Geographical distribution of Trypanosoma cruzi in triatomine vectors in the State of Mato Grosso do Sul, Brazil
Introduction This work presents the initial findings of a molecular epidemiological investigation of Trypanosoma cruzi in triatomine insects in State of Mato Grosso do Sul. Metho...
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Sociedade Brasileira de Medicina Tropical (SBMT)
2014-12-01
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| Series: | Revista da Sociedade Brasileira de Medicina Tropical |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822014000600747&lng=en&tlng=en |
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| author | Marlon Cezar Cominetti Bárbara Guimarães Csordas Rodrigo Casquero Cunha Renato Andreotti |
| author_facet | Marlon Cezar Cominetti Bárbara Guimarães Csordas Rodrigo Casquero Cunha Renato Andreotti |
| author_sort | Marlon Cezar Cominetti |
| collection | DOAJ |
| description | Introduction This work presents the initial findings of a molecular epidemiological investigation of Trypanosoma cruzi in triatomine insects in State of Mato Grosso do Sul. Methods A total of 511 triatomines from different regions of the state were examined. Deoxyribonucleic acid (DNA) was extracted from the intestinal contents of the insects using phenol-chloroform-isoamyl alcohol (25:24:1). Polymerase chain reaction (PCR) using primers 121/122 targeting DNA kinetoplast (kDNA) was then performed to identify T. cruzi, and positive samples were subjected to PCR using the primer pair TcSC5D-F/R followed by restriction fragment length polymorphism (RFLP) with the restriction enzymes SphI and HpaI (1 U/reaction), cloning and sequencing. Results One hundred samples were positive for T. cruzi, and three discrete typing units (DTUs) were identified (TcI, TcII, and TcBat). Triatoma sordida had the highest T. cruzi occurrence (83.3%), and DTUs were found in three samples: 58.3% of the samples were TcI, 33.3% were TcII and 8.3% were TcBat. There was a clear geographical distribution of the DTUs throughout the state, with TcI, TcII and TcBat located in the center, TcI located in the east, and TcII located in the west. Conclusions This study showed the occurrence of overlapping DTUs in State of Mato Grosso do Sul. The distributions of the DTUs were different, with TcI, TcII and TcBat in the center of the state, TcI predominantly in the east, and TcII in the west. Further studies may reveal a more defined mosaic distribution of DTUs in MS. |
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| format | Article |
| id | doaj.art-6d350f4d24fd41a880334ca8c02a91e4 |
| institution | Directory Open Access Journal |
| issn | 1678-9849 |
| language | English |
| last_indexed | 2024-12-12T09:38:38Z |
| publishDate | 2014-12-01 |
| publisher | Sociedade Brasileira de Medicina Tropical (SBMT) |
| record_format | Article |
| series | Revista da Sociedade Brasileira de Medicina Tropical |
| spelling | doaj.art-6d350f4d24fd41a880334ca8c02a91e42022-12-22T00:28:39ZengSociedade Brasileira de Medicina Tropical (SBMT)Revista da Sociedade Brasileira de Medicina Tropical1678-98492014-12-0147674775510.1590/0037-8682-0234-2014S0037-86822014000600747Geographical distribution of Trypanosoma cruzi in triatomine vectors in the State of Mato Grosso do Sul, BrazilMarlon Cezar CominettiBárbara Guimarães CsordasRodrigo Casquero CunhaRenato AndreottiIntroduction This work presents the initial findings of a molecular epidemiological investigation of Trypanosoma cruzi in triatomine insects in State of Mato Grosso do Sul. Methods A total of 511 triatomines from different regions of the state were examined. Deoxyribonucleic acid (DNA) was extracted from the intestinal contents of the insects using phenol-chloroform-isoamyl alcohol (25:24:1). Polymerase chain reaction (PCR) using primers 121/122 targeting DNA kinetoplast (kDNA) was then performed to identify T. cruzi, and positive samples were subjected to PCR using the primer pair TcSC5D-F/R followed by restriction fragment length polymorphism (RFLP) with the restriction enzymes SphI and HpaI (1 U/reaction), cloning and sequencing. Results One hundred samples were positive for T. cruzi, and three discrete typing units (DTUs) were identified (TcI, TcII, and TcBat). Triatoma sordida had the highest T. cruzi occurrence (83.3%), and DTUs were found in three samples: 58.3% of the samples were TcI, 33.3% were TcII and 8.3% were TcBat. There was a clear geographical distribution of the DTUs throughout the state, with TcI, TcII and TcBat located in the center, TcI located in the east, and TcII located in the west. Conclusions This study showed the occurrence of overlapping DTUs in State of Mato Grosso do Sul. The distributions of the DTUs were different, with TcI, TcII and TcBat in the center of the state, TcI predominantly in the east, and TcII in the west. Further studies may reveal a more defined mosaic distribution of DTUs in MS.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822014000600747&lng=en&tlng=enTrypanosoma cruzi DTUsPCRRestriction fragment length polymorphismMicroscopy examination |
| spellingShingle | Marlon Cezar Cominetti Bárbara Guimarães Csordas Rodrigo Casquero Cunha Renato Andreotti Geographical distribution of Trypanosoma cruzi in triatomine vectors in the State of Mato Grosso do Sul, Brazil Revista da Sociedade Brasileira de Medicina Tropical Trypanosoma cruzi DTUs PCR Restriction fragment length polymorphism Microscopy examination |
| title | Geographical distribution of Trypanosoma cruzi in triatomine vectors in the State of Mato Grosso do Sul, Brazil |
| title_full | Geographical distribution of Trypanosoma cruzi in triatomine vectors in the State of Mato Grosso do Sul, Brazil |
| title_fullStr | Geographical distribution of Trypanosoma cruzi in triatomine vectors in the State of Mato Grosso do Sul, Brazil |
| title_full_unstemmed | Geographical distribution of Trypanosoma cruzi in triatomine vectors in the State of Mato Grosso do Sul, Brazil |
| title_short | Geographical distribution of Trypanosoma cruzi in triatomine vectors in the State of Mato Grosso do Sul, Brazil |
| title_sort | geographical distribution of trypanosoma cruzi in triatomine vectors in the state of mato grosso do sul brazil |
| topic | Trypanosoma cruzi DTUs PCR Restriction fragment length polymorphism Microscopy examination |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822014000600747&lng=en&tlng=en |
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