Anti-Aging Properties of Chitosan-Based Hydrogels Rich in Bilberry Fruit Extract
Photoaging is a process related to an increased level of reactive oxygen species (ROS). Polyphenols can scavenge free radicals in the body, which can delay skin aging. Therefore, our work aimed to prepare a biologically active extract from dry fruits of <i>Vaccinium myrtillus</i> or <...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-01-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/13/1/105 |
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| author | Elżbieta Studzińska-Sroka Magdalena Paczkowska-Walendowska Cansu Erdem Jarosław Paluszczak Robert Kleszcz Marta Hoszman-Kulisz Judyta Cielecka-Piontek |
| author_facet | Elżbieta Studzińska-Sroka Magdalena Paczkowska-Walendowska Cansu Erdem Jarosław Paluszczak Robert Kleszcz Marta Hoszman-Kulisz Judyta Cielecka-Piontek |
| author_sort | Elżbieta Studzińska-Sroka |
| collection | DOAJ |
| description | Photoaging is a process related to an increased level of reactive oxygen species (ROS). Polyphenols can scavenge free radicals in the body, which can delay skin aging. Therefore, our work aimed to prepare a biologically active extract from dry fruits of <i>Vaccinium myrtillus</i> or <i>Vaccinium corymbosum</i> and use it for the preparation of hydrogels for topical application. Therefore, eight different extracts (using <i>V. myrtillus</i> and <i>V. corymbosum</i> and different extraction mixtures: methanol, methanol–water 1:1, water, acetone–water 1:1) were prepared and their phytochemical (total polyphenolic content, total flavonoid content, total anthocyanin content) and biological properties (antioxidant, anti-hyaluronidase, and anti-tyrosinase activity) were assessed. Cytotoxicity towards HaCaT keratinocytes was also determined. Based on the results, the acetone–water extract from <i>V. myrtillus</i> was selected for further study. Using the Design of Experiments approach, chitosan-based hydrogels with bilberry fruit extract were prepared. The content of extract and chitosan were selected as independent factors. The activity of hydrogels depended on the extract content; however, the enzyme-inhibiting (anti-hyaluronidase and anti-tyrosinase) activity resulted from the presence of both the extract and chitosan. Increased concentration of chitosan in the hydrogel base led to increased viscosity of the hydrogel and, consequently, a slower release of active compounds. To get optimal hydrogel characteristics, 1% extract and 2.5% MMW chitosan were utilized. The research suggests the validity of using bilberry fruit extracts in topical preparations with anti-aging properties. |
| first_indexed | 2024-03-08T11:06:20Z |
| format | Article |
| id | doaj.art-6d4d9f44dad849849c5dbc9678f01308 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-08T11:06:20Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-6d4d9f44dad849849c5dbc9678f013082024-01-26T14:41:56ZengMDPI AGAntioxidants2076-39212024-01-0113110510.3390/antiox13010105Anti-Aging Properties of Chitosan-Based Hydrogels Rich in Bilberry Fruit ExtractElżbieta Studzińska-Sroka0Magdalena Paczkowska-Walendowska1Cansu Erdem2Jarosław Paluszczak3Robert Kleszcz4Marta Hoszman-Kulisz5Judyta Cielecka-Piontek6Department of Pharmacognosy and Biomaterials, Poznan University of Medical Sciences, Rokietnicka 3 Str, 60-806 Poznań, PolandDepartment of Pharmacognosy and Biomaterials, Poznan University of Medical Sciences, Rokietnicka 3 Str, 60-806 Poznań, PolandDepartment Pharmaceutical Chemistry, Ege Üniversitesi, 35040 İzmir, TurkeyDepartment of Pharmaceutical Biochemistry, Poznan University of Medical Sciences, Rokietnicka 3 Str, 60-806 Poznań, PolandDepartment of Pharmaceutical Biochemistry, Poznan University of Medical Sciences, Rokietnicka 3 Str, 60-806 Poznań, PolandDepartment of Pharmacognosy and Biomaterials, Poznan University of Medical Sciences, Rokietnicka 3 Str, 60-806 Poznań, PolandDepartment of Pharmacognosy and Biomaterials, Poznan University of Medical Sciences, Rokietnicka 3 Str, 60-806 Poznań, PolandPhotoaging is a process related to an increased level of reactive oxygen species (ROS). Polyphenols can scavenge free radicals in the body, which can delay skin aging. Therefore, our work aimed to prepare a biologically active extract from dry fruits of <i>Vaccinium myrtillus</i> or <i>Vaccinium corymbosum</i> and use it for the preparation of hydrogels for topical application. Therefore, eight different extracts (using <i>V. myrtillus</i> and <i>V. corymbosum</i> and different extraction mixtures: methanol, methanol–water 1:1, water, acetone–water 1:1) were prepared and their phytochemical (total polyphenolic content, total flavonoid content, total anthocyanin content) and biological properties (antioxidant, anti-hyaluronidase, and anti-tyrosinase activity) were assessed. Cytotoxicity towards HaCaT keratinocytes was also determined. Based on the results, the acetone–water extract from <i>V. myrtillus</i> was selected for further study. Using the Design of Experiments approach, chitosan-based hydrogels with bilberry fruit extract were prepared. The content of extract and chitosan were selected as independent factors. The activity of hydrogels depended on the extract content; however, the enzyme-inhibiting (anti-hyaluronidase and anti-tyrosinase) activity resulted from the presence of both the extract and chitosan. Increased concentration of chitosan in the hydrogel base led to increased viscosity of the hydrogel and, consequently, a slower release of active compounds. To get optimal hydrogel characteristics, 1% extract and 2.5% MMW chitosan were utilized. The research suggests the validity of using bilberry fruit extracts in topical preparations with anti-aging properties.https://www.mdpi.com/2076-3921/13/1/105bilberry fruitsblueberry fruitanti-aging potentialtopical preparationcosmeticschitosan |
| spellingShingle | Elżbieta Studzińska-Sroka Magdalena Paczkowska-Walendowska Cansu Erdem Jarosław Paluszczak Robert Kleszcz Marta Hoszman-Kulisz Judyta Cielecka-Piontek Anti-Aging Properties of Chitosan-Based Hydrogels Rich in Bilberry Fruit Extract Antioxidants bilberry fruits blueberry fruit anti-aging potential topical preparation cosmetics chitosan |
| title | Anti-Aging Properties of Chitosan-Based Hydrogels Rich in Bilberry Fruit Extract |
| title_full | Anti-Aging Properties of Chitosan-Based Hydrogels Rich in Bilberry Fruit Extract |
| title_fullStr | Anti-Aging Properties of Chitosan-Based Hydrogels Rich in Bilberry Fruit Extract |
| title_full_unstemmed | Anti-Aging Properties of Chitosan-Based Hydrogels Rich in Bilberry Fruit Extract |
| title_short | Anti-Aging Properties of Chitosan-Based Hydrogels Rich in Bilberry Fruit Extract |
| title_sort | anti aging properties of chitosan based hydrogels rich in bilberry fruit extract |
| topic | bilberry fruits blueberry fruit anti-aging potential topical preparation cosmetics chitosan |
| url | https://www.mdpi.com/2076-3921/13/1/105 |
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