Polar protein Wag31 both activates and inhibits cell wall metabolism at the poles and septum
Mycobacterial cell elongation occurs at the cell poles; however, it is not clear how cell wall insertion is restricted to the pole or how it is organized. Wag31 is a pole-localized cytoplasmic protein that is essential for polar growth, but its molecular function has not been described. In this stud...
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| Format: | Article |
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Frontiers Media S.A.
2023-01-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1085918/full |
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| author | Neda Habibi Arejan Delfina Ensinck Lautaro Diacovich Parthvi Bharatkumar Patel Samantha Y. Quintanilla Arash Emami Saleh Hugo Gramajo Cara C. Boutte |
| author_facet | Neda Habibi Arejan Delfina Ensinck Lautaro Diacovich Parthvi Bharatkumar Patel Samantha Y. Quintanilla Arash Emami Saleh Hugo Gramajo Cara C. Boutte |
| author_sort | Neda Habibi Arejan |
| collection | DOAJ |
| description | Mycobacterial cell elongation occurs at the cell poles; however, it is not clear how cell wall insertion is restricted to the pole or how it is organized. Wag31 is a pole-localized cytoplasmic protein that is essential for polar growth, but its molecular function has not been described. In this study we used alanine scanning mutagenesis to identify Wag31 residues involved in cell morphogenesis. Our data show that Wag31 helps to control proper septation as well as new and old pole elongation. We have identified key amino acid residues involved in these essential functions. Enzyme assays revealed that Wag31 interacts with lipid metabolism by modulating acyl-CoA carboxylase (ACCase) activity. We show that Wag31 does not control polar growth by regulating the localization of cell wall precursor enzymes to the Intracellular Membrane Domain, and we also demonstrate that phosphorylation of Wag31 does not substantively regulate peptidoglycan metabolism. This work establishes new regulatory functions of Wag31 in the mycobacterial cell cycle and clarifies the need for new molecular models of Wag31 function. |
| first_indexed | 2024-04-10T23:30:38Z |
| format | Article |
| id | doaj.art-6d1e1ef288bf490a8139d4710e45fd32 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-10T23:30:38Z |
| publishDate | 2023-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-6d1e1ef288bf490a8139d4710e45fd322023-01-12T06:14:01ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-01-011310.3389/fmicb.2022.10859181085918Polar protein Wag31 both activates and inhibits cell wall metabolism at the poles and septumNeda Habibi Arejan0Delfina Ensinck1Lautaro Diacovich2Parthvi Bharatkumar Patel3Samantha Y. Quintanilla4Arash Emami Saleh5Hugo Gramajo6Cara C. Boutte7Department of Biology, University of Texas at Arlington, Arlington, TX, United StatesLaboratory of Physiology and Genetics of Actinomycetes, Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET), Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Rosario, ArgentinaLaboratory of Physiology and Genetics of Actinomycetes, Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET), Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Rosario, ArgentinaDepartment of Biology, University of Texas at Arlington, Arlington, TX, United StatesDepartment of Biology, University of Texas at Arlington, Arlington, TX, United StatesDepartment of Civil Engineering, University of Texas at Arlington, Arlington, TX, United StatesLaboratory of Physiology and Genetics of Actinomycetes, Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET), Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Rosario, ArgentinaDepartment of Biology, University of Texas at Arlington, Arlington, TX, United StatesMycobacterial cell elongation occurs at the cell poles; however, it is not clear how cell wall insertion is restricted to the pole or how it is organized. Wag31 is a pole-localized cytoplasmic protein that is essential for polar growth, but its molecular function has not been described. In this study we used alanine scanning mutagenesis to identify Wag31 residues involved in cell morphogenesis. Our data show that Wag31 helps to control proper septation as well as new and old pole elongation. We have identified key amino acid residues involved in these essential functions. Enzyme assays revealed that Wag31 interacts with lipid metabolism by modulating acyl-CoA carboxylase (ACCase) activity. We show that Wag31 does not control polar growth by regulating the localization of cell wall precursor enzymes to the Intracellular Membrane Domain, and we also demonstrate that phosphorylation of Wag31 does not substantively regulate peptidoglycan metabolism. This work establishes new regulatory functions of Wag31 in the mycobacterial cell cycle and clarifies the need for new molecular models of Wag31 function.https://www.frontiersin.org/articles/10.3389/fmicb.2022.1085918/fullMycobacteriumpolar growth regulationpetidoglycan synthesisDivIVA, intracellular membrane domain (IMD)MurGACCase complex |
| spellingShingle | Neda Habibi Arejan Delfina Ensinck Lautaro Diacovich Parthvi Bharatkumar Patel Samantha Y. Quintanilla Arash Emami Saleh Hugo Gramajo Cara C. Boutte Polar protein Wag31 both activates and inhibits cell wall metabolism at the poles and septum Frontiers in Microbiology Mycobacterium polar growth regulation petidoglycan synthesis DivIVA, intracellular membrane domain (IMD) MurG ACCase complex |
| title | Polar protein Wag31 both activates and inhibits cell wall metabolism at the poles and septum |
| title_full | Polar protein Wag31 both activates and inhibits cell wall metabolism at the poles and septum |
| title_fullStr | Polar protein Wag31 both activates and inhibits cell wall metabolism at the poles and septum |
| title_full_unstemmed | Polar protein Wag31 both activates and inhibits cell wall metabolism at the poles and septum |
| title_short | Polar protein Wag31 both activates and inhibits cell wall metabolism at the poles and septum |
| title_sort | polar protein wag31 both activates and inhibits cell wall metabolism at the poles and septum |
| topic | Mycobacterium polar growth regulation petidoglycan synthesis DivIVA, intracellular membrane domain (IMD) MurG ACCase complex |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1085918/full |
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