Omics Sequencing of <i>Saccharomyces cerevisiae</i> Strain with Improved Capacity for Ethanol Production
<i>Saccharomyces cerevisiae</i> is the most important industrial microorganism used to fuel ethanol production worldwide. Herein, we obtained a mutant <i>S. cerevisiae</i> strain with improved capacity for ethanol fermentation, from 13.72% (<i>v</i>/<i>v<...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-05-01
|
| Series: | Fermentation |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2311-5637/9/5/483 |
| _version_ | 1797600162956181504 |
|---|---|
| author | Zhilong Lu Ling Guo Xiaoling Chen Qi Lu Yanling Wu Dong Chen Renzhi Wu Ying Chen |
| author_facet | Zhilong Lu Ling Guo Xiaoling Chen Qi Lu Yanling Wu Dong Chen Renzhi Wu Ying Chen |
| author_sort | Zhilong Lu |
| collection | DOAJ |
| description | <i>Saccharomyces cerevisiae</i> is the most important industrial microorganism used to fuel ethanol production worldwide. Herein, we obtained a mutant <i>S. cerevisiae</i> strain with improved capacity for ethanol fermentation, from 13.72% (<i>v</i>/<i>v</i> for the wild-type strain) to 16.13% (<i>v</i>/<i>v</i> for the mutant strain), and analyzed its genomic structure and gene expression changes. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment revealed that the changed genes were mainly enriched in the pathways of carbohydrate metabolism, amino acid metabolism, metabolism of cofactors and vitamins, and lipid metabolism. The gene expression trends of the two strains were recorded during fermentation to create a timeline. Venn diagram analysis revealed exclusive genes in the mutant strain. KEGG enrichment of these genes showed upregulation of genes involved in sugar metabolism, mitogen-activated protein kinase pathway, fatty acid and amino acid degradation, and downregulation of genes involved in oxidative phosphorylation, ribosome, fatty acid and amino acid biogenesis. Protein interaction analysis of these genes showed that glucose-6-phosphate isomerase 1, signal peptidase complex subunit 3, 6-phosphofructokinase 2, and trifunctional aldehyde reductase were the major hub genes in the network, linking pathways together. These findings provide new insights into the adaptive metabolism of <i>S. cerevisiae</i> for ethanol production and a framework for the construction of engineered strains of <i>S. cerevisiae</i> with excellent ethanol fermentation capacity. |
| first_indexed | 2024-03-11T03:44:41Z |
| format | Article |
| id | doaj.art-6d38d0c528404314942423a9b1f5d7d3 |
| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-03-11T03:44:41Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj.art-6d38d0c528404314942423a9b1f5d7d32023-11-18T01:18:29ZengMDPI AGFermentation2311-56372023-05-019548310.3390/fermentation9050483Omics Sequencing of <i>Saccharomyces cerevisiae</i> Strain with Improved Capacity for Ethanol ProductionZhilong Lu0Ling Guo1Xiaoling Chen2Qi Lu3Yanling Wu4Dong Chen5Renzhi Wu6Ying Chen7National Key Laboratory of Non-food Biomass Technology, Guangxi Academy of Sciences, Nanning 530007, ChinaCAS Key Laboratory of Environmental and Applied Microbiology, Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, ChinaNational Key Laboratory of Non-food Biomass Technology, Guangxi Academy of Sciences, Nanning 530007, ChinaNational Key Laboratory of Non-food Biomass Technology, Guangxi Academy of Sciences, Nanning 530007, ChinaNational Key Laboratory of Non-food Biomass Technology, Guangxi Academy of Sciences, Nanning 530007, ChinaNational Key Laboratory of Non-food Biomass Technology, Guangxi Academy of Sciences, Nanning 530007, ChinaNational Key Laboratory of Non-food Biomass Technology, Guangxi Academy of Sciences, Nanning 530007, ChinaNational Key Laboratory of Non-food Biomass Technology, Guangxi Academy of Sciences, Nanning 530007, China<i>Saccharomyces cerevisiae</i> is the most important industrial microorganism used to fuel ethanol production worldwide. Herein, we obtained a mutant <i>S. cerevisiae</i> strain with improved capacity for ethanol fermentation, from 13.72% (<i>v</i>/<i>v</i> for the wild-type strain) to 16.13% (<i>v</i>/<i>v</i> for the mutant strain), and analyzed its genomic structure and gene expression changes. