particle size of the liposome consisting of phosphatidylcholine (PC) without cholesterol and with cholesterol was 63-68 nm at all ratios and approximately 125 nm at the ratio of 0.55; 0.63; 0.70, respectively. The particle size of the liposome tended to be smaller after dialyzing, although the concentration of lipids tended to increase. However, a large amount of buffer solution has to be used with this method.http://journal.ui.ac.id/index.php/health/article/view/305liposomemixed-micellesdialyzed membrane |
| spellingShingle | Erni Hernawati Purwaningsih Rolf Schubert The Preparation of Liposomes derived from Mixed Micelles of Lecithin added by Sodium Cholate, followed by Dialysing using Hemoflow High Flux F60S Makara Journal of Health Research liposome mixed-micelles dialyzed membrane |
| title | The Preparation of Liposomes derived from Mixed Micelles of Lecithin added by Sodium Cholate, followed by Dialysing using Hemoflow High Flux F60S |
| title_full | The Preparation of Liposomes derived from Mixed Micelles of Lecithin added by Sodium Cholate, followed by Dialysing using Hemoflow High Flux F60S |
| title_fullStr | The Preparation of Liposomes derived from Mixed Micelles of Lecithin added by Sodium Cholate, followed by Dialysing using Hemoflow High Flux F60S |
| title_full_unstemmed | The Preparation of Liposomes derived from Mixed Micelles of Lecithin added by Sodium Cholate, followed by Dialysing using Hemoflow High Flux F60S |
| title_short | The Preparation of Liposomes derived from Mixed Micelles of Lecithin added by Sodium Cholate, followed by Dialysing using Hemoflow High Flux F60S |
| title_sort | preparation of liposomes derived from mixed micelles of lecithin added by sodium cholate followed by dialysing using hemoflow high flux f60s |
| topic | liposome mixed-micelles dialyzed membrane |
| url | http://journal.ui.ac.id/index.php/health/article/view/305 |
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