Electrical discharges in water induce spores' DNA damage.
Bacterial spores are one of the most resilient life forms on earth and are involved in many human diseases, such as infectious diarrhea, fatal paralytic illnesses and respiratory infections. Here, we investigated the mechanisms involved in the death of Bacillus pumilus spores after exposure to elect...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2018-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC6089432?pdf=render |
| _version_ | 1818498924030197760 |
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| author | Camille Lamarche Charlotte Da Silva Gauthier Demol Etienne Dague Marie-Pierre Rols Flavien Pillet |
| author_facet | Camille Lamarche Charlotte Da Silva Gauthier Demol Etienne Dague Marie-Pierre Rols Flavien Pillet |
| author_sort | Camille Lamarche |
| collection | DOAJ |
| description | Bacterial spores are one of the most resilient life forms on earth and are involved in many human diseases, such as infectious diarrhea, fatal paralytic illnesses and respiratory infections. Here, we investigated the mechanisms involved in the death of Bacillus pumilus spores after exposure to electric arcs in water. Cutting-edge microscopies at the nanoscale did not reveal any structural disorganization of spores exposed to electric arcs. This result suggested the absence of physical destruction by a propagating shock wave or an exposure to an electric field. However, Pulsed-Field Gel Electrophoresis (PFGE) revealed genomic DNA damage induced by UV radiation and Reactive Oxygen Species (ROS). UV induced single-strand DNA breaks and thymine dimers while ROS were mainly involved in base excision. Our findings revealed a correlation between DNA damage and the treatment of spores with electrical discharges. |
| first_indexed | 2024-12-10T20:22:10Z |
| format | Article |
| id | doaj.art-6d2d7ba3609d4504b8f0f3b45136ba4e |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-10T20:22:10Z |
| publishDate | 2018-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-6d2d7ba3609d4504b8f0f3b45136ba4e2022-12-22T01:34:59ZengPublic Library of Science (PLoS)PLoS ONE1932-62032018-01-01138e020144810.1371/journal.pone.0201448Electrical discharges in water induce spores' DNA damage.Camille LamarcheCharlotte Da SilvaGauthier DemolEtienne DagueMarie-Pierre RolsFlavien PilletBacterial spores are one of the most resilient life forms on earth and are involved in many human diseases, such as infectious diarrhea, fatal paralytic illnesses and respiratory infections. Here, we investigated the mechanisms involved in the death of Bacillus pumilus spores after exposure to electric arcs in water. Cutting-edge microscopies at the nanoscale did not reveal any structural disorganization of spores exposed to electric arcs. This result suggested the absence of physical destruction by a propagating shock wave or an exposure to an electric field. However, Pulsed-Field Gel Electrophoresis (PFGE) revealed genomic DNA damage induced by UV radiation and Reactive Oxygen Species (ROS). UV induced single-strand DNA breaks and thymine dimers while ROS were mainly involved in base excision. Our findings revealed a correlation between DNA damage and the treatment of spores with electrical discharges.http://europepmc.org/articles/PMC6089432?pdf=render |
| spellingShingle | Camille Lamarche Charlotte Da Silva Gauthier Demol Etienne Dague Marie-Pierre Rols Flavien Pillet Electrical discharges in water induce spores' DNA damage. PLoS ONE |
| title | Electrical discharges in water induce spores' DNA damage. |
| title_full | Electrical discharges in water induce spores' DNA damage. |
| title_fullStr | Electrical discharges in water induce spores' DNA damage. |
| title_full_unstemmed | Electrical discharges in water induce spores' DNA damage. |
| title_short | Electrical discharges in water induce spores' DNA damage. |
| title_sort | electrical discharges in water induce spores dna damage |
| url | http://europepmc.org/articles/PMC6089432?pdf=render |
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