Production of Poly-α-hydroxybutyrate (PHB) from Sago Starch by The Native Isolate Bacillus megaterium PSA10
A new bacterial strain that produces amylase and poly-α-hidroxybutyrate (PHB) using sago starch as carbon source was characterized and identified to be member of the Bacillus megaterium group based on phenotypic characteristics and 16S rDNA gene sequences. Amylase activity was determined s...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Universitas Gadjah Mada, Yogyakarta
2006-06-01
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| Series: | Indonesian Journal of Biotechnology |
| Online Access: | https://jurnal.ugm.ac.id/ijbiotech/article/view/7559 |
| _version_ | 1818322869493432320 |
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| author | Nur Arfa Yanti Langkah Sembiring Sebastian Margino |
| author_facet | Nur Arfa Yanti Langkah Sembiring Sebastian Margino |
| author_sort | Nur Arfa Yanti |
| collection | DOAJ |
| description | A new bacterial strain that produces amylase and poly-α-hidroxybutyrate (PHB) using sago starch as carbon source was characterized and identified to be member of the Bacillus megaterium group based on phenotypic characteristics and 16S rDNA gene sequences. Amylase activity was determined spectrophotometrically on the basis of substrate concentration reduction. PHB production was quantified with UV spectrophotometer. The polymer produced by B. megaterium PSA10 was identified by Fourier Transform Infrared spectroscopy (FTIR). The result of the study showed that the amylase specific activity B. megaterium PSA10 was 593,61 DUN/mg protein and PHB production from sago starch was 52,28 % of cell dry weight (CDW). FTIR analysis of the polymer indicated that the strain B.megaterium PSA10 was a potent PHB producer. |
| first_indexed | 2024-12-13T11:03:39Z |
| format | Article |
| id | doaj.art-6d210ad9ff94474ea44e66d4cae1db25 |
| institution | Directory Open Access Journal |
| issn | 0853-8654 2089-2241 |
| language | English |
| last_indexed | 2024-12-13T11:03:39Z |
| publishDate | 2006-06-01 |
| publisher | Universitas Gadjah Mada, Yogyakarta |
| record_format | Article |
| series | Indonesian Journal of Biotechnology |
| spelling | doaj.art-6d210ad9ff94474ea44e66d4cae1db252022-12-21T23:49:09ZengUniversitas Gadjah Mada, YogyakartaIndonesian Journal of Biotechnology0853-86542089-22412006-06-0111110.22146/ijbiotech.75596360Production of Poly-α-hydroxybutyrate (PHB) from Sago Starch by The Native Isolate Bacillus megaterium PSA10Nur Arfa YantiLangkah SembiringSebastian MarginoA new bacterial strain that produces amylase and poly-α-hidroxybutyrate (PHB) using sago starch as carbon source was characterized and identified to be member of the Bacillus megaterium group based on phenotypic characteristics and 16S rDNA gene sequences. Amylase activity was determined spectrophotometrically on the basis of substrate concentration reduction. PHB production was quantified with UV spectrophotometer. The polymer produced by B. megaterium PSA10 was identified by Fourier Transform Infrared spectroscopy (FTIR). The result of the study showed that the amylase specific activity B. megaterium PSA10 was 593,61 DUN/mg protein and PHB production from sago starch was 52,28 % of cell dry weight (CDW). FTIR analysis of the polymer indicated that the strain B.megaterium PSA10 was a potent PHB producer.https://jurnal.ugm.ac.id/ijbiotech/article/view/7559 |
| spellingShingle | Nur Arfa Yanti Langkah Sembiring Sebastian Margino Production of Poly-α-hydroxybutyrate (PHB) from Sago Starch by The Native Isolate Bacillus megaterium PSA10 Indonesian Journal of Biotechnology |
| title | Production of Poly-α-hydroxybutyrate (PHB) from Sago Starch by The Native Isolate Bacillus megaterium PSA10 |
| title_full | Production of Poly-α-hydroxybutyrate (PHB) from Sago Starch by The Native Isolate Bacillus megaterium PSA10 |
| title_fullStr | Production of Poly-α-hydroxybutyrate (PHB) from Sago Starch by The Native Isolate Bacillus megaterium PSA10 |
| title_full_unstemmed | Production of Poly-α-hydroxybutyrate (PHB) from Sago Starch by The Native Isolate Bacillus megaterium PSA10 |
| title_short | Production of Poly-α-hydroxybutyrate (PHB) from Sago Starch by The Native Isolate Bacillus megaterium PSA10 |
| title_sort | production of poly α hydroxybutyrate phb from sago starch by the native isolate bacillus megaterium psa10 |
| url | https://jurnal.ugm.ac.id/ijbiotech/article/view/7559 |
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