Borneo herbal plant extracts as a natural medication for prophylaxis and treatment of Aeromonas hydrophila and Pseudomonas fluorescens infection in tilapia (Oreochromis niloticus) [version 2; peer review: 2 approved, 1 approved with reservations]
Background: The combination of some plant extracts to prevent and treat bacterial infections is gaining momentum, because of effectiveness against certain bacteria. This study aims to describe the antibacterial and immunostimulant abilities of Boesenbergia pandurata (BP), Solanum ferox (SF) and Zing...
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| Format: | Article |
| Language: | English |
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F1000 Research Ltd
2019-02-01
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| Series: | F1000Research |
| Online Access: | https://f1000research.com/articles/7-1847/v2 |
| _version_ | 1818842339963043840 |
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| author | Esti Handayani Hardi Rudy Agung Nugroho Irawan Wijaya Kusuma Wiwin Suwinarti Agung Sudaryono Rita Rostika |
| author_facet | Esti Handayani Hardi Rudy Agung Nugroho Irawan Wijaya Kusuma Wiwin Suwinarti Agung Sudaryono Rita Rostika |
| author_sort | Esti Handayani Hardi |
| collection | DOAJ |
| description | Background: The combination of some plant extracts to prevent and treat bacterial infections is gaining momentum, because of effectiveness against certain bacteria. This study aims to describe the antibacterial and immunostimulant abilities of Boesenbergia pandurata (BP), Solanum ferox (SF) and Zingiber Zerumbet (ZZ) plant extracts to treat and prevent Aeromonas hydrophila and Pseudomonas fluorescens infection on Tilapia (Oreochromis niloticus). Methods: Tilapia (initial weight 15±2 g) were injected intramuscularly (0.1 ml/fish) with a combination of A. hydrophila and P. fluorescens at a density of 1×10 5 CFU ml -1 of each bacteria. Treatment trials were performed at day 7 post-injection with each combined extract, while the prevention trial was performed by including the combined extract into the commercial diet for six and seven days prior to injection. Various extract combinations were 60 mg SF extract/kg feed with 40 mg ZZ/kg feed (SF60/ZZ40), SF50/ZZ50, BP90/SF10, and BP50/SF50. Haemato-immunological parameters were performed for four weeks. Results: In prevention trials, tilapia fed SF50/ZZ50 showed a significant increase of white and red blood cells. Similarly, significantly increased haematocrit was found in tilapia fed SF50/ZZ50 in the treatment trial but not in the prevention trial. In both trials, haemoglobin of tilapia was not affected by any combined extracts but decreased the number of bacteria. Phagocytic index, respiratory burst, lysozyme activity and survival rate of fish fed combined extracts were found significantly higher than controls. The amount of pathogenic bacteria in fish fed combined extracts was lower than the control at week 4 (P<0.05). In both trials The percentage of survival rate and relative percent survival of tilapia fed SF 50/ZZ 50, showed the optimum results compared to the other combinations. Conclusions: The combined extract in feed, especially SF50/ZZ50 has a positive effect on the tilapia's innate immune system of tilapia to treat and prevent bacterial infections. |
| first_indexed | 2024-12-19T04:40:25Z |
| format | Article |
| id | doaj.art-6d3f3af8584045f8a42b033c61d379b8 |
| institution | Directory Open Access Journal |
| issn | 2046-1402 |
| language | English |
| last_indexed | 2024-12-19T04:40:25Z |
| publishDate | 2019-02-01 |
| publisher | F1000 Research Ltd |
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| spelling | doaj.art-6d3f3af8584045f8a42b033c61d379b82022-12-21T20:35:38ZengF1000 Research LtdF1000Research2046-14022019-02-01710.12688/f1000research.16902.219635Borneo herbal plant extracts as a natural medication for prophylaxis and treatment of Aeromonas hydrophila and Pseudomonas fluorescens infection in tilapia (Oreochromis niloticus) [version 2; peer review: 2 approved, 1 approved with reservations]Esti Handayani Hardi0Rudy Agung Nugroho1Irawan Wijaya Kusuma2Wiwin Suwinarti3Agung Sudaryono4Rita Rostika5Microbiology Laboratory, Department of Aquaculture, Faculty of Fisheries and Marine Science, Mulawarman University, Samarinda, East Kalimantan, 75123, IndonesiaAnimal Physiology, Development and Molecular Laboratory, Department of Biology, Faculty of Mathematics and Natural Sciences, Mulawarman University, Samarinda, East Kalimantan, 75123, IndonesiaLaboratory of Forest Product Chemistry, Faculty of