Persister <named-content content-type="genus-species">Escherichia coli</named-content> Cells Have a Lower Intracellular pH than Susceptible Cells but Maintain Their pH in Response to Antibiotic Treatment

Persister <named-content content-type="genus-species">Escherichia coli</named-content> Cells Have a Lower Intracellular pH than Susceptible Cells but Maintain Their pH in Response to Antibiotic Treatment

ABSTRACT Persister and viable but non-culturable (VBNC) cells are two clonal subpopulations that can survive multidrug exposure via a plethora of putative molecular mechanisms. Here, we combine microfluidics, time-lapse microscopy, and a plasmid-encoded fluorescent pH reporter to measure the dynamic...

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Bibliographic Details
Main Authors: Olivia Goode, Ashley Smith, Ashraf Zarkan, Jehangir Cama, Brandon M. Invergo, Daaniyah Belgami, Santiago Caño-Muñiz, Jeremy Metz, Paul O’Neill, Aaron Jeffries, Isobel H. Norville, Jonathan David, David Summers, Stefano Pagliara
Format: Article
Language:English
Published: American Society for Microbiology 2021-08-01
Series:mBio
Subjects:
indole
intracellular pH
microfluidics
single-cell analysis
antibiotic resistance
antibiotics
Online Access:https://journals.asm.org/doi/10.1128/mBio.00909-21

Internet

https://journals.asm.org/doi/10.1128/mBio.00909-21

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