Fatty acid concentration of plasma, muscle, adipose and liver from beef heifers fed an encapsulated n-3 polyunsaturated fatty acid supplement
Increasing the content of polyunsaturated fat in the human diet is a priority for reducing cardiovascular disease and cancer risks. Beef has the potential to contribute to the polyunsaturated fat content in the human diet; however, ruminants cannot synthesise many long-chain fatty acids de novo; the...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-01-01
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| Series: | Animal |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1751731120300392 |
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| author | A.A. Hennessy D.A. Kenny C.J. Byrne S. Childs R.P. Ross R. Devery C. Stanton |
| author_facet | A.A. Hennessy D.A. Kenny C.J. Byrne S. Childs R.P. Ross R. Devery C. Stanton |
| author_sort | A.A. Hennessy |
| collection | DOAJ |
| description | Increasing the content of polyunsaturated fat in the human diet is a priority for reducing cardiovascular disease and cancer risks. Beef has the potential to contribute to the polyunsaturated fat content in the human diet; however, ruminants cannot synthesise many long-chain fatty acids de novo; they require dietary supplementation. The objectives of the current study were to evaluate (i) the effect of a partially rumen protected n-3 long-chain polyunsaturated fatty acid (LC-PUFA) dietary supplement on the fatty acid composition of muscle (Longissimus dorsi), adipose and liver tissues of beef heifers and (ii) the usefulness of blood plasma as a predictor of tissue concentrations of specific fatty acids. Charolais crossbred heifers (n = 20) were assigned to one of two isolipid dietary treatments namely palmitic acid (control) or an n-3 LC-PUFA supplement for a 91-day period. Blood plasma and adipose tissue samples were taken to determine the temporal effect of these diets on fatty acid composition (days 0, 10, 35 and 91), while liver and muscle samples were taken following slaughter. Dietary lipid source did not influence animal growth rate or body condition score. At day 91, the percentage differences between control and n-3 LC-PUFA heifers in concentrations of eicosapentaenoic acid were +61, +176 and +133 % in liver, muscle and adipose, respectively. For docosahexaenoic acid, at the same time point, the percentage differences were +57, +73 and +138 % for liver, muscle and adipose, respectively. Medium-to-strong positive correlation coefficients were evident for liver and plasma fatty acids, in particular, there were positive relationships with concentrations of total saturated fatty acid (SFA), total n-6 PUFA and total n-3 PUFA. This trend also extended to both the ratio of PUFA to SFA (slope (β1) = 0.56 ± 0.167, intercept (β0) = 0.56, R2 = 0.61, P < 0.05) and the ratio of n-6 to n-3 PUFA (β1 = 0.15 ± 0.054, β0 = 0.24, R2 = 0.52, P < 0.05). A strong correlation was also detected in the ratio of n-6 to n-3 in plasma and muscle tissue of heifers fed the n-3 LC-PUFA diet (β1 = 0.53 ± 0.089, β0 = −0.31, R2 = 0.83, P < 0.001). The results of this study show that the n-3 LC-PUFA can be readily increased through targeted supplementation and that plasma concentrations of n-3 LC-PUFA are useful predictors of their concentrations in a number of economically important tissues. |
| first_indexed | 2024-12-14T20:24:54Z |
| format | Article |
| id | doaj.art-6d405b83636c448aafcdfc1c62e21454 |
| institution | Directory Open Access Journal |
| issn | 1751-7311 |
| language | English |
| last_indexed | 2024-12-14T20:24:54Z |
| publishDate | 2021-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Animal |
| spelling | doaj.art-6d405b83636c448aafcdfc1c62e214542022-12-21T22:48:39ZengElsevierAnimal1751-73112021-01-01151100039Fatty acid concentration of plasma, muscle, adipose and liver from beef heifers fed an encapsulated n-3 polyunsaturated fatty acid supplementA.A. Hennessy0D.A. Kenny1C.J. Byrne2S. Childs3R.P. Ross4R. Devery5C. Stanton6Moorepark Food Research Centre, Teagasc, Moorepark, Fermoy, Co. Cork P61 C996, Ireland; National Institute for Cellular Biotechnology, Dublin City University, D09 NR58, IrelandAnimal and Bioscience Research Centre, Teagasc Grange, Dunsany, Co. Meath C15 PW93, Ireland; Corresponding author.Animal and Bioscience Research Centre, Teagasc Grange, Dunsany, Co. Meath C15 PW93, IrelandAnimal and Bioscience Research Centre, Teagasc