Interaction domain of glycoproteins gB and gH of Marek's disease virus and identification of an antiviral peptide with dual functions.
Our previous study reported that both glycoproteins gB and gH of the herpesvirus Marek's disease virus (MDV) contain eleven potential heptad repeat domains. These domains overlap with α-helix-enriched hydrophobic regions, including the gH-derived HR1 (gHH1) and HR3 (gHH3) and gB-derived HR1 (gB...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2013-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3566115?pdf=render |
| _version_ | 1819237032121073664 |
|---|---|
| author | Xiao-Jing Chi Yi-Xin Lu Peng Zhao Chuan-Gen Li Xiao-Jia Wang Ming Wang |
| author_facet | Xiao-Jing Chi Yi-Xin Lu Peng Zhao Chuan-Gen Li Xiao-Jia Wang Ming Wang |
| author_sort | Xiao-Jing Chi |
| collection | DOAJ |
| description | Our previous study reported that both glycoproteins gB and gH of the herpesvirus Marek's disease virus (MDV) contain eleven potential heptad repeat domains. These domains overlap with α-helix-enriched hydrophobic regions, including the gH-derived HR1 (gHH1) and HR3 (gHH3) and gB-derived HR1 (gBH1) regions, which demonstrate effective antiviral activity, with 50% inhibitory concentrations (IC(50)) of less than 12 µM. Plaque formation and chicken embryo infection assays confirmed these results. In this study, biochemical and biophysical analyses detected potential interactions between these peptides. gHH1, gHH3, and gBH1 were found to interact with each other in pairs. The complex formed by gHH3 and gBH1 showed the most stable interaction at a molar ratio of 1:3, the binding between gHH1 and gBH1 was relatively weak, and no interaction was observed between the three HR peptides. These results indicate that gHH3 and gBH1 are likely the key contributors to the interaction between gB and gH. Furthermore, each HR peptide from herpesvirus glycoproteins did not effectively inhibit virus infection compared with peptides from a class I enveloped virus. In this report, the HR mimic peptide modified with a double glutamic acid (EE) or a double lysine (KK) at the non-interactive sites (i.e., solvent-accessible sites) did not noticeably affect the antiviral activity compared with the wild-type HR peptide, whereas tandem peptides from gH-derived gHH1 and gB-derived gBH1 (i.e., gBH1-Linker-gHH1) produced efficient antiviral effects, unlike the individual peptides. The proposed interpretation of inhibition of entry has been addressed. Our results support the hypothesis that the interaction domain between glycoproteins gH and gB is a critical target in the design of inhibitors of herpesvirus infection. |
| first_indexed | 2024-12-23T13:13:52Z |
| format | Article |
| id | doaj.art-6d2ba66ad4f5484e97c9c176dcee1a13 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-23T13:13:52Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-6d2ba66ad4f5484e97c9c176dcee1a132022-12-21T17:45:39ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0182e5476110.1371/journal.pone.0054761Interaction domain of glycoproteins gB and gH of Marek's disease virus and identification of an antiviral peptide with dual functions.Xiao-Jing ChiYi-Xin LuPeng ZhaoChuan-Gen LiXiao-Jia WangMing WangOur previous study reported that both glycoproteins gB and gH of the herpesvirus Marek's disease virus (MDV) contain eleven potential heptad repeat domains. These domains overlap with α-helix-enriched hydrophobic regions, including the gH-derived HR1 (gHH1) and HR3 (gHH3) and gB-derived HR1 (gBH1) regions, which demonstrate effective antiviral activity, with 50% inhibitory concentrations (IC(50)) of less than 12 µM. Plaque formation and chicken embryo infection assays confirmed these results. In this study, biochemical and biophysical analyses detected potential interactions between these peptides. gHH1, gHH3, and gBH1 were found to interact with each other in pairs. The complex formed by gHH3 and gBH1 showed the most stable interaction at a molar ratio of 1:3, the binding between gHH1 and gBH1 was relatively weak, and no interaction was observed between the three HR peptides. These results indicate that gHH3 and gBH1 are likely the key contributors to the interaction between gB and gH. Furthermore, each HR peptide from herpesvirus glycoproteins did not effectively inhibit virus infection compared with peptides from a class I enveloped virus. In this report, the HR mimic peptide modified with a double glutamic acid (EE) or a double lysine (KK) at the non-interactive sites (i.e., solvent-accessible sites) did not noticeably affect the antiviral activity compared with the wild-type HR peptide, whereas tandem peptides from gH-derived gHH1 and gB-derived gBH1 (i.e., gBH1-Linker-gHH1) produced efficient antiviral effects, unlike the individual peptides. The proposed interpretation of inhibition of entry has been addressed. Our results support the hypothesis that the interaction domain between glycoproteins gH and gB is a critical target in the design of inhibitors of herpesvirus infection.http://europepmc.org/articles/PMC3566115?pdf=render |
| spellingShingle | Xiao-Jing Chi Yi-Xin Lu Peng Zhao Chuan-Gen Li Xiao-Jia Wang Ming Wang Interaction domain of glycoproteins gB and gH of Marek's disease virus and identification of an antiviral peptide with dual functions. PLoS ONE |
| title | Interaction domain of glycoproteins gB and gH of Marek's disease virus and identification of an antiviral peptide with dual functions. |
| title_full | Interaction domain of glycoproteins gB and gH of Marek's disease virus and identification of an antiviral peptide with dual functions. |
| title_fullStr | Interaction domain of glycoproteins gB and gH of Marek's disease virus and identification of an antiviral peptide with dual functions. |
| title_full_unstemmed | Interaction domain of glycoproteins gB and gH of Marek's disease virus and identification of an antiviral peptide with dual functions. |
| title_short | Interaction domain of glycoproteins gB and gH of Marek's disease virus and identification of an antiviral peptide with dual functions. |
| title_sort | interaction domain of glycoproteins gb and gh of marek s disease virus and identification of an antiviral peptide with dual functions |
| url | http://europepmc.org/articles/PMC3566115?pdf=render |
| work_keys_str_mv | AT xiaojingchi interactiondomainofglycoproteinsgbandghofmareksdiseasevirusandidentificationofanantiviralpeptidewithdualfunctions AT yixinlu interactiondomainofglycoproteinsgbandghofmareksdiseasevirusandidentificationofanantiviralpeptidewithdualfunctions AT pengzhao interactiondomainofglycoproteinsgbandghofmareksdiseasevirusandidentificationofanantiviralpeptidewithdualfunctions AT chuangenli interactiondomainofglycoproteinsgbandghofmareksdiseasevirusandidentificationofanantiviralpeptidewithdualfunctions AT xiaojiawang interactiondomainofglycoproteinsgbandghofmareksdiseasevirusandidentificationofanantiviralpeptidewithdualfunctions AT mingwang interactiondomainofglycoproteinsgbandghofmareksdiseasevirusandidentificationofanantiviralpeptidewithdualfunctions |