RETRACTED ARTICLE: Syndecan-1 suppresses cell growth and migration via blocking JAK1/STAT3 and Ras/Raf/MEK/ERK pathways in human colorectal carcinoma cells
Abstract Background Syndecan-1 (SDC-1) is a crucial membrane proteoglycan, which is confirmed to participate in several tumor cell biological processes. However, the biological significance of SDC-1 in colorectal carcinoma is not yet clear. An objective of this study was to investigate the role of S...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2019-11-01
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| Series: | BMC Cancer |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12885-019-6381-y |
| _version_ | 1797274304353665024 |
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| author | Shaojun Wang Xiaofei Zhang Guimei Wang Bin Cao Hong Yang Lipeng Jin Mingjuan Cui Yongjun Mao |
| author_facet | Shaojun Wang Xiaofei Zhang Guimei Wang Bin Cao Hong Yang Lipeng Jin Mingjuan Cui Yongjun Mao |
| author_sort | Shaojun Wang |
| collection | DOAJ |
| description | Abstract Background Syndecan-1 (SDC-1) is a crucial membrane proteoglycan, which is confirmed to participate in several tumor cell biological processes. However, the biological significance of SDC-1 in colorectal carcinoma is not yet clear. An objective of this study was to investigate the role of SDC-1 in colorectal carcinoma cells. Methods Expression of SDC-1 in colorectal carcinoma tissues was evaluated by Reverse transcription-quantitative real-time PCR (RT-qPCR) and western blot. After transfection with pcDNA3.1 or pc-SDC-1, the transfection efficiency was measured. Next, SW480, SW620 and LOVO cell viability, apoptosis, migration and adhesion were assessed to explore the effects of exogenous overexpressed SDC-1 on colorectal carcinoma. In addition, the influences of aberrant expressed SDC-1 in Janus kinase 1 (JAK1)/signal transducer and activator of transcription 3 (STAT3) and rat sarcoma virus (Ras)/rapidly accelerated fibrosarcoma (Raf)/mitogen-activated protein kinase (MEK)/extracellular signal-regulated kinase (ERK) pathways were detected by western blot analysis. Results SDC-1 mRNA and protein levels were down-regulated in human colorectal carcinoma tissues. SDC-1 overexpression inhibited cell proliferation via suppressing CyclinD1 and c-Myc expression, meanwhile stimulated cell apoptosis via increasing the expression levels of B-cell lymphoma-2-associated x (Bax) and Cleaved-Caspase-3. Additionally, SDC-1 overexpression restrained cell migration via inhibiting the protein expression of matrix metallopeptidase 9 (MMP-9), and elicited cell adhesion through increasing intercellular cell adhesion molecule-1 (ICAM-1). Furthermore, SDC-1 overexpression suppressed JAK1/STAT3 and Ras/Raf/MEK/ERK-related protein levels. Conclusions In general, the evidence from this study suggested that SDC-1 suppressed cell growth, migration through blocking JAK1/STAT3 and Ras/Raf/MEK/ERK pathways in human colorectal carcinoma cells. |
| first_indexed | 2024-03-07T14:56:29Z |
| format | Article |
| id | doaj.art-6d1b1a59565942f18911be3b6bffdcad |
| institution | Directory Open Access Journal |
| issn | 1471-2407 |
| language | English |
| last_indexed | 2024-03-07T14:56:29Z |
| publishDate | 2019-11-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Cancer |
| spelling | doaj.art-6d1b1a59565942f18911be3b6bffdcad2024-03-05T19:24:27ZengBMCBMC Cancer1471-24072019-11-0119111010.1186/s12885-019-6381-yRETRACTED ARTICLE: Syndecan-1 suppresses cell growth and migration via blocking JAK1/STAT3 and Ras/Raf/MEK/ERK pathways in human colorectal carcinoma cellsShaojun Wang0Xiaofei Zhang1Guimei Wang2Bin Cao3Hong Yang4Lipeng Jin5Mingjuan Cui6Yongjun Mao7Department of Gastroenterology, The Affiliated Hospital of Qingdao UniversityDepartment of Gastroenterology, The Affiliated Hospital of Qingdao UniversityDepartment of Geriatrics, The Affiliated Hospital of Qingdao UniversityDepartment of Gastroenterology, The