Developing EST-SSR Markers for Identifying and Evaluating <i>Asparagus</i> Germplasm Resources Based on Transcriptome Sequences
<i>Radix asparagi</i> is the dried root tuber of the Liliaceae plant <i>Asparagus cochinchinensis</i> (Lour.) Merr., which is a major Chinese medicinal herb with high medicinal and edible value in China. The planting area of <i>A. cochinchinensis</i> is extensive,...
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MDPI AG
2024-01-01
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| Series: | Horticulturae |
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| Online Access: | https://www.mdpi.com/2311-7524/10/2/121 |
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| author | Dan Liu Feili Yan Changmei Liu Aimeng Chen Jiahui Wu Ma Yu Xiangyang Lyu |
| author_facet | Dan Liu Feili Yan Changmei Liu Aimeng Chen Jiahui Wu Ma Yu Xiangyang Lyu |
| author_sort | Dan Liu |
| collection | DOAJ |
| description | <i>Radix asparagi</i> is the dried root tuber of the Liliaceae plant <i>Asparagus cochinchinensis</i> (Lour.) Merr., which is a major Chinese medicinal herb with high medicinal and edible value in China. The planting area of <i>A. cochinchinensis</i> is extensive, and there is blind introduction in various regions, leading to confusion the origin of <i>Radix asparagi</i> and impure germplasm. This study conducted morphological and karyotype analyses on cultivated <i>Asparagus</i> resources from seven main production areas in China and developed SSR molecular markers suitable for the identification of <i>Asparagus</i> germplasm resources based on the transcriptome sequencing results. The morphological results indicate that in addition to <i>A. cochinchinensis</i> (Lour.) Merr., recorded in the <i>Pharmacopoeia of the People’s Republic of China</i>, there are also <i>A. taliensis</i> Wang et Tang and <i>A. lycopodineus</i> (Baker) Wang et Tang cultivated in China. All the tested <i>Asparagus</i> resources were diploid and had 20 chromosomes. A total of 8841 single genes containing SSR loci were identified using transcriptome sequencing of Neijiang <i>Asparagus</i>, including 761 SSR loci with trinucleotide repeat units. One hundred pairs of SSR primers were randomly designed from the trinucleotide repeat loci for PCR and polymorphism verification, and ten pairs were selected for identification of <i>Asparagus</i> germplasm resources. The genetic diversity results of ten pairs of primers in seven <i>Asparagus</i>-producing regions were consistent with the morphological identification. This study provides technical support for the comprehensive evaluation and utilization of <i>Asparagus</i> germplasm resources. |
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| institution | Directory Open Access Journal |
| issn | 2311-7524 |
| language | English |
| last_indexed | 2024-03-07T22:30:36Z |
| publishDate | 2024-01-01 |
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| series | Horticulturae |
| spelling | doaj.art-6d42a94825504676942f9c62e518f02b2024-02-23T15:18:37ZengMDPI AGHorticulturae2311-75242024-01-0110212110.3390/horticulturae10020121Developing EST-SSR Markers for Identifying and Evaluating <i>Asparagus</i> Germplasm Resources Based on Transcriptome SequencesDan Liu0Feili Yan1Changmei Liu2Aimeng Chen3Jiahui Wu4Ma Yu5Xiangyang Lyu6Neijiang Academy of Agricultural Sciences of Sichuan Province, Neijiang 641000, ChinaNeijiang Academy of Agricultural Sciences of Sichuan Province, Neijiang 641000, ChinaSchool of Biotechnology, Jiangnan University, Wuxi 214122, ChinaNeijiang Academy of Agricultural Sciences of Sichuan Province, Neijiang 641000, ChinaNeijiang Academy of Agricultural Sciences of Sichuan Province, Neijiang 641000, ChinaSchool of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, ChinaNeijiang Academy of Agricultural Sciences of Sichuan Province, Neijiang 641000, China<i>Radix asparagi</i> is the dried root tuber of the Liliaceae plant <i>Asparagus cochinchinensis</i> (Lour.) Merr., which is a major Chinese medicinal herb with high medicinal and edible value in China. The planting area of <i>A. cochinchinensis</i> is extensive, and there is blind introduction in various regions, leading to confusion the origin of <i>Radix asparagi</i> and impure germplasm. This study conducted morphological and karyotype analyses on cultivated <i>Asparagus</i> resources from seven main production areas in China and developed SSR molecular markers suitable for the identification of <i>Asparagus</i> germplasm resources based on the transcriptome sequencing results. The morphological results indicate that in addition to <i>A. cochinchinensis</i> (Lour.) Merr., recorded in the <i>Pharmacopoeia of the People’s Republic of China</i>, there are also <i>A. taliensis</i> Wang et Tang and <i>A. lycopodineus</i> (Baker) Wang et Tang cultivated in China. All the tested <i>Asparagus</i> resources were diploid and had 20 chromosomes. A total of 8841 single genes containing SSR loci were identified using transcriptome sequencing of Neijiang <i>Asparagus</i>, including 761 SSR loci with trinucleotide repeat units. One hundred pairs of SSR primers were randomly designed from the trinucleotide repeat loci for PCR and polymorphism verification, and ten pairs were selected for identification of <i>Asparagus</i> germplasm resources. The genetic diversity results of ten pairs of primers in seven <i>Asparagus</i>-producing regions were consistent with the morphological identification. This study provides technical support for the comprehensive evaluation and utilization of <i>Asparagus</i> germplasm resources.https://www.mdpi.com/2311-7524/10/2/121<i>Radix asparagi</i>traitschromosomestranscriptomeSSR markergenetic diversity |
| spellingShingle | Dan Liu Feili Yan Changmei Liu Aimeng Chen Jiahui Wu Ma Yu Xiangyang Lyu Developing EST-SSR Markers for Identifying and Evaluating <i>Asparagus</i> Germplasm Resources Based on Transcriptome Sequences Horticulturae <i>Radix asparagi</i> traits chromosomes transcriptome SSR marker genetic diversity |
| title | Developing EST-SSR Markers for Identifying and Evaluating <i>Asparagus</i> Germplasm Resources Based on Transcriptome Sequences |
| title_full | Developing EST-SSR Markers for Identifying and Evaluating <i>Asparagus</i> Germplasm Resources Based on Transcriptome Sequences |
| title_fullStr | Developing EST-SSR Markers for Identifying and Evaluating <i>Asparagus</i> Germplasm Resources Based on Transcriptome Sequences |
| title_full_unstemmed | Developing EST-SSR Markers for Identifying and Evaluating <i>Asparagus</i> Germplasm Resources Based on Transcriptome Sequences |
| title_short | Developing EST-SSR Markers for Identifying and Evaluating <i>Asparagus</i> Germplasm Resources Based on Transcriptome Sequences |
| title_sort | developing est ssr markers for identifying and evaluating i asparagus i germplasm resources based on transcriptome sequences |
| topic | <i>Radix asparagi</i> traits chromosomes transcriptome SSR marker genetic diversity |
| url | https://www.mdpi.com/2311-7524/10/2/121 |
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