Comparing the effect of Hypoalbuminemia on Sodium measured by Indirect versus Direct Ion Selective Electrode Method
Objective: To evaluate the effect of low serum Albumin levels on serum sodium measurement when analyzed by the indirect Ion Selective Electrode (ISE) method and to compare the results with the direct Ion selective electrode (ISE) method. Study Design: Cross-sectional study Place and Duration...
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| Format: | Article |
| Language: | English |
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Army Medical College Rawalpindi
2023-02-01
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| Series: | Pakistan Armed Forces Medical Journal |
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| Online Access: | https://www.pafmj.org/index.php/PAFMJ/article/view/6744 |
| _version_ | 1827976590966915072 |
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| author | Athar Iqbal Paracha Zujaja Hina Haroon Muhammad Aamir Saima Bashir Afshan Bibi Haroon Javaid |
| author_facet | Athar Iqbal Paracha Zujaja Hina Haroon Muhammad Aamir Saima Bashir Afshan Bibi Haroon Javaid |
| author_sort | Athar Iqbal Paracha |
| collection | DOAJ |
| description |
Objective: To evaluate the effect of low serum Albumin levels on serum sodium measurement when analyzed by the indirect Ion Selective Electrode (ISE) method and to compare the results with the direct Ion selective electrode (ISE) method.
Study Design: Cross-sectional study
Place and Duration of Study: Department of Chemical Pathology, Armed Forces Institute of Pathology, Rawalpindi Pakistan, from Jan to Mar 2021.
Methodology: Patients of either gender, aged 18 to 70 years, who were admitted to the Intensive Care Unit of Combined Military Hospital, Rawalpindi, were selected. A total of 200 blood samples were collected in a gel tube. Serum samples were analyzed for albumin and sodium within two hours of sample collection. Sodium levels were measured concurrently by both direct and indirect ISE methods. The difference in results between these two techniques was studied.
Results: Hypoalbuminemia was detected in 176(88%) patients, while 24(12%) had normal albumin levels. In
Hypoalbuminemic patients, serum sodium measurements were higher using the indirect ISE method(134.07±5.55) compared to the direct ISE method (130.95±6.04); the difference between the two techniques was statistically significant (p-value <0.001).Pearson correlation coefficient (r-value = -0.86, p-value <0.001) revealed a symmetrical increase in differences between the two methods as the albumin level decreased.
Conclusion: In Hypoalbuminemic patients, the indirect ISE method gave falsely raised results of serum sodium. In such patients, serum sodium measurement by the Direct ISE method offers more accurate and consistent electrolyte results.
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| first_indexed | 2024-04-09T20:37:57Z |
| format | Article |
| id | doaj.art-6d2de3862b454e768eef1ed1942ad439 |
| institution | Directory Open Access Journal |
| issn | 0030-9648 2411-8842 |
| language | English |
| last_indexed | 2024-04-09T20:37:57Z |
| publishDate | 2023-02-01 |
| publisher | Army Medical College Rawalpindi |
| record_format | Article |
| series | Pakistan Armed Forces Medical Journal |
| spelling | doaj.art-6d2de3862b454e768eef1ed1942ad4392023-03-30T06:25:14ZengArmy Medical College RawalpindiPakistan Armed Forces Medical Journal0030-96482411-88422023-02-0173110.51253/pafmj.v73i1.6744Comparing the effect of Hypoalbuminemia on Sodium measured by Indirect versus Direct Ion Selective Electrode MethodAthar Iqbal Paracha0Zujaja Hina Haroon1Muhammad Aamir2Saima Bashir3Afshan Bibi4Haroon Javaid5Armed Forces Institute of Pathology/National University of Medical Sciences (NUMS) Rawalpindi PakistanArmed Forces Institute of Pathology/National University of Medical Sciences (NUMS) Rawalpindi PakistanArmed Forces Institute of Pathology/National University of Medical Sciences (NUMS) Rawalpindi PakistanArmed Forces Institute of Pathology/National University of Medical Sciences (NUMS) Rawalpindi PakistanArmed Forces Institute of Pathology/National University of Medical Sciences (NUMS) Rawalpindi PakistanHI-TECH Institute of Medical Sciences, Taxila/National University of Medical Sciences (NUMS) Pakistan Objective: To evaluate the effect of low serum Albumin levels on serum sodium measurement when analyzed by the indirect Ion Selective Electrode (ISE) method and to compare the results with the direct Ion selective electrode (ISE) method. Study Design: Cross-sectional study Place and Duration of Study: Department of Chemical Pathology, Armed Forces Institute of Pathology, Rawalpindi Pakistan, from Jan to Mar 2021. Methodology: Patients of either gender, aged 18 to 70 years, who were admitted to the Intensive Care Unit of Combined Military Hospital, Rawalpindi, were selected. A total of 200 blood samples were collected in a gel tube. Serum samples were analyzed for albumin and sodium within two hours of sample collection. Sodium levels were measured concurrently by both direct and indirect ISE methods. The difference in results between these two techniques was studied. Results: Hypoalbuminemia was detected in 176(88%) patients, while 24(12%) had normal albumin levels. In Hypoalbuminemic patients, serum sodium measurements were higher using the indirect ISE method(134.07±5.55) compared to the direct ISE method (130.95±6.04); the difference between the two techniques was statistically significant (p-value <0.001).Pearson correlation coefficient (r-value = -0.86, p-value <0.001) revealed a symmetrical increase in differences between the two methods as the albumin level decreased. Conclusion: In Hypoalbuminemic patients, the indirect ISE method gave falsely raised results of serum sodium. In such patients, serum sodium measurement by the Direct ISE method offers more accurate and consistent electrolyte results. https://www.pafmj.org/index.php/PAFMJ/article/view/6744HypoalbuminemiaIon selective electrode (ISE)Serum sodium measurement |
| spellingShingle | Athar Iqbal Paracha Zujaja Hina Haroon Muhammad Aamir Saima Bashir Afshan Bibi Haroon Javaid Comparing the effect of Hypoalbuminemia on Sodium measured by Indirect versus Direct Ion Selective Electrode Method Pakistan Armed Forces Medical Journal Hypoalbuminemia Ion selective electrode (ISE) Serum sodium measurement |
| title | Comparing the effect of Hypoalbuminemia on Sodium measured by Indirect versus Direct Ion Selective Electrode Method |
| title_full | Comparing the effect of Hypoalbuminemia on Sodium measured by Indirect versus Direct Ion Selective Electrode Method |
| title_fullStr | Comparing the effect of Hypoalbuminemia on Sodium measured by Indirect versus Direct Ion Selective Electrode Method |
| title_full_unstemmed | Comparing the effect of Hypoalbuminemia on Sodium measured by Indirect versus Direct Ion Selective Electrode Method |
| title_short | Comparing the effect of Hypoalbuminemia on Sodium measured by Indirect versus Direct Ion Selective Electrode Method |
| title_sort | comparing the effect of hypoalbuminemia on sodium measured by indirect versus direct ion selective electrode method |
| topic | Hypoalbuminemia Ion selective electrode (ISE) Serum sodium measurement |
| url | https://www.pafmj.org/index.php/PAFMJ/article/view/6744 |
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