Reduction of phenolics in faba bean meal using recombinantly produced and purified Bacillus ligniniphilus catechol 2,3-dioxygenase
Abstract Pulse meal should be a valuable product in the animal feed industry based on its strong nutritional and protein profiles. However, it contains anti-nutritional compounds including phenolics (large and small molecular weight), which must be addressed to increase uptake by the industry. Micro...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SpringerOpen
2023-02-01
|
| Series: | Bioresources and Bioprocessing |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s40643-023-00633-8 |
| _version_ | 1797865674718052352 |
|---|---|
| author | Rebecca M. Murphy Joanna C. Stanczyk Fang Huang Matthew E. Loewen Trent C. Yang Michele C. Loewen |
| author_facet | Rebecca M. Murphy Joanna C. Stanczyk Fang Huang Matthew E. Loewen Trent C. Yang Michele C. Loewen |
| author_sort | Rebecca M. Murphy |
| collection | DOAJ |
| description | Abstract Pulse meal should be a valuable product in the animal feed industry based on its strong nutritional and protein profiles. However, it contains anti-nutritional compounds including phenolics (large and small molecular weight), which must be addressed to increase uptake by the industry. Microbial fermentation is currently used as a strategy to decrease larger molecular weight poly-phenolics, but results in the undesirable accumulation of small mono-phenolics. Here, we investigate cell-free biocatalytic reduction of phenolic content in faba bean (Vicia faba L.) meal. A representative phenolic ring-breaking catechol dioxygenase, Bacillus ligniniphilus L1 catechol 2,3-dioxygenase (BLC23O) was used in this proof-of concept based on its known stability and broad substrate specificity. Initially, large-scale fermentative recombinant production and purification of BLC23O was carried out, with functionality validated by in vitro kinetic analysis. When applied to faba bean meal, BLC23O yielded greatest reductions in phenolic content in a coarse air classified fraction (high carbohydrate), compared to either a fine fraction (high protein) or the original unfractionated meal. However, the upstream hydrolytic release of phenolics from higher molecular weight species (e.g. tannins, or complexes with proteins and carbohydrates) likely remains a rate limiting step, in the absence of other enzymes or microbial fermentation. Consistent with this, when applied to a selection of commercially available purified phenolic compounds, known to occur in faba bean, BLC23O was found to have high activity against monophenolic acids and little if any detectable activity against larger molecular weight compounds. Overall, this study highlights the potential viability of the biocatalytic processing of pulse meals, for optimization of their nutritional and economical value in the animal feed industry. Graphical Abstract |
| first_indexed | 2024-04-09T23:13:06Z |
| format | Article |
| id | doaj.art-6d2022b05fc6440ba29e2fd5a70b34f5 |
| institution | Directory Open Access Journal |
| issn | 2197-4365 |
| language | English |
| last_indexed | 2024-04-09T23:13:06Z |
| publishDate | 2023-02-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Bioresources and Bioprocessing |
| spelling | doaj.art-6d2022b05fc6440ba29e2fd5a70b34f52023-03-22T10:17:50ZengSpringerOpenBioresources and Bioprocessing2197-43652023-02-0110111210.1186/s40643-023-00633-8Reduction of phenolics in faba bean meal using recombinantly produced and purified Bacillus ligniniphilus catechol 2,3-dioxygenaseRebecca M. Murphy0Joanna C. Stanczyk1Fang Huang2Matthew E. Loewen3Trent C. Yang4Michele C. Loewen5Department of Chemistry and Biomolecular Sciences, University of OttawaAquatic and Crop Resources Development Research Center, National Research Council of CanadaAquatic and Crop Resources Development Research Center, National Research Council of CanadaDepartment of Veterinary Biomedical Sciences, Western College of Veterinary Medicine, University of SaskatchewanAquatic and Crop Resources Development Research Center, National Research Council of CanadaDepartment of Chemistry and Biomolecular Sciences, University of OttawaAbstract Pulse meal should be