Effect of the inoculum dose of three grapevine trunk pathogens on the infection of artificially inoculated pruning wounds
This study assessed the infection rates of different spore inoculum doses of the grapevine trunk pathogens Diplodia seriata, Phaeomoniella chlamydospora and Eutypa lata following artificial inoculation of pruning wounds. Potted vines of cv. Tempranillo were inoculated with doses ranging from 10 to 4...
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| Format: | Article |
| Language: | English |
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Firenze University Press
2015-09-01
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| Series: | Phytopathologia Mediterranea |
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| Online Access: | https://oajournals.fupress.net/index.php/pm/article/view/5625 |
| _version_ | 1818691081471000576 |
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| author | Georgina ELENA Mark SOSNOWSKI Matthew AYRES Pascal LECOMTE Celine BENETREAU Francesc GARCIA-FIGUERES Jordi LUQUE |
| author_facet | Georgina ELENA Mark SOSNOWSKI Matthew AYRES Pascal LECOMTE Celine BENETREAU Francesc GARCIA-FIGUERES Jordi LUQUE |
| author_sort | Georgina ELENA |
| collection | DOAJ |
| description | This study assessed the infection rates of different spore inoculum doses of the grapevine trunk pathogens Diplodia seriata, Phaeomoniella chlamydospora and Eutypa lata following artificial inoculation of pruning wounds. Potted vines of cv. Tempranillo were inoculated with doses ranging from 10 to 4000 conidia per wound of D. seriata and P. chlamydospora and led to recovery percentages of 10–100% for D. seriata and 16–94% for P. chlamydospora. Eutypa lata, when inoculated onto wounds of vines in a mature vineyard (cv. Shiraz) and on detached canes (cv. Cabernet Sauvignon) with a dose range of 10 to 1000 ascospores per wound, led to recovery percentages of 17–95%. In the field assay, there was no difference in recovery from wounds that were exposed to single or double inoculations with the same total spore dose, or between canes that were harvested 7 or 11 months after inoculation. The results obtained in this study showed significant variability in pathogen recovery between trials, comparable with that reported previously, which suggests that factors such as pathogen virulence, environmental parameters and experimental conditions may influence the infection process. According to this study, in order to obtain optimal recovery percentages of 50–70% for robust evaluation of pruning wound treatments, dose ranges of 100-1000 conidia of D. seriata, 100–2000 conidia of P. chlamydospora, and 100–500 ascospores of E. lata per wound would be required. |
| first_indexed | 2024-12-17T12:36:13Z |
| format | Article |
| id | doaj.art-6d28b66c85cc4eaa8a55a0dda2ce5db1 |
| institution | Directory Open Access Journal |
| issn | 0031-9465 1593-2095 |
| language | English |
| last_indexed | 2024-12-17T12:36:13Z |
| publishDate | 2015-09-01 |
| publisher | Firenze University Press |
| record_format | Article |
| series | Phytopathologia Mediterranea |
| spelling | doaj.art-6d28b66c85cc4eaa8a55a0dda2ce5db12022-12-21T21:48:16ZengFirenze University PressPhytopathologia Mediterranea0031-94651593-20952015-09-0154210.14601/Phytopathol_Mediterr-1601014122Effect of the inoculum dose of three grapevine trunk pathogens on the infection of artificially inoculated pruning woundsGeorgina ELENA0Mark SOSNOWSKIMatthew AYRESPascal LECOMTECeline BENETREAUFrancesc GARCIA-FIGUERESJordi LUQUEIRTAThis study assessed the infection rates of different spore inoculum doses of the grapevine trunk pathogens Diplodia seriata, Phaeomoniella chlamydospora and Eutypa lata following artificial inoculation of pruning wounds. Potted vines of cv. Tempranillo were inoculated with doses ranging from 10 to 4000 conidia per wound of D. seriata and P. chlamydospora and led to recovery percentages of 10–100% for D. seriata and 16–94% for P. chlamydospora. Eutypa lata, when inoculated onto wounds of vines in a mature vineyard (cv. Shiraz) and on detached canes (cv. Cabernet Sauvignon) with a dose range of 10 to 1000 ascospores per wound, led to recovery percentages of 17–95%. In the field assay, there was no difference in recovery from wounds that were exposed to single or double inoculations with the same total spore dose, or between canes that were harvested 7 or 11 months after inoculation. The results obtained in this study showed significant variability in pathogen recovery between trials, comparable with that reported previously, which suggests that factors such as pathogen virulence, environmental parameters and experimental conditions may influence the infection process. According to this study, in order to obtain optimal recovery percentages of 50–70% for robust evaluation of pruning wound treatments, dose ranges of 100-1000 conidia of D. seriata, 100–2000 conidia of P. chlamydospora, and 100–500 ascospores of E. lata per wound would be required.https://oajournals.fupress.net/index.php/pm/article/view/5625Diplodia seriataPhaeomoniella chlamydosporaEutypa lataartificial inoculationsinoculum doses |
| spellingShingle | Georgina ELENA Mark SOSNOWSKI Matthew AYRES Pascal LECOMTE Celine BENETREAU Francesc GARCIA-FIGUERES Jordi LUQUE Effect of the inoculum dose of three grapevine trunk pathogens on the infection of artificially inoculated pruning wounds Phytopathologia Mediterranea Diplodia seriata Phaeomoniella chlamydospora Eutypa lata artificial inoculations inoculum doses |
| title | Effect of the inoculum dose of three grapevine trunk pathogens on the infection of artificially inoculated pruning wounds |
| title_full | Effect of the inoculum dose of three grapevine trunk pathogens on the infection of artificially inoculated pruning wounds |
| title_fullStr | Effect of the inoculum dose of three grapevine trunk pathogens on the infection of artificially inoculated pruning wounds |
| title_full_unstemmed | Effect of the inoculum dose of three grapevine trunk pathogens on the infection of artificially inoculated pruning wounds |
| title_short | Effect of the inoculum dose of three grapevine trunk pathogens on the infection of artificially inoculated pruning wounds |
| title_sort | effect of the inoculum dose of three grapevine trunk pathogens on the infection of artificially inoculated pruning wounds |
| topic | Diplodia seriata Phaeomoniella chlamydospora Eutypa lata artificial inoculations inoculum doses |
| url | https://oajournals.fupress.net/index.php/pm/article/view/5625 |
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