Preparation and Evaluation of Antioxidant Activity of Haematococcus pluvialis Hydrolysate
In order to make high value use of the algal residue, this paper presented a study on the preparation of antioxidant enzymatic hydrolysate from Haematococcus pluvialis residue. Single factor investigation and the response surface methodology (RSM) were used to optimize the hydrolysis process of H. p...
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| Format: | Article |
| Language: | zho |
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The editorial department of Science and Technology of Food Industry
2022-09-01
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| Series: | Shipin gongye ke-ji |
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| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021120047 |
| _version_ | 1811307636981760000 |
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| author | Wanshi HE Jin XU Yong CAO Zhongming WANG Xiaojuan LIU |
| author_facet | Wanshi HE Jin XU Yong CAO Zhongming WANG Xiaojuan LIU |
| author_sort | Wanshi HE |
| collection | DOAJ |
| description | In order to make high value use of the algal residue, this paper presented a study on the preparation of antioxidant enzymatic hydrolysate from Haematococcus pluvialis residue. Single factor investigation and the response surface methodology (RSM) were used to optimize the hydrolysis process of H. pluvialis residue, including substrate concentration, dosage of proteases, dosage of proteases, temperature, pH and time. The resulting hydrolysates were comparatively evaluated for their ABTS scavenging capacity and degree of hydrolysis. Subsequently, the antioxidant ability and amino acid composition of the optimal hydrolysate were investigated. The results showed that under the optimal hydrolysis conditions: Substrate concentration of 9% (w:v), dosage of proteases of 0.7% (w:w), temperature of 40 ℃, pH11.5 and time of 3 h, the highest ABTS scavenging activity with IC50 of 481.574 μg/mL and FRAP value of 19.641 μmol FeSO4·7H2O /g (concentration of 1000 μg/mL) were obtained. Antioxidant enzymatic hydrolysate with 34.4% essential amino acid not only contained high nutritional value but also involved many hydrophobic amino acids closely related to antioxidant activity. This research provides a reference for high value-added applications of H. pluvialis. |
| first_indexed | 2024-04-13T09:07:26Z |
| format | Article |
| id | doaj.art-6d3463c31059413f93521d66ac1b77f8 |
| institution | Directory Open Access Journal |
| issn | 1002-0306 |
| language | zho |
| last_indexed | 2024-04-13T09:07:26Z |
| publishDate | 2022-09-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
| record_format | Article |
| series | Shipin gongye ke-ji |
| spelling | doaj.art-6d3463c31059413f93521d66ac1b77f82022-12-22T02:52:57ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062022-09-01431724825710.13386/j.issn1002-0306.20211200472021120047-17Preparation and Evaluation of Antioxidant Activity of Haematococcus pluvialis HydrolysateWanshi HE0Jin XU1Yong CAO2Zhongming WANG3Xiaojuan LIU4Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangdong Provincial Key Laboratory of New and Renewable Energy Research and Development, Guangzhou 510640, ChinaGuangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangdong Provincial Key Laboratory of New and Renewable Energy Research and Development, Guangzhou 510640, ChinaCollege of Food Science, South China Agricultural University, Guangdong Provincial Key Laboratory of Nutraceuticals and Functional Foods, Guangzhou 510642, ChinaGuangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangdong Provincial Key Laboratory of New and Renewable Energy Research and Development, Guangzhou 510640, ChinaCollege of Food Science, South China Agricultural University, Guangdong Provincial Key Laboratory of Nutraceuticals and Functional Foods, Guangzhou 510642, ChinaIn order to make high value use of the algal residue, this paper presented a study on the preparation of antioxidant enzymatic hydrolysate from Haematococcus pluvialis residue. Single factor investigation and the response surface methodology (RSM) were used to optimize the hydrolysis process of H. pluvialis residue, including substrate concentration, dosage of proteases, dosage of proteases, temperature, pH and time. The resulting hydrolysates were comparatively evaluated for their ABTS scavenging capacity and degree of hydrolysis. Subsequently, the antioxidant ability and amino acid composition of the optimal hydrolysate were investigated. The results showed that under the optimal hydrolysis conditions: Substrate concentration of 9% (w:v), dosage of proteases of 0.7% (w:w), temperature of 40 ℃, pH11.5 and time of 3 h, the highest ABTS scavenging activity with IC50 of 481.574 μg/mL and FRAP value of 19.641 μmol FeSO4·7H2O /g (concentration of 1000 μg/mL) were obtained. Antioxidant enzymatic hydrolysate with 34.4% essential amino acid not only contained high nutritional value but also involved many hydrophobic amino acids closely related to antioxidant activity. This research provides a reference for high value-added applications of H. pluvialis.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021120047haematococcus pluvialisenzymatic hydrolysatesantioxidant abilityresponse surface methodology (rsm)amino acid composition |
| spellingShingle | Wanshi HE Jin XU Yong CAO Zhongming WANG Xiaojuan LIU Preparation and Evaluation of Antioxidant Activity of Haematococcus pluvialis Hydrolysate Shipin gongye ke-ji haematococcus pluvialis enzymatic hydrolysates antioxidant ability response surface methodology (rsm) amino acid composition |
| title | Preparation and Evaluation of Antioxidant Activity of Haematococcus pluvialis Hydrolysate |
| title_full | Preparation and Evaluation of Antioxidant Activity of Haematococcus pluvialis Hydrolysate |
| title_fullStr | Preparation and Evaluation of Antioxidant Activity of Haematococcus pluvialis Hydrolysate |
| title_full_unstemmed | Preparation and Evaluation of Antioxidant Activity of Haematococcus pluvialis Hydrolysate |
| title_short | Preparation and Evaluation of Antioxidant Activity of Haematococcus pluvialis Hydrolysate |
| title_sort | preparation and evaluation of antioxidant activity of haematococcus pluvialis hydrolysate |
| topic | haematococcus pluvialis enzymatic hydrolysates antioxidant ability response surface methodology (rsm) amino acid composition |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021120047 |
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