a valuable product in the animal feed industry based on its strong nutritional and protein profiles. However, it contains anti-nutritional compounds including phenolics (large and small molecular weight), which must be addressed to increase uptake by the industry. Microbial fermentation is currently used as a strategy to decrease larger molecular weight poly-phenolics, but results in the undesirable accumulation of small mono-phenolics. Here, we investigate cell-free biocatalytic reduction of phenolic content in faba bean (Vicia faba L.) meal. A representative phenolic ring-breaking catechol dioxygenase, Bacillus ligniniphilus L1 catechol 2,3-dioxygenase (BLC23O) was used in this proof-of concept based on its known stability and broad substrate specificity. Initially, large-scale fermentative recombinant production and purification of BLC23O was carried out, with functionality validated by in vitro kinetic analysis. When applied to faba bean meal, BLC23O yielded greatest reductions in phenolic content in a coarse air classified fraction (high carbohydrate), compared to either a fine fraction (high protein) or the original unfractionated meal. However, the upstream hydrolytic release of phenolics from higher molecular weight species (e.g. tannins, or complexes with proteins and carbohydrates) likely remains a rate limiting step, in the absence of other enzymes or microbial fermentation. Consistent with this, when applied to a selection of commercially available purified phenolic compounds, known to occur in faba bean, BLC23O was found to have high activity against monophenolic acids and little if any detectable activity against larger molecular weight compounds. Overall, this study highlights the potential viability of the biocatalytic processing of pulse meals, for optimization of their nutritional and economical value in the animal feed industry. Graphical Abstracthttps://doi.org/10.1186/s40643-023-00633-8Pulse mealVicia fabaBiocatalysisCatechol 2,3 dioxygenasePhenol reduction |
| spellingShingle | Rebecca M. Murphy Joanna C. Stanczyk Fang Huang Matthew E. Loewen Trent C. Yang Michele C. Loewen Reduction of phenolics in faba bean meal using recombinantly produced and purified Bacillus ligniniphilus catechol 2,3-dioxygenase Bioresources and Bioprocessing Pulse meal Vicia faba Biocatalysis Catechol 2,3 dioxygenase Phenol reduction |
| title | Reduction of phenolics in faba bean meal using recombinantly produced and purified Bacillus ligniniphilus catechol 2,3-dioxygenase |
| title_full | Reduction of phenolics in faba bean meal using recombinantly produced and purified Bacillus ligniniphilus catechol 2,3-dioxygenase |
| title_fullStr | Reduction of phenolics in faba bean meal using recombinantly produced and purified Bacillus ligniniphilus catechol 2,3-dioxygenase |
| title_full_unstemmed | Reduction of phenolics in faba bean meal using recombinantly produced and purified Bacillus ligniniphilus catechol 2,3-dioxygenase |
| title_short | Reduction of phenolics in faba bean meal using recombinantly produced and purified Bacillus ligniniphilus catechol 2,3-dioxygenase |
| title_sort | reduction of phenolics in faba bean meal using recombinantly produced and purified bacillus ligniniphilus catechol 2 3 dioxygenase |
| topic | Pulse meal Vicia faba Biocatalysis Catechol 2,3 dioxygenase Phenol reduction |
| url | https://doi.org/10.1186/s40643-023-00633-8 |
| work_keys_str_mv | AT rebeccammurphy reductionofphenolicsinfababeanmealusingrecombinantlyproducedandpurifiedbacillusligniniphiluscatechol23dioxygenase AT joannacstanczyk reductionofphenolicsinfababeanmealusingrecombinantlyproducedandpurifiedbacillusligniniphiluscatechol23dioxygenase AT fanghuang reductionofphenolicsinfababeanmealusingrecombinantlyproducedandpurifiedbacillusligniniphiluscatechol23dioxygenase AT mattheweloewen reductionofphenolicsinfababeanmealusingrecombinantlyproducedandpurifiedbacillusligniniphiluscatechol23dioxygenase AT trentcyang reductionofphenolicsinfababeanmealusingrecombinantlyproducedandpurifiedbacillusligniniphiluscatechol23dioxygenase AT michelecloewen reductionofphenolicsinfababeanmealusingrecombinantlyproducedandpurifiedbacillusligniniphiluscatechol23dioxygenase |