Germinal proto-mitochondria from rat liver
A number of properties of the smallest (less than 0.2 μm) germinal proto-mitochondria (PRMC) from rat liver have been studied. These PRMC were obtained by filtering the light fraction of hepatic mitochondria (MC) through calibrated millipore membranes. Germinal PRMC contain in general the same prote...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2019-12-01
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| Series: | Biochemistry and Biophysics Reports |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405580819303243 |
| _version_ | 1819170851290873856 |
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| author | Nikolai Vekshin Vladimir Kovalev Alina Chaplygina |
| author_facet | Nikolai Vekshin Vladimir Kovalev Alina Chaplygina |
| author_sort | Nikolai Vekshin |
| collection | DOAJ |
| description | A number of properties of the smallest (less than 0.2 μm) germinal proto-mitochondria (PRMC) from rat liver have been studied. These PRMC were obtained by filtering the light fraction of hepatic mitochondria (MC) through calibrated millipore membranes. Germinal PRMC contain in general the same proteins as MC. However, they have the reduced content of flavoproteins and zero cytochrome oxidase. Germinal PRMC, in contrast to MC, almost does not contain the “aging pigment” - lipofuscin. They have DNA; the DNA/protein ratio in them is much higher than in MC, i.e. they are poor in protein. The obtained results support the earlier assumption that MC in specialized animal cells can arise from germinal PRMC - particles smaller than 0.2 μm containing DNA. It is assumed that the DNA molecules enter to cytoplasm during degradation of old MC serves as a seed for the formation of PRMC (with the connection of nuclear DNA). Keywords: Proto-mitochondria, Mitochondria, Post-mitochondria, Mitochondrial DNA, Flavoproteins, Respiratory chain, Lipofuscin |
| first_indexed | 2024-12-22T19:41:58Z |
| format | Article |
| id | doaj.art-6d4efbb0cd8f45a6b69f2753caeab3dd |
| institution | Directory Open Access Journal |
| issn | 2405-5808 |
| language | English |
| last_indexed | 2024-12-22T19:41:58Z |
| publishDate | 2019-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biochemistry and Biophysics Reports |
| spelling | doaj.art-6d4efbb0cd8f45a6b69f2753caeab3dd2022-12-21T18:14:50ZengElsevierBiochemistry and Biophysics Reports2405-58082019-12-0120Germinal proto-mitochondria from rat liverNikolai Vekshin0Vladimir Kovalev1Alina Chaplygina2Corresponding author.; Institute of Cell Biophysics of RAS, 142290, Pushchino, Moscow Region, Russian FederationInstitute of Cell Biophysics of RAS, 142290, Pushchino, Moscow Region, Russian FederationInstitute of Cell Biophysics of RAS, 142290, Pushchino, Moscow Region, Russian FederationA number of properties of the smallest (less than 0.2 μm) germinal proto-mitochondria (PRMC) from rat liver have been studied. These PRMC were obtained by filtering the light fraction of hepatic mitochondria (MC) through calibrated millipore membranes. Germinal PRMC contain in general the same proteins as MC. However, they have the reduced content of flavoproteins and zero cytochrome oxidase. Germinal PRMC, in contrast to MC, almost does not contain the “aging pigment” - lipofuscin. They have DNA; the DNA/protein ratio in them is much higher than in MC, i.e. they are poor in protein. The obtained results support the earlier assumption that MC in specialized animal cells can arise from germinal PRMC - particles smaller than 0.2 μm containing DNA. It is assumed that the DNA molecules enter to cytoplasm during degradation of old MC serves as a seed for the formation of PRMC (with the connection of nuclear DNA). Keywords: Proto-mitochondria, Mitochondria, Post-mitochondria, Mitochondrial DNA, Flavoproteins, Respiratory chain, Lipofuscinhttp://www.sciencedirect.com/science/article/pii/S2405580819303243 |
| spellingShingle | Nikolai Vekshin Vladimir Kovalev Alina Chaplygina Germinal proto-mitochondria from rat liver Biochemistry and Biophysics Reports |
| title | Germinal proto-mitochondria from rat liver |
| title_full | Germinal proto-mitochondria from rat liver |
| title_fullStr | Germinal proto-mitochondria from rat liver |
| title_full_unstemmed | Germinal proto-mitochondria from rat liver |
| title_short | Germinal proto-mitochondria from rat liver |
| title_sort | germinal proto mitochondria from rat liver |
| url | http://www.sciencedirect.com/science/article/pii/S2405580819303243 |
| work_keys_str_mv | AT nikolaivekshin germinalprotomitochondriafromratliver AT vladimirkovalev germinalprotomitochondriafromratliver AT alinachaplygina germinalprotomitochondriafromratliver |