Expression of multidrug-resistance associated proteins in human retinoblastoma treated by primary enucleation
AIM: To reveal the expression of multidrug-resistance associated proteins: glutathione-S-transferase π (GSTπ), P-glycoprotein (P-gp) and vault protein lung resistance protein (LRP) in retinoblastoma (RB) without any conservative treatment before primary enucleation and to correlate this expression w...
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| Format: | Article |
| Language: | English |
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Press of International Journal of Ophthalmology (IJO PRESS)
2018-09-01
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| Series: | International Journal of Ophthalmology |
| Subjects: | |
| Online Access: | http://www.ijo.cn/en_publish/2018/9/20180906.pdf |
| _version_ | 1818344718317125632 |
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| author | Li-Juan Tang Li-Jun Zhou Wen-Xin Zhang Jian-Yan Lin Yong-Ping Li Hua-Sheng Yang Ping Zhang |
| author_facet | Li-Juan Tang Li-Jun Zhou Wen-Xin Zhang Jian-Yan Lin Yong-Ping Li Hua-Sheng Yang Ping Zhang |
| author_sort | Li-Juan Tang |
| collection | DOAJ |
| description | AIM: To reveal the expression of multidrug-resistance associated proteins: glutathione-S-transferase π (GSTπ), P-glycoprotein (P-gp) and vault protein lung resistance protein (LRP) in retinoblastoma (RB) without any conservative treatment before primary enucleation and to correlate this expression with histopathological tumor features.
METHODS: A total of 42 specimens of RB undergone primary enucleation were selected for the research. Sections from the formalin-fixed, paraffin-embedded specimens were stained with HE and immunohistochemistry to detect the expression of GSTπ, P-gp and LRP.
RESULTS: GSTπ was expressed in 39/42 (92.86%) RBs and in 9/9 (100%) well-differentiated RBs. P-gp/GSTπ was found in 30 (71.42%) of 42 RBs. Totally 9 (21.43%) tumors were well differentiated and 33 (78.57%) were poorly differentiated. Totally 15 (35.71%) eyes had optic nerve (ON) tumor invasion, 36 (85.71%) had choroidal tumor invasion, and 14 (33.33%) had simultaneous choroidal and ON invasion. There was no statistically significant relationship between P-gp, GSTπ, LRP positivity and the degree of ocular layer tumor invasion and ON tumor invasion (P>0.05).
CONCLUSION: RB intrinsically expresses GSTπ, P-gp and LRP. GSTπ expression is positive in 100% well- differentiation ones, so in which way it is correlated with differentiation. But the other two proteins expressions are not related to tumor differentiation and to the degree of tumor invasion. GSTπ may be a new target of chemotherapy in RB. |
| first_indexed | 2024-12-13T16:50:56Z |
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| institution | Directory Open Access Journal |
| issn | 2222-3959 2227-4898 |
| language | English |
| last_indexed | 2024-12-13T16:50:56Z |
| publishDate | 2018-09-01 |
| publisher | Press of International Journal of Ophthalmology (IJO PRESS) |
| record_format | Article |
| series | International Journal of Ophthalmology |
| spelling | doaj.art-6d242a1b21b940ed867fe8657259bc232022-12-21T23:38:03ZengPress of International Journal of Ophthalmology (IJO PRESS)International Journal of Ophthalmology2222-39592227-48982018-09-011191463146610.18240/ijo.2018.09.06Expression of multidrug-resistance associated proteins in human retinoblastoma treated by primary enucleationLi-Juan Tang0Li-Jun Zhou1 Wen-Xin Zhang2 Jian-Yan Lin3 Yong-Ping Li4 Hua-Sheng Yang5Ping Zhang6State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, Guangdong Province, ChinaState Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510061, Guangdong Province, ChinaState Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510062, Guangdong Province, ChinaState Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510063, Guangdong Province, ChinaState Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510064, Guangdong Province, ChinaState Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510065, Guangdong Province, ChinaState Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510066, Guangdong Province, ChinaAIM: To reveal the expression of multidrug-resistance associated proteins: glutathione-S-transferase π (GSTπ), P-glycoprotein (P-gp) and vault protein lung resistance protein (LRP) in retinoblastoma (RB) without any conservative treatment before primary enucleation and to correlate this expression with histopathological tumor features. METHODS: A total of 42 specimens of RB undergone primary enucleation were selected for the research. Sections from the formalin-fixed, paraffin-embedded specimens were stained with HE and immunohistochemistry to detect the expression of GSTπ, P-gp and LRP. RESULTS: GSTπ was expressed in 39/42 (92.86%) RBs and in 9/9 (100%) well-differentiated RBs. P-gp/GSTπ was found in 30 (71.42%) of 42 RBs. Totally 9 (21.43%) tumors were well differentiated and 33 (78.57%) were poorly differentiated. Totally 15 (35.71%) eyes had optic nerve (ON) tumor invasion, 36 (85.71%) had choroidal tumor invasion, and 14 (33.33%) had simultaneous choroidal and ON invasion. There was no statistically significant relationship between P-gp, GSTπ, LRP positivity and the degree of ocular layer tumor invasion and ON tumor invasion (P>0.05). CONCLUSION: RB intrinsically expresses GSTπ, P-gp and LRP. GSTπ expression is positive in 100% well- differentiation ones, so in which way it is correlated with differentiation. But the other two proteins expressions are not related to tumor differentiation and to the degree of tumor invasion. GSTπ may be a new target of chemotherapy in RB.http://www.ijo.cn/en_publish/2018/9/20180906.pdf1466glutathione-S-transferase πP-glycoproteinvault protein lung resistance proteinretinoblastomamultidrug-resistance proteins |
| spellingShingle | Li-Juan Tang Li-Jun Zhou Wen-Xin Zhang Jian-Yan Lin Yong-Ping Li Hua-Sheng Yang Ping Zhang Expression of multidrug-resistance associated proteins in human retinoblastoma treated by primary enucleation International Journal of Ophthalmology 1466 glutathione-S-transferase π P-glycoprotein vault protein lung resistance protein retinoblastoma multidrug-resistance proteins |
| title | Expression of multidrug-resistance associated proteins in human retinoblastoma treated by primary enucleation |
| title_full | Expression of multidrug-resistance associated proteins in human retinoblastoma treated by primary enucleation |
| title_fullStr | Expression of multidrug-resistance associated proteins in human retinoblastoma treated by primary enucleation |
| title_full_unstemmed | Expression of multidrug-resistance associated proteins in human retinoblastoma treated by primary enucleation |
| title_short | Expression of multidrug-resistance associated proteins in human retinoblastoma treated by primary enucleation |
| title_sort | expression of multidrug resistance associated proteins in human retinoblastoma treated by primary enucleation |
| topic | 1466 glutathione-S-transferase π P-glycoprotein vault protein lung resistance protein retinoblastoma multidrug-resistance proteins |
| url | http://www.ijo.cn/en_publish/2018/9/20180906.pdf |
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