Synergistic Improvement of Carbohydrate and Lignin Processability by Biomimicking Biomass Processing
The sustainability and economic feasibility of modern biorefinery depend on the efficient processing of both carbohydrate and lignin fractions for value-added products. By mimicking the biomass degradation process in white-rote fungi, a tailored two-step fractionation process was developed to maximi...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-02-01
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| Series: | Frontiers in Energy Research |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fenrg.2020.00194/full |
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| author | Man Li Man Li Zhi-Hua Liu Zhi-Hua Liu Naijia Hao Michelle L. Olson Qiang Li Qiang Li Samarthya Bhagia Somnath Shinde Katy C. Kao Arthur J. Ragauskas Arthur J. Ragauskas Arthur J. Ragauskas Shangxian Xie Shangxian Xie Shangxian Xie Joshua S. Yuan Joshua S. Yuan |
| author_facet | Man Li Man Li Zhi-Hua Liu Zhi-Hua Liu Naijia Hao Michelle L. Olson Qiang Li Qiang Li Samarthya Bhagia Somnath Shinde Katy C. Kao Arthur J. Ragauskas Arthur J. Ragauskas Arthur J. Ragauskas Shangxian Xie Shangxian Xie Shangxian Xie Joshua S. Yuan Joshua S. Yuan |
| author_sort | Man Li |
| collection | DOAJ |
| description | The sustainability and economic feasibility of modern biorefinery depend on the efficient processing of both carbohydrate and lignin fractions for value-added products. By mimicking the biomass degradation process in white-rote fungi, a tailored two-step fractionation process was developed to maximize the sugar release from switchgrass biomass and to optimize the lignin processability for bioconversion. Biomimicking biomass processing using Formic Acid: Fenton: Organosolv (F2O) and achieved high processability for both carbohydrate and lignin. Specifically, switchgrass pretreated by the F2O process had 99.6% of the theoretical yield for glucose release. The fractionated lignin was also readily processable by fermentation via Rhodococcus opacus PD630 with a lipid yield of 1.16 g/L. Scanning electron microscope analysis confirmed the fragmentation of switchgrass fiber and the cell wall deconstruction by the F2O process. 2D-HSQC NMR further revealed the cleavage of aryl ether linkages (β-O-4) in lignin components. These results revealed the mechanisms for efficient sugar release and lignin bioconversion. The F2O process demonstrated effective mimicking of natural biomass utilization system and paved a new path for improving the lignin and carbohydrate processability in next generation lignocellulosic biorefinery. |
| first_indexed | 2024-12-17T05:42:43Z |
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| institution | Directory Open Access Journal |
| issn | 2296-598X |
| language | English |
| last_indexed | 2024-12-17T05:42:43Z |
| publishDate | 2021-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Energy Research |
| spelling | doaj.art-6d4c3ec93ea14dfbb40de0c0358a8b5a2022-12-21T22:01:24ZengFrontiers Media S.A.Frontiers in Energy Research2296-598X2021-02-01810.3389/fenrg.2020.00194541924Synergistic Improvement of Carbohydrate and Lignin Processability by Biomimicking Biomass ProcessingMan Li0Man Li1Zhi-Hua Liu2Zhi-Hua Liu3Naijia Hao4Michelle L. Olson5Qiang Li6Qiang Li7Samarthya Bhagia8Somnath Shinde9Katy C. Kao10Arthur J. Ragauskas11Arthur J. Ragauskas12Arthur J. Ragauskas13Shangxian Xie14Shangxian Xie15Shangxian Xie16Joshua S. Yuan17Joshua S. Yuan18Synthetic and Systems Biology Innovation Hub, Texas A&M University, College Station, TX, United StatesDepartment of Plant Pathology and Microbiology, Texas A&M University, College Station, TX, United StatesSynthetic and Systems Biology Innovation Hub, Texas A&M University, College Station, TX, United StatesDepartment of Plant Pathology and Microbiology, Texas A&M University, College Station, TX, United StatesDepartment of Chemical & Biomolecular Engineering, The University of Tennessee, Knoxville, Knoxville, TN, United StatesDepartment of Chemical Engineering, Texas A&M University, College Station, TX, United StatesSynthetic and Systems Biology Innovation Hub, Texas A&M University, College