Phytochemical Screening and Antioxidant and Cytotoxic Effects of <i>Acacia macrostachya</i>
<i>Acacia macrostachya</i> is used in Burkina Faso folk medicine for the treatment of inflammation and cancer. The purpose of this study was to evaluate the antioxidant and cytotoxic effects of this plant. The cytotoxic effects of root (dichloromethane <b>B1</b> and methanol...
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MDPI AG
2021-07-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/10/7/1353 |
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| author | Hamidou Têeda Ganamé Yssouf Karanga Issa Tapsoba Mario Dicato Marc F. Diederich Claudia Cerella Richard Wamtinga Sawadogo |
| author_facet | Hamidou Têeda Ganamé Yssouf Karanga Issa Tapsoba Mario Dicato Marc F. Diederich Claudia Cerella Richard Wamtinga Sawadogo |
| author_sort | Hamidou Têeda Ganamé |
| collection | DOAJ |
| description | <i>Acacia macrostachya</i> is used in Burkina Faso folk medicine for the treatment of inflammation and cancer. The purpose of this study was to evaluate the antioxidant and cytotoxic effects of this plant. The cytotoxic effects of root (dichloromethane <b>B1</b> and methanol <b>B2</b>) and stem (dichloromethane <b>B3</b> and methanol <b>B4</b>) bark extracts of <i>A. macrostachya</i> were assessed on chronic K562 and acute U937 myeloid leukemia cancer cells using trypan blue, Hoechst, and MitoTracker Red staining methods. The antioxidant content of extracts was evaluated using DPPH (2,2-diphenyl-1-picryl-hydrazyl) and FRAP (ferric reducing antioxidant power) methods. The root bark extracts <b>B1</b> and <b>B2</b> of <i>A. macrostachya</i> demonstrated higher cytotoxicity with IC<sub>50</sub> values in a low µg/mL range on both U937 and K562 cells, while the stem bark <b>B4</b> extract selectively affected U937 cells. Overall, healthy proliferating peripheral blood mononuclear cells (pPBMCs) were not or barely impacted in the range of concentrations cytotoxic to cancer cells. In addition, <i>A. macrostachya</i> exhibited significant antioxidant content with 646.06 and 428.08 µg ET/mg of extract for the <b>B4</b> and <b>B2</b> extracts, respectively. Phytochemical screening showed the presence of flavonoids, tannins, alkaloids, and terpenoids/steroids. The results of this study highlight the interest of <i>A. macrostachya</i> extracts for the isolation of anticancer molecules. |
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| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T09:28:20Z |
| publishDate | 2021-07-01 |
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| record_format | Article |
| series | Plants |
| spelling | doaj.art-6d4100fd6f3543308363ef3b1b540b442023-11-22T04:42:50ZengMDPI AGPlants2223-77472021-07-01107135310.3390/plants10071353Phytochemical Screening and Antioxidant and Cytotoxic Effects of <i>Acacia macrostachya</i>Hamidou Têeda Ganamé0Yssouf Karanga1Issa Tapsoba2Mario Dicato3Marc F. Diederich4Claudia Cerella5Richard Wamtinga Sawadogo6Laboratoire de Chimie Analytique, Environnementale et Bio-Organique (LCAEBiO), Université Joseph KI-ZERBO, Ouagadougou 03 BP 7021, Burkina FasoLaboratoire de Chimie Analytique, Environnementale et Bio-Organique (LCAEBiO), Université Joseph KI-ZERBO, Ouagadougou 03 BP 7021, Burkina FasoLaboratoire de Chimie Analytique, Environnementale et Bio-Organique (LCAEBiO), Université Joseph KI-ZERBO, Ouagadougou 03 BP 7021, Burkina FasoLaboratoire de Biologie Moléculaire et Cellulaire du Cancer (LBMCC), Hôpital Kirchberg, L-2540 Luxembourg, LuxembourgCollege of Pharmacy, Seoul National University, Seoul 08826, KoreaLaboratoire de Biologie Moléculaire et Cellulaire du Cancer (LBMCC), Hôpital Kirchberg, L-2540 Luxembourg, LuxembourgInstitut de Recherche en Sciences de la Santé (IRSS/CNRST), Ouagadougou 03 BP 7192, Burkina Faso<i>Acacia macrostachya</i> is used in Burkina Faso folk medicine for the treatment of inflammation and cancer. The purpose of this study was to evaluate the antioxidant and cytotoxic effects of this plant. The cytotoxic effects of root (dichloromethane <b>B1</b> and methanol <b>B2</b>) and stem (dichloromethane <b>B3</b> and methanol <b>B4</b>) bark extracts of <i>A. macrostachya</i> were assessed on chronic K562 and acute U937 myeloid leukemia cancer cells using trypan blue, Hoechst, and MitoTracker Red staining methods. The antioxidant content of extracts was evaluated using DPPH (2,2-diphenyl-1-picryl-hydrazyl) and FRAP (ferric reducing antioxidant power) methods. The root bark extracts <b>B1</b> and <b>B2</b> of <i>A. macrostachya</i> demonstrated higher cytotoxicity with IC<sub>50</sub> values in a low µg/mL range on both U937 and K562 cells, while the stem bark <b>B4</b> extract selectively affected U937 cells. Overall, healthy proliferating peripheral blood mononuclear cells (pPBMCs) were not or barely impacted in the range of concentrations cytotoxic to cancer cells. In addition, <i>A. macrostachya</i> exhibited significant antioxidant content with 646.06 and 428.08 µg ET/mg of extract for the <b>B4</b> and <b>B2</b> extracts, respectively. Phytochemical screening showed the presence of flavonoids, tannins, alkaloids, and terpenoids/steroids. The results of this study highlight the interest of <i>A. macrostachya</i> extracts for the isolation of anticancer molecules.https://www.mdpi.com/2223-7747/10/7/1353<i>Acacia macrostachya</i>antioxidantcytotoxicitycancerleukemialymphoma |
| spellingShingle | Hamidou Têeda Ganamé Yssouf Karanga Issa Tapsoba Mario Dicato Marc F. Diederich Claudia Cerella Richard Wamtinga Sawadogo Phytochemical Screening and Antioxidant and Cytotoxic Effects of <i>Acacia macrostachya</i> Plants <i>Acacia macrostachya</i> antioxidant cytotoxicity cancer leukemia lymphoma |
| title | Phytochemical Screening and Antioxidant and Cytotoxic Effects of <i>Acacia macrostachya</i> |
| title_full | Phytochemical Screening and Antioxidant and Cytotoxic Effects of <i>Acacia macrostachya</i> |
| title_fullStr | Phytochemical Screening and Antioxidant and Cytotoxic Effects of <i>Acacia macrostachya</i> |
| title_full_unstemmed | Phytochemical Screening and Antioxidant and Cytotoxic Effects of <i>Acacia macrostachya</i> |
| title_short | Phytochemical Screening and Antioxidant and Cytotoxic Effects of <i>Acacia macrostachya</i> |
| title_sort | phytochemical screening and antioxidant and cytotoxic effects of i acacia macrostachya i |
| topic | <i>Acacia macrostachya</i> antioxidant cytotoxicity cancer leukemia lymphoma |
| url | https://www.mdpi.com/2223-7747/10/7/1353 |
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