Affiliated Hospital of Qingdao UniversityEmergency Department, The Affiliated Hospital of Qingdao UniversityDepartment of Gastroenterology, The Affiliated Hospital of Qingdao UniversityDepartment of Gastroenterology, The Affiliated Hospital of Qingdao UniversityDepartment of Geriatrics, The Affiliated Hospital of Qingdao UniversityAbstract Background Syndecan-1 (SDC-1) is a crucial membrane proteoglycan, which is confirmed to participate in several tumor cell biological processes. However, the biological significance of SDC-1 in colorectal carcinoma is not yet clear. An objective of this study was to investigate the role of SDC-1 in colorectal carcinoma cells. Methods Expression of SDC-1 in colorectal carcinoma tissues was evaluated by Reverse transcription-quantitative real-time PCR (RT-qPCR) and western blot. After transfection with pcDNA3.1 or pc-SDC-1, the transfection efficiency was measured. Next, SW480, SW620 and LOVO cell viability, apoptosis, migration and adhesion were assessed to explore the effects of exogenous overexpressed SDC-1 on colorectal carcinoma. In addition, the influences of aberrant expressed SDC-1 in Janus kinase 1 (JAK1)/signal transducer and activator of transcription 3 (STAT3) and rat sarcoma virus (Ras)/rapidly accelerated fibrosarcoma (Raf)/mitogen-activated protein kinase (MEK)/extracellular signal-regulated kinase (ERK) pathways were detected by western blot analysis. Results SDC-1 mRNA and protein levels were down-regulated in human colorectal carcinoma tissues. SDC-1 overexpression inhibited cell proliferation via suppressing CyclinD1 and c-Myc expression, meanwhile stimulated cell apoptosis via increasing the expression levels of B-cell lymphoma-2-associated x (Bax) and Cleaved-Caspase-3. Additionally, SDC-1 overexpression restrained cell migration via inhibiting the protein expression of matrix metallopeptidase 9 (MMP-9), and elicited cell adhesion through increasing intercellular cell adhesion molecule-1 (ICAM-1). Furthermore, SDC-1 overexpression suppressed JAK1/STAT3 and Ras/Raf/MEK/ERK-related protein levels. Conclusions In general, the evidence from this study suggested that SDC-1 suppressed cell growth, migration through blocking JAK1/STAT3 and Ras/Raf/MEK/ERK pathways in human colorectal carcinoma cells.https://doi.org/10.1186/s12885-019-6381-ySyndecan-1Colorectal carcinomaMigrationJAK1/STAT3Ras/Raf/MEK/ERK |
| spellingShingle | Shaojun Wang Xiaofei Zhang Guimei Wang Bin Cao Hong Yang Lipeng Jin Mingjuan Cui Yongjun Mao RETRACTED ARTICLE: Syndecan-1 suppresses cell growth and migration via blocking JAK1/STAT3 and Ras/Raf/MEK/ERK pathways in human colorectal carcinoma cells BMC Cancer Syndecan-1 Colorectal carcinoma Migration JAK1/STAT3 Ras/Raf/MEK/ERK |
| title | RETRACTED ARTICLE: Syndecan-1 suppresses cell growth and migration via blocking JAK1/STAT3 and Ras/Raf/MEK/ERK pathways in human colorectal carcinoma cells |
| title_full | RETRACTED ARTICLE: Syndecan-1 suppresses cell growth and migration via blocking JAK1/STAT3 and Ras/Raf/MEK/ERK pathways in human colorectal carcinoma cells |
| title_fullStr | RETRACTED ARTICLE: Syndecan-1 suppresses cell growth and migration via blocking JAK1/STAT3 and Ras/Raf/MEK/ERK pathways in human colorectal carcinoma cells |
| title_full_unstemmed | RETRACTED ARTICLE: Syndecan-1 suppresses cell growth and migration via blocking JAK1/STAT3 and Ras/Raf/MEK/ERK pathways in human colorectal carcinoma cells |
| title_short | RETRACTED ARTICLE: Syndecan-1 suppresses cell growth and migration via blocking JAK1/STAT3 and Ras/Raf/MEK/ERK pathways in human colorectal carcinoma cells |
| title_sort | retracted article syndecan 1 suppresses cell growth and migration via blocking jak1 stat3 and ras raf mek erk pathways in human colorectal carcinoma cells |
| topic | Syndecan-1 Colorectal carcinoma Migration JAK1/STAT3 Ras/Raf/MEK/ERK |
| url | https://doi.org/10.1186/s12885-019-6381-y |
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