A Truncated TIR-NBS Protein TN10 Pairs with Two Clustered TIR-NBS-LRR Immune Receptors and Contributes to Plant Immunity in <i>Arabidopsis</i>
The encoding genes of plant intracellular nucleotide-binding site (NBS) and leucine-rich repeat (LRR) domain receptors (NLRs) often exist in the form of a gene cluster. Several recent studies demonstrated that the truncated Toll/interleukin-1 receptor-NBS (TIR-NBS) proteins play important roles in i...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-04-01
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| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/22/8/4004 |
| Summary: | The encoding genes of plant intracellular nucleotide-binding site (NBS) and leucine-rich repeat (LRR) domain receptors (NLRs) often exist in the form of a gene cluster. Several recent studies demonstrated that the truncated Toll/interleukin-1 receptor-NBS (TIR-NBS) proteins play important roles in immunity. In this study, we identified a large <i>TN</i> gene cluster on <i>Arabidopsis</i> ecotype Col-0 chromosome 1, which included nine <i>TN</i> genes, <i>TN4</i> to <i>TN12</i>. Interestingly, this cluster also contained two typical <i>TIR-NBS-LRR</i> genes: <i>At1g72840</i> and <i>At1g72860</i> (hereinafter referred to as <i>TNL40</i> and <i>TNL60,</i> respectively), which formed head-to-head genomic arrangement with <i>TN4</i> to <i>TN12</i>. However, the functions of these <i>TN</i> and <i>TNL</i> genes in this cluster are still unknown. Here, we showed that the TIR domains of both TNL40 and TNL60 associated with TN10 specifically. Furthermore, both TNL40TIR and TNL60TIR induced cell death in <i>Nicotiana tabacum</i> leaves. Subcellular localization showed that TNL40 mainly localized in the cytoplasm, whereas TNL60 and TN10 localized in both the cytoplasm and nucleus. Additionally, the expression of <i>TNL40</i>, <i>TNL60</i>, and <i>TN10</i> were co-regulated after inoculated with bacterial pathogens. Taken together, our study indicates that the truncated TIR-NBS protein TN10 associates with two clustered TNL immune receptors, and may work together in plant disease resistance |
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| ISSN: | 1661-6596 1422-0067 |