Respiratory virus detection in the upper respiratory tract of asymptomatic, community-dwelling older people
Abstract Background The prevalence of virus positivity in the upper respiratory tract of asymptomatic community-dwelling older people remains elusive. Our objective was to investigate the prevalence of respiratory virus PCR positivity in asymptomatic community-dwelling older people using saliva samp...
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| Format: | Article |
| Language: | English |
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BMC
2022-04-01
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| Series: | BMC Infectious Diseases |
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| Online Access: | https://doi.org/10.1186/s12879-022-07355-w |
| _version_ | 1811289549767180288 |
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| author | Ikkoh Yasuda Motoi Suzuki Haruka Maeda Mayumi Terada Eiichiro Sando Chris Fook Sheng Ng Hirono Otomaru Lay-Myint Yoshida Konosuke Morimoto |
| author_facet | Ikkoh Yasuda Motoi Suzuki Haruka Maeda Mayumi Terada Eiichiro Sando Chris Fook Sheng Ng Hirono Otomaru Lay-Myint Yoshida Konosuke Morimoto |
| author_sort | Ikkoh Yasuda |
| collection | DOAJ |
| description | Abstract Background The prevalence of virus positivity in the upper respiratory tract of asymptomatic community-dwelling older people remains elusive. Our objective was to investigate the prevalence of respiratory virus PCR positivity in asymptomatic community-dwelling older people using saliva samples and nasopharyngeal and oropharyngeal swabs. Methods We analyzed 504 community-dwelling adults aged ≥ 65 years who were ambulatory and enrolled in a cross-sectional study conducted from February to December 2018 in Nagasaki city, Japan. Fourteen respiratory viruses were identified in saliva, nasopharyngeal and oropharyngeal samples using multiplex PCR assays. Results The prevalences of PCR positivity for rhinovirus, influenza A, enterovirus and any respiratory virus were 12.9% (95% CI: 10.1–16.1%), 7.1% (95% CI: 5.1–9.8%), 6.9% (95% CI: 4.9–9.5%) and 25.2% (95% CI: 21.5–29.2%), respectively. Rhinovirus was detected in 21.5% of subjects, influenza A in 38.9% of subjects, enterovirus in 51.4% of subjects and any virus in 32.3% of subjects using only saliva sampling. Conclusions The prevalences of several respiratory viruses were higher than the percentages reported previously in pharyngeal samples from younger adults. Saliva sampling is a potentially useful method for respiratory virus detection in asymptomatic populations. |
| first_indexed | 2024-04-13T03:56:46Z |
| format | Article |
| id | doaj.art-6d37a7093827453c8cd15d229e9f52e8 |
| institution | Directory Open Access Journal |
| issn | 1471-2334 |
| language | English |
| last_indexed | 2024-04-13T03:56:46Z |
| publishDate | 2022-04-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Infectious Diseases |
| spelling | doaj.art-6d37a7093827453c8cd15d229e9f52e82022-12-22T03:03:36ZengBMCBMC Infectious Diseases1471-23342022-04-012211710.1186/s12879-022-07355-wRespiratory virus detection in the upper respiratory tract of asymptomatic, community-dwelling older peopleIkkoh Yasuda0Motoi Suzuki1Haruka Maeda2Mayumi Terada3Eiichiro Sando4Chris Fook Sheng Ng5Hirono Otomaru6Lay-Myint Yoshida7Konosuke Morimoto8Department of Clinical Medicine, Institute of Tropical Medicine, Nagasaki UniversityDepartment of Clinical Medicine, Institute of Tropical Medicine, Nagasaki UniversityDepartment of Clinical Medicine, Institute of Tropical Medicine, Nagasaki UniversityDepartment of Clinical Medicine, Institute of Tropical Medicine, Nagasaki UniversityDepartment of Clinical Medicine, Institute of Tropical Medicine, Nagasaki UniversityDepartment of Global Health Policy, Graduate School of Medicine, The University of TokyoDepartment of Pediatric Infectious Diseases, Institute of Tropical Medicine, Nagasaki UniversityDepartment of Pediatric Infectious Diseases, Institute of Tropical Medicine, Nagasaki UniversityDepartment of Clinical Medicine, Institute of Tropical Medicine, Nagasaki UniversityAbstract Background The prevalence of virus positivity in the upper respiratory tract of asymptomatic community-dwelling older people remains elusive. Our objective was to investigate the prevalence of respiratory virus PCR positivity in asymptomatic community-dwelling older people using saliva samples and nasopharyngeal and oropharyngeal swabs. Methods We analyzed 504 community-dwelling adults aged ≥ 65 years who were ambulatory and enrolled in a cross-sectional study conducted from February to December 2018 in Nagasaki city, Japan. Fourteen respiratory viruses were identified in saliva, nasopharyngeal and oropharyngeal samples using multiplex PCR assays. Results The prevalences of PCR positivity for rhinovirus, influenza A, enterovirus and any respiratory virus were 12.9% (95% CI: 10.1–16.1%), 7.1% (95% CI: 5.1–9.8%), 6.9% (95% CI: 4.9–9.5%) and 25.2% (95% CI: 21.5–29.2%), respectively. Rhinovirus was detected in 21.5% of subjects, influenza A in 38.9% of subjects, enterovirus in 51.4% of subjects and any virus in 32.3% of subjects using only saliva sampling. Conclusions The prevalences of several respiratory viruses were higher than the percentages reported previously in pharyngeal samples from younger adults. Saliva sampling is a potentially useful method for respiratory virus detection in asymptomatic populations.https://doi.org/10.1186/s12879-022-07355-wPrevalenceRespiratory virusPolymerase chain reactionAsymptomaticCommunity-dwellingOlder people |
| spellingShingle | Ikkoh Yasuda Motoi Suzuki Haruka Maeda Mayumi Terada Eiichiro Sando Chris Fook Sheng Ng Hirono Otomaru Lay-Myint Yoshida Konosuke Morimoto Respiratory virus detection in the upper respiratory tract of asymptomatic, community-dwelling older people BMC Infectious Diseases Prevalence Respiratory virus Polymerase chain reaction Asymptomatic Community-dwelling Older people |
| title | Respiratory virus detection in the upper respiratory tract of asymptomatic, community-dwelling older people |
| title_full | Respiratory virus detection in the upper respiratory tract of asymptomatic, community-dwelling older people |
| title_fullStr | Respiratory virus detection in the upper respiratory tract of asymptomatic, community-dwelling older people |
| title_full_unstemmed | Respiratory virus detection in the upper respiratory tract of asymptomatic, community-dwelling older people |
| title_short | Respiratory virus detection in the upper respiratory tract of asymptomatic, community-dwelling older people |
| title_sort | respiratory virus detection in the upper respiratory tract of asymptomatic community dwelling older people |
| topic | Prevalence Respiratory virus Polymerase chain reaction Asymptomatic Community-dwelling Older people |
| url | https://doi.org/10.1186/s12879-022-07355-w |
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