Forestry, Mulawarman University, Samarinda, East Kalimantan, 75123, IndonesiaLaboratory of Forest Product Chemistry, Faculty of Forestry, Mulawarman University, Samarinda, East Kalimantan, 75123, IndonesiaDepartment of Aquaculture, Faculty of Fisheries and Marine Science, Diponegoro University, Semarang, Central Java, 50275, IndonesiaDepartment of Fisheries, Faculty of Fisheries and Marine Science, Padjajaran University, Bandung, West Java, 40600, IndonesiaBackground: The combination of some plant extracts to prevent and treat bacterial infections is gaining momentum, because of effectiveness against certain bacteria. This study aims to describe the antibacterial and immunostimulant abilities of Boesenbergia pandurata (BP), Solanum ferox (SF) and Zingiber Zerumbet (ZZ) plant extracts to treat and prevent Aeromonas hydrophila and Pseudomonas fluorescens infection on Tilapia (Oreochromis niloticus). Methods: Tilapia (initial weight 15±2 g) were injected intramuscularly (0.1 ml/fish) with a combination of A. hydrophila and P. fluorescens at a density of 1×10 5 CFU ml -1 of each bacteria. Treatment trials were performed at day 7 post-injection with each combined extract, while the prevention trial was performed by including the combined extract into the commercial diet for six and seven days prior to injection. Various extract combinations were 60 mg SF extract/kg feed with 40 mg ZZ/kg feed (SF60/ZZ40), SF50/ZZ50, BP90/SF10, and BP50/SF50. Haemato-immunological parameters were performed for four weeks. Results: In prevention trials, tilapia fed SF50/ZZ50 showed a significant increase of white and red blood cells. Similarly, significantly increased haematocrit was found in tilapia fed SF50/ZZ50 in the treatment trial but not in the prevention trial. In both trials, haemoglobin of tilapia was not affected by any combined extracts but decreased the number of bacteria. Phagocytic index, respiratory burst, lysozyme activity and survival rate of fish fed combined extracts were found significantly higher than controls. The amount of pathogenic bacteria in fish fed combined extracts was lower than the control at week 4 (P<0.05). In both trials The percentage of survival rate and relative percent survival of tilapia fed SF 50/ZZ 50, showed the optimum results compared to the other combinations. Conclusions: The combined extract in feed, especially SF50/ZZ50 has a positive effect on the tilapia's innate immune system of tilapia to treat and prevent bacterial infections.https://f1000research.com/articles/7-1847/v2 |
| spellingShingle | Esti Handayani Hardi Rudy Agung Nugroho Irawan Wijaya Kusuma Wiwin Suwinarti Agung Sudaryono Rita Rostika Borneo herbal plant extracts as a natural medication for prophylaxis and treatment of Aeromonas hydrophila and Pseudomonas fluorescens infection in tilapia (Oreochromis niloticus) [version 2; peer review: 2 approved, 1 approved with reservations] F1000Research |
| title | Borneo herbal plant extracts as a natural medication for prophylaxis and treatment of Aeromonas hydrophila and Pseudomonas fluorescens infection in tilapia (Oreochromis niloticus) [version 2; peer review: 2 approved, 1 approved with reservations] |
| title_full | Borneo herbal plant extracts as a natural medication for prophylaxis and treatment of Aeromonas hydrophila and Pseudomonas fluorescens infection in tilapia (Oreochromis niloticus) [version 2; peer review: 2 approved, 1 approved with reservations] |
| title_fullStr | Borneo herbal plant extracts as a natural medication for prophylaxis and treatment of Aeromonas hydrophila and Pseudomonas fluorescens infection in tilapia (Oreochromis niloticus) [version 2; peer review: 2 approved, 1 approved with reservations] |
| title_full_unstemmed | Borneo herbal plant extracts as a natural medication for prophylaxis and treatment of Aeromonas hydrophila and Pseudomonas fluorescens infection in tilapia (Oreochromis niloticus) [version 2; peer review: 2 approved, 1 approved with reservations] |
| title_short | Borneo herbal plant extracts as a natural medication for prophylaxis and treatment of Aeromonas hydrophila and Pseudomonas fluorescens infection in tilapia (Oreochromis niloticus) [version 2; peer review: 2 approved, 1 approved with reservations] |
| title_sort | borneo herbal plant extracts as a natural medication for prophylaxis and treatment of aeromonas hydrophila and pseudomonas fluorescens infection in tilapia oreochromis niloticus version 2 peer review 2 approved 1 approved with reservations |
| url | https://f1000research.com/articles/7-1847/v2 |
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