NtNAC053, A Novel NAC Transcription Factor, Confers Drought and Salt Tolerances in Tobacco
The NAC (NAM, ATAF1/2, and CUC2) family acts as one of the largest families of the transcription factor in the plant kingdom and was revealed to function as the important regulators in various environmental stresses. However, a few studies were reported about the biofunctions of the NAC transcriptio...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-05-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.817106/full |
| _version_ | 1818274310692798464 |
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| author | Xiaoxu Li Xiaoxu Li Qi Wang Qi Wang Cun Guo Cun Guo Jinhao Sun Zhiyuan Li Yaofu Wang Aiguo Yang Wenxuan Pu Yongfeng Guo Junping Gao Liuying Wen |
| author_facet | Xiaoxu Li Xiaoxu Li Qi Wang Qi Wang Cun Guo Cun Guo Jinhao Sun Zhiyuan Li Yaofu Wang Aiguo Yang Wenxuan Pu Yongfeng Guo Junping Gao Liuying Wen |
| author_sort | Xiaoxu Li |
| collection | DOAJ |
| description | The NAC (NAM, ATAF1/2, and CUC2) family acts as one of the largest families of the transcription factor in the plant kingdom and was revealed to function as the important regulators in various environmental stresses. However, a few studies were reported about the biofunctions of the NAC transcription factor in tobacco. In the current study, we characterized a novel NAC transcription factor encoding the gene NtNAC053 in tobacco, which was significantly up-regulated when exposed to salt and drought treatments. The results of cis-acting elements analysis suggested that the promoter region of NtNAC053 gene possesses a number of stress-responsive elements, and this gene could be induced by exogenous abscisic acid (ABA) treatment. Moreover, the NtNAC053–GFP fusion protein was localized in the cell nucleus and possessed a transactivation domain in its C-terminal, implying that NtNAC053 may undertake as a transcriptional activator in tobacco. Notably, the overexpression of NtNAC053 in tobacco resulted in hypersensitivity to ABA treatment. Furthermore, these overexpression lines showed significantly enhanced tolerances to drought and salt stresses. Under salt and drought stresses, these overexpression lines possessed higher superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD) activities. Interestingly, the expressions of putative stress-related genes, including NtCOR15A, NtRAB18, NtDREB1A, NtERF5, NtKAT2, and NtERD11, were up-regulated in these overexpression lines when subjected to salt and drought stresses. The clues provided in our study suggested that the NtNAC053 gene encodes a novel NAC transcription factor and could confer the drought and salt stress tolerances by inspiring the downstream stress-responsive genes and antioxidant system in tobacco. |
| first_indexed | 2024-12-12T22:11:50Z |
| format | Article |
| id | doaj.art-6d21346280894be4a31693c3967b9cd4 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-12T22:11:50Z |
| publishDate | 2022-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-6d21346280894be4a31693c3967b9cd42022-12-22T00:10:13ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-05-011310.3389/fpls.2022.817106817106NtNAC053, A Novel NAC Transcription Factor, Confers Drought and Salt Tolerances in TobaccoXiaoxu Li0Xiaoxu Li1Qi Wang2Qi Wang3Cun Guo4Cun Guo5Jinhao Sun6Zhiyuan Li7Yaofu Wang8Aiguo Yang9Wenxuan Pu10Yongfeng Guo11Junping Gao12Liuying Wen13Technology Center, China Tobacco Hunan Industrial Co., Ltd., Changsha, ChinaKey Laboratory for Tobacco Gene Resources, Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao, ChinaKey Laboratory for Tobacco Gene Resources, Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao, ChinaShandong Peanut Research Institute, Qingdao, ChinaKey Laboratory for Tobacco Gene Resources, Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao, ChinaGraduate School of Chinese Academy of Agricultural Sciences, Beijing, ChinaKey Laboratory for Tobacco Gene Resources, Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao, ChinaKey Laboratory for Tobacco Gene Resources, Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao, ChinaTechnology Center, China Tobacco Hunan Industrial Co., Ltd., Changsha, ChinaKey Laboratory for Tobacco Gene Resources, Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao, ChinaTechnology Center, China Tobacco Hunan Industrial Co., Ltd., Changsha, ChinaKey Laboratory for Tobacco Gene Resources, Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao, ChinaTechnology Center, China Tobacco Hunan Industrial Co., Ltd., Changsha, ChinaKey Laboratory for Tobacco Gene Resources, Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao, ChinaThe NAC (NAM, ATAF1/2, and CUC2) family acts as one of the largest families of the transcription factor in the plant kingdom and was revealed to function as the important regulators in various environmental stresses. However, a few studies were reported about the biofunctions of the NAC transcription factor in tobacco. In the current study, we characterized a novel NAC transcription factor encoding the gene NtNAC053 in tobacco, which was significantly up-regulated when exposed to salt and drought treatments. The results of cis-acting elements analysis suggested that the promoter region of NtNAC053 gene possesses a number of stress-responsive elements, and this gene could be induced by exogenous abscisic acid (ABA) treatment. Moreover, the NtNAC053–GFP fusion protein was localized in the cell nucleus and possessed a transactivation domain in its C-terminal, implying that NtNAC053 may undertake as a transcriptional activator in tobacco. Notably, the overexpression of NtNAC053 in tobacco resulted in hypersensitivity to ABA treatment. Furthermore, these overexpression lines showed significantly enhanced tolerances to drought and salt stresses. Under salt and drought stresses, these overexpression lines possessed higher superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD) activities. Interestingly, the expressions of putative stress-related genes, including NtCOR15A, NtRAB18, NtDREB1A, NtERF5, NtKAT2, and NtERD11, were up-regulated in these overexpression lines when subjected to salt and drought stresses. The clues provided in our study suggested that the NtNAC053 gene encodes a novel NAC transcription factor and could confer the drought and salt stress tolerances by inspiring the downstream stress-responsive genes and antioxidant system in tobacco.https://www.frontiersin.org/articles/10.3389/fpls.2022.817106/fulltobaccoNtNAC053ABAabiotic stressantioxidant system |
| spellingShingle | Xiaoxu Li Xiaoxu Li Qi Wang Qi Wang Cun Guo Cun Guo Jinhao Sun Zhiyuan Li Yaofu Wang Aiguo Yang Wenxuan Pu Yongfeng Guo Junping Gao Liuying Wen NtNAC053, A Novel NAC Transcription Factor, Confers Drought and Salt Tolerances in Tobacco Frontiers in Plant Science tobacco NtNAC053 ABA abiotic stress antioxidant system |
| title | NtNAC053, A Novel NAC Transcription Factor, Confers Drought and Salt Tolerances in Tobacco |
| title_full | NtNAC053, A Novel NAC Transcription Factor, Confers Drought and Salt Tolerances in Tobacco |
| title_fullStr | NtNAC053, A Novel NAC Transcription Factor, Confers Drought and Salt Tolerances in Tobacco |
| title_full_unstemmed | NtNAC053, A Novel NAC Transcription Factor, Confers Drought and Salt Tolerances in Tobacco |
| title_short | NtNAC053, A Novel NAC Transcription Factor, Confers Drought and Salt Tolerances in Tobacco |
| title_sort | ntnac053 a novel nac transcription factor confers drought and salt tolerances in tobacco |
| topic | tobacco NtNAC053 ABA abiotic stress antioxidant system |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.817106/full |
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