Effects of triclosan on bacterial community composition and 'Vibrio' populations in natural seawater microcosms
Pharmaceuticals and personal care products, including antimicrobials, can be found at trace levels in treated wastewater effluent. Impacts of chemical contaminants on coastal aquatic microbial community structure and pathogen abundance are unknown despite the potential for selection through antimicr...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BioOne
2017-05-01
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| Series: | Elementa: Science of the Anthropocene |
| Subjects: | |
| Online Access: | https://www.elementascience.org/articles/141 |
| _version_ | 1819065765189386240 |
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| author | Keri Ann Lydon Donna A. Glinski Jason R. Westrich W. Matthew Henderson Erin K. Lipp |
| author_facet | Keri Ann Lydon Donna A. Glinski Jason R. Westrich W. Matthew Henderson Erin K. Lipp |
| author_sort | Keri Ann Lydon |
| collection | DOAJ |
| description | Pharmaceuticals and personal care products, including antimicrobials, can be found at trace levels in treated wastewater effluent. Impacts of chemical contaminants on coastal aquatic microbial community structure and pathogen abundance are unknown despite the potential for selection through antimicrobial resistance. In particular, 'Vibrio', a marine bacterial genus that includes several human pathogens, displays resistance to the ubiquitous antimicrobial compound triclosan. Here we demonstrated through use of natural seawater microcosms that triclosan (at a concentration of ~5 ppm) can induce a significant 'Vibrio' growth response (68–1,700 fold increases) in comparison with no treatment controls for three distinct coastal ecosystems: Looe Key Reef (Florida Keys National Marine Sanctuary), Doctors Arm Canal (Big Pine Key, FL), and Clam Bank Landing (North Inlet Estuary, Georgetown, SC). Additionally, microbial community analysis by 16 S rRNA gene sequencing for Looe Key Reef showed distinct changes in microbial community structure with exposure to 5 ppm triclosan, with increases observed in the relative abundance of 'Vibrio'naceae (17-fold), Pseudoalteromonadaceae (65-fold), Alteromonadaceae (108-fold), Colwelliaceae (430-fold), and Oceanospirillaceae (1,494-fold). While the triclosan doses tested were above concentrations typically observed in coastal surface waters, results identify bacterial families that are potentially resistant to triclosan and/or adapted to use triclosan as a carbon source. The results further suggest the potential for selection of 'Vibrio' in coastal environments, especially sediments, where triclosan may accumulate at high levels. |
| first_indexed | 2024-12-21T15:51:40Z |
| format | Article |
| id | doaj.art-6d44ac79b0b74262be802b52b4c99092 |
| institution | Directory Open Access Journal |
| issn | 2325-1026 |
| language | English |
| last_indexed | 2024-12-21T15:51:40Z |
| publishDate | 2017-05-01 |
| publisher | BioOne |
| record_format | Article |
| series | Elementa: Science of the Anthropocene |
| spelling | doaj.art-6d44ac79b0b74262be802b52b4c990922022-12-21T18:58:12ZengBioOneElementa: Science of the Anthropocene2325-10262017-05-01510.1525/elementa.141164Effects of triclosan on bacterial community composition and 'Vibrio' populations in natural seawater microcosmsKeri Ann Lydon0Donna A. Glinski1Jason R. Westrich2W. Matthew Henderson3Erin K. Lipp4Department of Environmental Health Science, University of Georgia, Athens, GeorgiaDepartment of Environmental Health Science, University of Georgia; and Oak Ridge Institute of Science and Education, U.S. Environmental Protection Agency, Athens, GeorgiaDepartment of Environmental Health Science, University of Georgia, Athens, GeorgiaU.S. Environmental Protection Agency, Office of Research and Development, NERL/EMMD, Athens, GeorgiaDepartment of Environmental Health Science, University of Georgia, Athens, GeorgiaPharmaceuticals and personal care products, including antimicrobials, can be found at trace levels in treated wastewater effluent. Impacts of chemical contaminants on coastal aquatic microbial community structure and pathogen abundance are unknown despite the potential for selection through antimicrobial resistance. In particular, 'Vibrio', a marine bacterial genus that includes several human pathogens, displays resistance to the ubiquitous antimicrobial compound triclosan. Here we demonstrated through use of natural seawater microcosms that triclosan (at a concentration of ~5 ppm) can induce a significant 'Vibrio' growth response (68–1,700 fold increases) in comparison with no treatment controls for three distinct coastal ecosystems: Looe Key Reef (Florida Keys National Marine Sanctuary), Doctors Arm Canal (Big Pine Key, FL), and Clam Bank Landing (North Inlet Estuary, Georgetown, SC). Additionally, microbial community analysis by 16 S rRNA gene sequencing for Looe Key Reef showed distinct changes in microbial community structure with exposure to 5 ppm triclosan, with increases observed in the relative abundance of 'Vibrio'naceae (17-fold), Pseudoalteromonadaceae (65-fold), Alteromonadaceae (108-fold), Colwelliaceae (430-fold), and Oceanospirillaceae (1,494-fold). While the triclosan doses tested were above concentrations typically observed in coastal surface waters, results identify bacterial families that are potentially resistant to triclosan and/or adapted to use triclosan as a carbon source. The results further suggest the potential for selection of 'Vibrio' in coastal environments, especially sediments, where triclosan may accumulate at high levels.https://www.elementascience.org/articles/141TriclosanVibrioEmerging ContaminantCoastalResistance |
| spellingShingle | Keri Ann Lydon Donna A. Glinski Jason R. Westrich W. Matthew Henderson Erin K. Lipp Effects of triclosan on bacterial community composition and 'Vibrio' populations in natural seawater microcosms Elementa: Science of the Anthropocene Triclosan Vibrio Emerging Contaminant Coastal Resistance |
| title | Effects of triclosan on bacterial community composition and 'Vibrio' populations in natural seawater microcosms |
| title_full | Effects of triclosan on bacterial community composition and 'Vibrio' populations in natural seawater microcosms |
| title_fullStr | Effects of triclosan on bacterial community composition and 'Vibrio' populations in natural seawater microcosms |
| title_full_unstemmed | Effects of triclosan on bacterial community composition and 'Vibrio' populations in natural seawater microcosms |
| title_short | Effects of triclosan on bacterial community composition and 'Vibrio' populations in natural seawater microcosms |
| title_sort | effects of triclosan on bacterial community composition and vibrio populations in natural seawater microcosms |
| topic | Triclosan Vibrio Emerging Contaminant Coastal Resistance |
| url | https://www.elementascience.org/articles/141 |
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