Role of Transient Receptor Potential Vanilloid 1 in Sonic Hedgehog-Dependent Taste Bud Differentiation
Taste bud cell differentiation is extremely important for taste sensation. Immature taste bud cells cannot function during taste perception transmission to the nerve. In this study, we investigated whether hedgehog signaling affected taste bud cell differentiation and whether transient receptor pote...
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MDPI AG
2022-12-01
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| Online Access: | https://www.mdpi.com/2075-1729/13/1/75 |
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| author | Yun-Hee Rhee Young-Hoon Choi Allison C. Hu Min Young Lee Jin-Chul Ahn Sehwan Kim Ji-Hun Mo Seung Hoon Woo Phil-Sang Chung |
| author_facet | Yun-Hee Rhee Young-Hoon Choi Allison C. Hu Min Young Lee Jin-Chul Ahn Sehwan Kim Ji-Hun Mo Seung Hoon Woo Phil-Sang Chung |
| author_sort | Yun-Hee Rhee |
| collection | DOAJ |
| description | Taste bud cell differentiation is extremely important for taste sensation. Immature taste bud cells cannot function during taste perception transmission to the nerve. In this study, we investigated whether hedgehog signaling affected taste bud cell differentiation and whether transient receptor potential vanilloid 1 (TRPV1) played a key role in dry mouth. The induction of dry mouth due to salivary gland resection (SGR) was confirmed on the basis of reduced salivation and disrupted fungiform papillae. The expression of keratin 8 (K8) of taste bud cells, neurofilament (NF), sonic hedgehog (Shh), and glioma-associated oncogene homolog 1 (Gli1) around taste bud cells was downregulated; however, the expression of TRPV1, P2X purinoceptor 3 (P2X3), and hematopoietic stem cell factor (c-Kit) was upregulated at the NF ends in the dry mouth group. To investigate the effect of TRPV1 defect on dry mouth, we induced dry mouth in the TRPV-/- group. The K8, NF, and P2X3 expression patterns were the same in the TRPV1 wild-type and TRPV1-/- dry mouth groups. However, Shh and c-Kit expression decreased regardless of dry mouth in the case of TRPV1 deficiency. These results indicated that TRPV1 positively regulated proliferation during taste bud cell injury by blocking the Shh/Gli1 pathway. In addition, not only cell proliferation but also differentiation of taste bud cells could not be regulated under TRPV1-deficiency conditions. Thus, TRPV1 positively regulates taste bud cell innervation and differentiation; this finding could be valuable in the clinical treatment of dry mouth-related taste dysfunction. |
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| last_indexed | 2024-03-09T11:57:39Z |
| publishDate | 2022-12-01 |
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| series | Life |
| spelling | doaj.art-6d20761f7e864ee2abb63f71a6c4d4a62023-11-30T23:07:41ZengMDPI AGLife2075-17292022-12-011317510.3390/life13010075Role of Transient Receptor Potential Vanilloid 1 in Sonic Hedgehog-Dependent Taste Bud DifferentiationYun-Hee Rhee0Young-Hoon Choi1Allison C. Hu2Min Young Lee3Jin-Chul Ahn4Sehwan Kim5Ji-Hun Mo6Seung Hoon Woo7Phil-Sang Chung8Beckman Laser Institute Korea, Dankook University, 119 Dandae-ro, Cheonan 31116, Republic of KoreaLaser Translational Clinical Trial Center, Dankook University Hospital, Cheonan 31116, Republic of KoreaBeckman Laser Institute and Medical Clinic, University of California Irvine, 1002 Health Sciences Rd., Irvine, CA 92697, USABeckman Laser Institute Korea, Dankook University, 119 Dandae-ro, Cheonan 31116, Republic of KoreaBeckman Laser Institute Korea, Dankook University, 119 Dandae-ro, Cheonan 31116, Republic of KoreaBeckman Laser Institute Korea, Dankook University, 119 Dandae-ro, Cheonan 31116, Republic of KoreaBeckman Laser Institute Korea, Dankook University, 119 Dandae-ro, Cheonan 31116, Republic of KoreaBeckman Laser Institute Korea, Dankook University, 119 Dandae-ro, Cheonan 31116, Republic of KoreaBeckman Laser Institute Korea, Dankook University, 119 Dandae-ro, Cheonan 31116, Republic of KoreaTaste bud cell differentiation is extremely important for taste sensation. Immature taste bud cells cannot function during taste perception transmission to the nerve. In this study, we investigated whether hedgehog signaling affected taste bud cell differentiation and whether transient receptor potential vanilloid 1 (TRPV1) played a key role in dry mouth. The induction of dry mouth due to salivary gland resection (SGR) was confirmed on the basis of reduced salivation and disrupted fungiform papillae. The expression of keratin 8 (K8) of taste bud cells, neurofilament (NF), sonic hedgehog (Shh), and glioma-associated oncogene homolog 1 (Gli1) around taste bud cells was downregulated; however, the expression of TRPV1, P2X purinoceptor 3 (P2X3), and hematopoietic stem cell factor (c-Kit) was upregulated at the NF ends in the dry mouth group. To investigate the effect of TRPV1 defect on dry mouth, we induced dry mouth in the TRPV-/- group. The K8, NF, and P2X3 expression patterns were the same in the TRPV1 wild-type and TRPV1-/- dry mouth groups. However, Shh and c-Kit expression decreased regardless of dry mouth in the case of TRPV1 deficiency. These results indicated that TRPV1 positively regulated proliferation during taste bud cell injury by blocking the Shh/Gli1 pathway. In addition, not only cell proliferation but also differentiation of taste bud cells could not be regulated under TRPV1-deficiency conditions. Thus, TRPV1 positively regulates taste bud cell innervation and differentiation; this finding could be valuable in the clinical treatment of dry mouth-related taste dysfunction.https://www.mdpi.com/2075-1729/13/1/75taste bud cellsinnervationtransient receptor potential vanilloid 1sonic hedgehog |
| spellingShingle | Yun-Hee Rhee Young-Hoon Choi Allison C. Hu Min Young Lee Jin-Chul Ahn Sehwan Kim Ji-Hun Mo Seung Hoon Woo Phil-Sang Chung Role of Transient Receptor Potential Vanilloid 1 in Sonic Hedgehog-Dependent Taste Bud Differentiation Life taste bud cells innervation transient receptor potential vanilloid 1 sonic hedgehog |
| title | Role of Transient Receptor Potential Vanilloid 1 in Sonic Hedgehog-Dependent Taste Bud Differentiation |
| title_full | Role of Transient Receptor Potential Vanilloid 1 in Sonic Hedgehog-Dependent Taste Bud Differentiation |
| title_fullStr | Role of Transient Receptor Potential Vanilloid 1 in Sonic Hedgehog-Dependent Taste Bud Differentiation |
| title_full_unstemmed | Role of Transient Receptor Potential Vanilloid 1 in Sonic Hedgehog-Dependent Taste Bud Differentiation |
| title_short | Role of Transient Receptor Potential Vanilloid 1 in Sonic Hedgehog-Dependent Taste Bud Differentiation |
| title_sort | role of transient receptor potential vanilloid 1 in sonic hedgehog dependent taste bud differentiation |
| topic | taste bud cells innervation transient receptor potential vanilloid 1 sonic hedgehog |
| url | https://www.mdpi.com/2075-1729/13/1/75 |
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