Station, TX, United StatesDepartment of Plant Pathology and Microbiology, Texas A&M University, College Station, TX, United StatesJoint Institute of Biological Sciences, Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, United StatesDepartment of Chemical & Biomolecular Engineering, The University of Tennessee, Knoxville, Knoxville, TN, United StatesDepartment of Chemical Engineering, Texas A&M University, College Station, TX, United StatesDepartment of Chemical & Biomolecular Engineering, The University of Tennessee, Knoxville, Knoxville, TN, United StatesJoint Institute of Biological Sciences, Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, United StatesDepartment of Forestry, Wildlife, and Fisheries, Center for Renewable Carbon, The University of Tennessee Institute of Agriculture, Knoxville, TN, United StatesSynthetic and Systems Biology Innovation Hub, Texas A&M University, College Station, TX, United StatesDepartment of Plant Pathology and Microbiology, Texas A&M University, College Station, TX, United StatesCollege of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, ChinaSynthetic and Systems Biology Innovation Hub, Texas A&M University, College Station, TX, United StatesDepartment of Plant Pathology and Microbiology, Texas A&M University, College Station, TX, United StatesThe sustainability and economic feasibility of modern biorefinery depend on the efficient processing of both carbohydrate and lignin fractions for value-added products. By mimicking the biomass degradation process in white-rote fungi, a tailored two-step fractionation process was developed to maximize the sugar release from switchgrass biomass and to optimize the lignin processability for bioconversion. Biomimicking biomass processing using Formic Acid: Fenton: Organosolv (F2O) and achieved high processability for both carbohydrate and lignin. Specifically, switchgrass pretreated by the F2O process had 99.6% of the theoretical yield for glucose release. The fractionated lignin was also readily processable by fermentation via Rhodococcus opacus PD630 with a lipid yield of 1.16 g/L. Scanning electron microscope analysis confirmed the fragmentation of switchgrass fiber and the cell wall deconstruction by the F2O process. 2D-HSQC NMR further revealed the cleavage of aryl ether linkages (β-O-4) in lignin components. These results revealed the mechanisms for efficient sugar release and lignin bioconversion. The F2O process demonstrated effective mimicking of natural biomass utilization system and paved a new path for improving the lignin and carbohydrate processability in next generation lignocellulosic biorefinery.https://www.frontiersin.org/articles/10.3389/fenrg.2020.00194/fulllignocellulosic biomasslipidorganosolvFentonformic acidpretreatment |
| spellingShingle | Man Li Man Li Zhi-Hua Liu Zhi-Hua Liu Naijia Hao Michelle L. Olson Qiang Li Qiang Li Samarthya Bhagia Somnath Shinde Katy C. Kao Arthur J. Ragauskas Arthur J. Ragauskas Arthur J. Ragauskas Shangxian Xie Shangxian Xie Shangxian Xie Joshua S. Yuan Joshua S. Yuan Synergistic Improvement of Carbohydrate and Lignin Processability by Biomimicking Biomass Processing Frontiers in Energy Research lignocellulosic biomass lipid organosolv Fenton formic acid pretreatment |
| title | Synergistic Improvement of Carbohydrate and Lignin Processability by Biomimicking Biomass Processing |
| title_full | Synergistic Improvement of Carbohydrate and Lignin Processability by Biomimicking Biomass Processing |
| title_fullStr | Synergistic Improvement of Carbohydrate and Lignin Processability by Biomimicking Biomass Processing |
| title_full_unstemmed | Synergistic Improvement of Carbohydrate and Lignin Processability by Biomimicking Biomass Processing |
| title_short | Synergistic Improvement of Carbohydrate and Lignin Processability by Biomimicking Biomass Processing |
| title_sort | synergistic improvement of carbohydrate and lignin processability by biomimicking biomass processing |
| topic | lignocellulosic biomass lipid organosolv Fenton formic acid pretreatment |
| url | https://www.frontiersin.org/articles/10.3389/fenrg.2020.00194/full |
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