microRNA-155, induced by interleukin-1ß, represses the expression of microphthalmia-associated transcription factor (MITF-M) in melanoma cells.
Loss of expression of surface antigens represents a significant problem for cancer immunotherapy. Microphthalmia-associated transcription factor (MITF-M) regulates melanocyte fate by driving expression of many differentiation genes, whose protein products can be recognized by cytolytic T lymphocytes...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2015-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4390329?pdf=render |
| _version_ | 1811331778252636160 |
|---|---|
| author | Nathalie Arts Stefania Cané Marc Hennequart Juliette Lamy Guido Bommer Benoît Van den Eynde Etienne De Plaen |
| author_facet | Nathalie Arts Stefania Cané Marc Hennequart Juliette Lamy Guido Bommer Benoît Van den Eynde Etienne De Plaen |
| author_sort | Nathalie Arts |
| collection | DOAJ |
| description | Loss of expression of surface antigens represents a significant problem for cancer immunotherapy. Microphthalmia-associated transcription factor (MITF-M) regulates melanocyte fate by driving expression of many differentiation genes, whose protein products can be recognized by cytolytic T lymphocytes. We previously reported that interleukin-1ß (IL-1ß) can downregulate MITF-M levels. Here we show that downregulation of MITF-M expression by IL-1ß was paralleled by an upregulation of miR-155 expression in four melanoma lines. We confirmed that miR-155 was able to target endogenous MITF-M in melanoma cells and demonstrated a role for miR-155 in the IL-1ß-induced repression of MITF-M by using an antagomiR. Notably, we also observed a strong negative correlation between MITF-M and miR-155 levels in a mouse model of melanoma. Taken together, our results indicate that MITF-M downregulation by inflammatory stimuli might be partly due to miR-155 upregulation. This could represent a novel mechanism of melanoma immune escape in an inflammatory microenvironment. |
| first_indexed | 2024-04-13T16:25:48Z |
| format | Article |
| id | doaj.art-6d344dfb97f2435a8e08e4895c2687fc |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-13T16:25:48Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-6d344dfb97f2435a8e08e4895c2687fc2022-12-22T02:39:45ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01104e012251710.1371/journal.pone.0122517microRNA-155, induced by interleukin-1ß, represses the expression of microphthalmia-associated transcription factor (MITF-M) in melanoma cells.Nathalie ArtsStefania CanéMarc HennequartJuliette LamyGuido BommerBenoît Van den EyndeEtienne De PlaenLoss of expression of surface antigens represents a significant problem for cancer immunotherapy. Microphthalmia-associated transcription factor (MITF-M) regulates melanocyte fate by driving expression of many differentiation genes, whose protein products can be recognized by cytolytic T lymphocytes. We previously reported that interleukin-1ß (IL-1ß) can downregulate MITF-M levels. Here we show that downregulation of MITF-M expression by IL-1ß was paralleled by an upregulation of miR-155 expression in four melanoma lines. We confirmed that miR-155 was able to target endogenous MITF-M in melanoma cells and demonstrated a role for miR-155 in the IL-1ß-induced repression of MITF-M by using an antagomiR. Notably, we also observed a strong negative correlation between MITF-M and miR-155 levels in a mouse model of melanoma. Taken together, our results indicate that MITF-M downregulation by inflammatory stimuli might be partly due to miR-155 upregulation. This could represent a novel mechanism of melanoma immune escape in an inflammatory microenvironment.http://europepmc.org/articles/PMC4390329?pdf=render |
| spellingShingle | Nathalie Arts Stefania Cané Marc Hennequart Juliette Lamy Guido Bommer Benoît Van den Eynde Etienne De Plaen microRNA-155, induced by interleukin-1ß, represses the expression of microphthalmia-associated transcription factor (MITF-M) in melanoma cells. PLoS ONE |
| title | microRNA-155, induced by interleukin-1ß, represses the expression of microphthalmia-associated transcription factor (MITF-M) in melanoma cells. |
| title_full | microRNA-155, induced by interleukin-1ß, represses the expression of microphthalmia-associated transcription factor (MITF-M) in melanoma cells. |
| title_fullStr | microRNA-155, induced by interleukin-1ß, represses the expression of microphthalmia-associated transcription factor (MITF-M) in melanoma cells. |
| title_full_unstemmed | microRNA-155, induced by interleukin-1ß, represses the expression of microphthalmia-associated transcription factor (MITF-M) in melanoma cells. |
| title_short | microRNA-155, induced by interleukin-1ß, represses the expression of microphthalmia-associated transcription factor (MITF-M) in melanoma cells. |
| title_sort | microrna 155 induced by interleukin 1ss represses the expression of microphthalmia associated transcription factor mitf m in melanoma cells |
| url | http://europepmc.org/articles/PMC4390329?pdf=render |
| work_keys_str_mv | AT nathaliearts microrna155inducedbyinterleukin1ßrepressestheexpressionofmicrophthalmiaassociatedtranscriptionfactormitfminmelanomacells AT stefaniacane microrna155inducedbyinterleukin1ßrepressestheexpressionofmicrophthalmiaassociatedtranscriptionfactormitfminmelanomacells AT marchennequart microrna155inducedbyinterleukin1ßrepressestheexpressionofmicrophthalmiaassociatedtranscriptionfactormitfminmelanomacells AT juliettelamy microrna155inducedbyinterleukin1ßrepressestheexpressionofmicrophthalmiaassociatedtranscriptionfactormitfminmelanomacells AT guidobommer microrna155inducedbyinterleukin1ßrepressestheexpressionofmicrophthalmiaassociatedtranscriptionfactormitfminmelanomacells AT benoitvandeneynde microrna155inducedbyinterleukin1ßrepressestheexpressionofmicrophthalmiaassociatedtranscriptionfactormitfminmelanomacells AT etiennedeplaen microrna155inducedbyinterleukin1ßrepressestheexpressionofmicrophthalmiaassociatedtranscriptionfactormitfminmelanomacells |