miRNA expression differentiation induced by polyploidization in newly formed triploids of black poplar
During whole genomic duplication (WGD) events, micro RNAs (miRNAs) are involved in stabilization of chromatin and genome and epigenetic regulation of gene expression. In this study, a newly induced triploid group of hybrids between sect. Tacamahaca and sect. Aigeiros in Populus, was characterized f...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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AcademicPres
2022-12-01
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| Series: | Notulae Botanicae Horti Agrobotanici Cluj-Napoca |
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| Online Access: | https://www.notulaebotanicae.ro/index.php/nbha/article/view/12964 |
| _version_ | 1811198545432150016 |
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| author | Wenting XU Xue WANG Huichun LIU Jiaqiang ZHANG Jianghua ZHOU Rongxin GOU Kaiyuan ZHU |
| author_facet | Wenting XU Xue WANG Huichun LIU Jiaqiang ZHANG Jianghua ZHOU Rongxin GOU Kaiyuan ZHU |
| author_sort | Wenting XU |
| collection | DOAJ |
| description |
During whole genomic duplication (WGD) events, micro RNAs (miRNAs) are involved in stabilization of chromatin and genome and epigenetic regulation of gene expression. In this study, a newly induced triploid group of hybrids between sect. Tacamahaca and sect. Aigeiros in Populus, was characterized for genome-wide miRNA expression after WGD. Seven miRNA libraries (male parent, female parent, group of triploid offspring’s, group of diploid offspring, and three triploid individuals) were constructed and variation of miRNA expression from diploid parents to triploid offspring’s as well as distinction between triploid and diploid offspring were analyzed. The results showed that a total of 240 miRNAs were predicted including 187 known miRNAs and 53 novel miRNAs. 81.25% of miRNAs in triploid offspring were non-additively expressed in which 52.31% were down-regulated. A novel miRNA with 24nt in length choosing adenine as its first base was found in triploid offspring group suggesting its potential role in regulation of DNA methylation after WGD. A total of 18 novel miRNAs were specifically expressed in the library of triploid group. Targeted genes of different expressed miRNAs in three comparison sets (triploid offspring group vs female parent, male parent, and diploid offspring group) were all enriched in ADP binding (GO: 0043531; FDR < 0.05). KEGG enrichment pathway of all three comparison sets was plant-pathogen interaction. This study revealed an essential role of miRNAs involving in epigenetic regulation after WGD in poplar and provided a good model for further studies of polyploidization advantages in woody plant.
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| format | Article |
| id | doaj.art-6d2195d1d71644bc867fa4948ebdb4b4 |
| institution | Directory Open Access Journal |
| issn | 0255-965X 1842-4309 |
| language | English |
| last_indexed | 2024-04-12T01:33:10Z |
| publishDate | 2022-12-01 |
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| series | Notulae Botanicae Horti Agrobotanici Cluj-Napoca |
| spelling | doaj.art-6d2195d1d71644bc867fa4948ebdb4b42022-12-22T03:53:25ZengAcademicPresNotulae Botanicae Horti Agrobotanici Cluj-Napoca0255-965X1842-43092022-12-0150410.15835/nbha50312964miRNA expression differentiation induced by polyploidization in newly formed triploids of black poplarWenting XU0Xue WANG1Huichun LIU2Jiaqiang ZHANG3Jianghua ZHOU4Rongxin GOU5Kaiyuan ZHU6Zhejiang Institute of Landscape Plants and Flowers, Zhejiang Academy of Agricultural Sciences, Hangzhou 311251; Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, Key Laboratory of Forest Trees and Ornamental Plants biological engineering of State Forestry Administration, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083Institute of Grassland, Flowers and Ecology, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100097Zhejiang Institute of Landscape Plants and Flowers, Zhejiang Academy of Agricultural Sciences, Hangzhou 311251Zhejiang Institute of Landscape Plants and Flowers, Zhejiang Academy of Agricultural Sciences, Hangzhou 311251Zhejiang Institute of Landscape Plants and Flowers, Zhejiang Academy of Agricultural Sciences, Hangzhou 311251Institute of Grassland, Flowers and Ecology, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100097Zhejiang Institute of Landscape Plants and Flowers, Zhejiang Academy of Agricultural Sciences, Hangzhou 311251 During whole genomic duplication (WGD) events, micro RNAs (miRNAs) are involved in stabilization of chromatin and genome and epigenetic regulation of gene expression. In this study, a newly induced triploid group of hybrids between sect. Tacamahaca and sect. Aigeiros in Populus, was characterized for genome-wide miRNA expression after WGD. Seven miRNA libraries (male parent, female parent, group of triploid offspring’s, group of diploid offspring, and three triploid individuals) were constructed and variation of miRNA expression from diploid parents to triploid offspring’s as well as distinction between triploid and diploid offspring were analyzed. The results showed that a total of 240 miRNAs were predicted including 187 known miRNAs and 53 novel miRNAs. 81.25% of miRNAs in triploid offspring were non-additively expressed in which 52.31% were down-regulated. A novel miRNA with 24nt in length choosing adenine as its first base was found in triploid offspring group suggesting its potential role in regulation of DNA methylation after WGD. A total of 18 novel miRNAs were specifically expressed in the library of triploid group. Targeted genes of different expressed miRNAs in three comparison sets (triploid offspring group vs female parent, male parent, and diploid offspring group) were all enriched in ADP binding (GO: 0043531; FDR < 0.05). KEGG enrichment pathway of all three comparison sets was plant-pathogen interaction. This study revealed an essential role of miRNAs involving in epigenetic regulation after WGD in poplar and provided a good model for further studies of polyploidization advantages in woody plant. https://www.notulaebotanicae.ro/index.php/nbha/article/view/12964miRNAnewly formed triploidpoplar polyploidization |
| spellingShingle | Wenting XU Xue WANG Huichun LIU Jiaqiang ZHANG Jianghua ZHOU Rongxin GOU Kaiyuan ZHU miRNA expression differentiation induced by polyploidization in newly formed triploids of black poplar Notulae Botanicae Horti Agrobotanici Cluj-Napoca miRNA newly formed triploid poplar polyploidization |
| title | miRNA expression differentiation induced by polyploidization in newly formed triploids of black poplar |
| title_full | miRNA expression differentiation induced by polyploidization in newly formed triploids of black poplar |
| title_fullStr | miRNA expression differentiation induced by polyploidization in newly formed triploids of black poplar |
| title_full_unstemmed | miRNA expression differentiation induced by polyploidization in newly formed triploids of black poplar |
| title_short | miRNA expression differentiation induced by polyploidization in newly formed triploids of black poplar |
| title_sort | mirna expression differentiation induced by polyploidization in newly formed triploids of black poplar |
| topic | miRNA newly formed triploid poplar polyploidization |
| url | https://www.notulaebotanicae.ro/index.php/nbha/article/view/12964 |
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