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment revealed that the changed genes were mainly enriched in the pathways of carbohydrate metabolism, amino acid metabolism, metabolism of cofactors and vitamins, and lipid metabolism. The gene expression trends of the two strains were recorded during fermentation to create a timeline. Venn diagram analysis revealed exclusive genes in the mutant strain. KEGG enrichment of these genes showed upregulation of genes involved in sugar metabolism, mitogen-activated protein kinase pathway, fatty acid and amino acid degradation, and downregulation of genes involved in oxidative phosphorylation, ribosome, fatty acid and amino acid biogenesis. Protein interaction analysis of these genes showed that glucose-6-phosphate isomerase 1, signal peptidase complex subunit 3, 6-phosphofructokinase 2, and trifunctional aldehyde reductase were the major hub genes in the network, linking pathways together. These findings provide new insights into the adaptive metabolism of <i>S. cerevisiae</i> for ethanol production and a framework for the construction of engineered strains of <i>S. cerevisiae</i> with excellent ethanol fermentation capacity.https://www.mdpi.com/2311-5637/9/5/483<i>Saccharomyces cerevisiae</i>ethanol fermentationomics sequencingexpression trendprotein interactionhub genes |
| spellingShingle | Zhilong Lu Ling Guo Xiaoling Chen Qi Lu Yanling Wu Dong Chen Renzhi Wu Ying Chen Omics Sequencing of <i>Saccharomyces cerevisiae</i> Strain with Improved Capacity for Ethanol Production Fermentation <i>Saccharomyces cerevisiae</i> ethanol fermentation omics sequencing expression trend protein interaction hub genes |
| title | Omics Sequencing of <i>Saccharomyces cerevisiae</i> Strain with Improved Capacity for Ethanol Production |
| title_full | Omics Sequencing of <i>Saccharomyces cerevisiae</i> Strain with Improved Capacity for Ethanol Production |
| title_fullStr | Omics Sequencing of <i>Saccharomyces cerevisiae</i> Strain with Improved Capacity for Ethanol Production |
| title_full_unstemmed | Omics Sequencing of <i>Saccharomyces cerevisiae</i> Strain with Improved Capacity for Ethanol Production |
| title_short | Omics Sequencing of <i>Saccharomyces cerevisiae</i> Strain with Improved Capacity for Ethanol Production |
| title_sort | omics sequencing of i saccharomyces cerevisiae i strain with improved capacity for ethanol production |
| topic | <i>Saccharomyces cerevisiae</i> ethanol fermentation omics sequencing expression trend protein interaction hub genes |
| url | https://www.mdpi.com/2311-5637/9/5/483 |
| work_keys_str_mv | AT zhilonglu omicssequencingofisaccharomycescerevisiaeistrainwithimprovedcapacityforethanolproduction AT lingguo omicssequencingofisaccharomycescerevisiaeistrainwithimprovedcapacityforethanolproduction AT xiaolingchen omicssequencingofisaccharomycescerevisiaeistrainwithimprovedcapacityforethanolproduction AT qilu omicssequencingofisaccharomycescerevisiaeistrainwithimprovedcapacityforethanolproduction AT yanlingwu omicssequencingofisaccharomycescerevisiaeistrainwithimprovedcapacityforethanolproduction AT dongchen omicssequencingofisaccharomycescerevisiaeistrainwithimprovedcapacityforethanolproduction AT renzhiwu omicssequencingofisaccharomycescerevisiaeistrainwithimprovedcapacityforethanolproduction AT yingchen omicssequencingofisaccharomycescerevisiaeistrainwithimprovedcapacityforethanolproduction |