Transcriptome and metabolomic analysis to reveal the browning spot formation of ‘Huangguan’ pear
Abstract Background Browning spot (BS) disorders seriously affect the appearance quality of ‘Huangguan’ pear and cause economic losses. Many studies on BS have mainly focused on physiological and biochemical aspects, and the molecular mechanism remains unclear. Results In the present study, the stru...
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| Format: | Article |
| Language: | English |
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BMC
2021-07-01
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| Series: | BMC Plant Biology |
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| Online Access: | https://doi.org/10.1186/s12870-021-03049-8 |
| _version_ | 1818655317788983296 |
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| author | Qi Wang Xinyi Wu Li Liu Daozhi Yao Jinchao Li Jie Fang Xiaonan Chen Liwu Zhu Pu Liu Zhenfeng Ye Bing Jia Wei Heng |
| author_facet | Qi Wang Xinyi Wu Li Liu Daozhi Yao Jinchao Li Jie Fang Xiaonan Chen Liwu Zhu Pu Liu Zhenfeng Ye Bing Jia Wei Heng |
| author_sort | Qi Wang |
| collection | DOAJ |
| description | Abstract Background Browning spot (BS) disorders seriously affect the appearance quality of ‘Huangguan’ pear and cause economic losses. Many studies on BS have mainly focused on physiological and biochemical aspects, and the molecular mechanism remains unclear. Results In the present study, the structural characteristics of ‘Huangguan’ pear with BS were observed via scanning electron microscopy (SEM), the water loss and brown spots were evaluated, and transcriptomic and metabolomics analyses were conducted to reveal the molecular mechanism underlying ‘Huangguan’ pear skin browning disorder. The results showed that the occurrence of BS was accompanied by a decrease in the wax layer and an increase in lignified cells. Genes related to wax biosynthesis were downregulated in BS, resulting in a decrease in the wax layer in BS. Genes related to lignin were upregulated at the transcriptional level, resulting in upregulation of metabolites related to phenylpropanoid biosynthesis. Expression of calcium-related genes were upregulated in BS. Cold-induced genes may represent the key genes that induce the formation of BS. In addition, the results demonstrated that exogenous NaH2PO4·2H2O and ABA treatment could inhibit the incidence of BS during harvest and storage time by increasing wax-related genes and calcium-related genes expression and increasing plant resistance, whereas the transcriptomics results indicated that GA3 may accelerate the incidence and index of BS. Conclusions The results of this study indicate a molecular mechanism that could explain BS formation and elucidate the effects of different treatments on the incidence and molecular regulation of BS. |
| first_indexed | 2024-12-17T03:07:46Z |
| format | Article |
| id | doaj.art-6d51b51cc2304da696bf3bcacbd5be29 |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-12-17T03:07:46Z |
| publishDate | 2021-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-6d51b51cc2304da696bf3bcacbd5be292022-12-21T22:05:54ZengBMCBMC Plant Biology1471-22292021-07-0121111810.1186/s12870-021-03049-8Transcriptome and metabolomic analysis to reveal the browning spot formation of ‘Huangguan’ pearQi Wang0Xinyi Wu1Li Liu2Daozhi Yao3Jinchao Li4Jie Fang5Xiaonan Chen6Liwu Zhu7Pu Liu8Zhenfeng Ye9Bing Jia10Wei Heng11College of Horticulture, Anhui Agricultural UniversityCollege of Horticulture, Anhui Agricultural UniversityCollege of Horticulture, Anhui Agricultural UniversityCollege of Horticulture, Anhui Agricultural UniversityCollege of Horticulture, Anhui Agricultural UniversityCollege of Horticulture, Anhui Agricultural UniversityCollege of Horticulture, Anhui Agricultural UniversityCollege of Horticulture, Anhui Agricultural UniversityCollege of Horticulture, Anhui Agricultural UniversityCollege of Horticulture, Anhui Agricultural UniversityCollege of Horticulture, Anhui Agricultural UniversityCollege of Horticulture, Anhui Agricultural UniversityAbstract Background Browning spot (BS) disorders seriously affect the appearance quality of ‘Huangguan’ pear and cause economic losses. Many studies on BS have mainly focused on physiological and biochemical aspects, and the molecular mechanism remains unclear. Results In the present study, the structural characteristics of ‘Huangguan’ pear with BS were observed via scanning electron microscopy (SEM), the water loss and brown spots were evaluated, and transcriptomic and metabolomics analyses were conducted to reveal the molecular mechanism underlying ‘Huangguan’ pear skin browning disorder. The results showed that the occurrence of BS was accompanied by a decrease in the wax layer and an increase in lignified cells. Genes related to wax biosynthesis were downregulated in BS, resulting in a decrease in the wax layer in BS. Genes related to lignin were upregulated at the transcriptional level, resulting in upregulation of metabolites related to phenylpropanoid biosynthesis. Expression of calcium-related genes were upregulated in BS. Cold-induced genes may represent the key genes that induce the formation of BS. In addition, the results demonstrated that exogenous NaH2PO4·2H2O and ABA treatment could inhibit the incidence of BS during harvest and storage time by increasing wax-related genes and calcium-related genes expression and increasing plant resistance, whereas the transcriptomics results indicated that GA3 may accelerate the incidence and index of BS. Conclusions The results of this study indicate a molecular mechanism that could explain BS formation and elucidate the effects of different treatments on the incidence and molecular regulation of BS.https://doi.org/10.1186/s12870-021-03049-8‘Huangguan’ pearBrowning disorderTranscriptomeMetabolomicMolecular mechanism |
| spellingShingle | Qi Wang Xinyi Wu Li Liu Daozhi Yao Jinchao Li Jie Fang Xiaonan Chen Liwu Zhu Pu Liu Zhenfeng Ye Bing Jia Wei Heng Transcriptome and metabolomic analysis to reveal the browning spot formation of ‘Huangguan’ pear BMC Plant Biology ‘Huangguan’ pear Browning disorder Transcriptome Metabolomic Molecular mechanism |
| title | Transcriptome and metabolomic analysis to reveal the browning spot formation of ‘Huangguan’ pear |
| title_full | Transcriptome and metabolomic analysis to reveal the browning spot formation of ‘Huangguan’ pear |
| title_fullStr | Transcriptome and metabolomic analysis to reveal the browning spot formation of ‘Huangguan’ pear |
| title_full_unstemmed | Transcriptome and metabolomic analysis to reveal the browning spot formation of ‘Huangguan’ pear |
| title_short | Transcriptome and metabolomic analysis to reveal the browning spot formation of ‘Huangguan’ pear |
| title_sort | transcriptome and metabolomic analysis to reveal the browning spot formation of huangguan pear |
| topic | ‘Huangguan’ pear Browning disorder Transcriptome Metabolomic Molecular mechanism |
| url | https://doi.org/10.1186/s12870-021-03049-8 |
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