Grange, Dunsany, Co. Meath C15 PW93, Ireland; Animal Production Research Centre, Teagasc, Mellows Campus, Athenry, Co. Galway H65 R718, IrelandMoorepark Food Research Centre, Teagasc, Moorepark, Fermoy, Co. Cork P61 C996, IrelandNational Institute for Cellular Biotechnology, Dublin City University, D09 NR58, IrelandMoorepark Food Research Centre, Teagasc, Moorepark, Fermoy, Co. Cork P61 C996, IrelandIncreasing the content of polyunsaturated fat in the human diet is a priority for reducing cardiovascular disease and cancer risks. Beef has the potential to contribute to the polyunsaturated fat content in the human diet; however, ruminants cannot synthesise many long-chain fatty acids de novo; they require dietary supplementation. The objectives of the current study were to evaluate (i) the effect of a partially rumen protected n-3 long-chain polyunsaturated fatty acid (LC-PUFA) dietary supplement on the fatty acid composition of muscle (Longissimus dorsi), adipose and liver tissues of beef heifers and (ii) the usefulness of blood plasma as a predictor of tissue concentrations of specific fatty acids. Charolais crossbred heifers (n = 20) were assigned to one of two isolipid dietary treatments namely palmitic acid (control) or an n-3 LC-PUFA supplement for a 91-day period. Blood plasma and adipose tissue samples were taken to determine the temporal effect of these diets on fatty acid composition (days 0, 10, 35 and 91), while liver and muscle samples were taken following slaughter. Dietary lipid source did not influence animal growth rate or body condition score. At day 91, the percentage differences between control and n-3 LC-PUFA heifers in concentrations of eicosapentaenoic acid were +61, +176 and +133 % in liver, muscle and adipose, respectively. For docosahexaenoic acid, at the same time point, the percentage differences were +57, +73 and +138 % for liver, muscle and adipose, respectively. Medium-to-strong positive correlation coefficients were evident for liver and plasma fatty acids, in particular, there were positive relationships with concentrations of total saturated fatty acid (SFA), total n-6 PUFA and total n-3 PUFA. This trend also extended to both the ratio of PUFA to SFA (slope (β1) = 0.56 ± 0.167, intercept (β0) = 0.56, R2 = 0.61, P < 0.05) and the ratio of n-6 to n-3 PUFA (β1 = 0.15 ± 0.054, β0 = 0.24, R2 = 0.52, P < 0.05). A strong correlation was also detected in the ratio of n-6 to n-3 in plasma and muscle tissue of heifers fed the n-3 LC-PUFA diet (β1 = 0.53 ± 0.089, β0 = −0.31, R2 = 0.83, P < 0.001). The results of this study show that the n-3 LC-PUFA can be readily increased through targeted supplementation and that plasma concentrations of n-3 LC-PUFA are useful predictors of their concentrations in a number of economically important tissues.http://www.sciencedirect.com/science/article/pii/S1751731120300392Beef cattleDocosahexaenoic acidFish oiln6:n3 ratioPolyunsaturated fatty acids |
| spellingShingle | A.A. Hennessy D.A. Kenny C.J. Byrne S. Childs R.P. Ross R. Devery C. Stanton Fatty acid concentration of plasma, muscle, adipose and liver from beef heifers fed an encapsulated n-3 polyunsaturated fatty acid supplement Animal Beef cattle Docosahexaenoic acid Fish oil n6:n3 ratio Polyunsaturated fatty acids |
| title | Fatty acid concentration of plasma, muscle, adipose and liver from beef heifers fed an encapsulated n-3 polyunsaturated fatty acid supplement |
| title_full | Fatty acid concentration of plasma, muscle, adipose and liver from beef heifers fed an encapsulated n-3 polyunsaturated fatty acid supplement |
| title_fullStr | Fatty acid concentration of plasma, muscle, adipose and liver from beef heifers fed an encapsulated n-3 polyunsaturated fatty acid supplement |
| title_full_unstemmed | Fatty acid concentration of plasma, muscle, adipose and liver from beef heifers fed an encapsulated n-3 polyunsaturated fatty acid supplement |
| title_short | Fatty acid concentration of plasma, muscle, adipose and liver from beef heifers fed an encapsulated n-3 polyunsaturated fatty acid supplement |
| title_sort | fatty acid concentration of plasma muscle adipose and liver from beef heifers fed an encapsulated n 3 polyunsaturated fatty acid supplement |
| topic | Beef cattle Docosahexaenoic acid Fish oil n6:n3 ratio Polyunsaturated fatty acids |
| url | http://www.sciencedirect.com/science/article/pii/S1751731120300392 |
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